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[182723_at]

Description E04F6.6 /REP_DB=WormBase Gene ID /WP=CE27909 /TR=Q19059 /GB=AAA68359.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_high_8_8 > 50      6grp_highest_nonorm > 61      6grp_total > 33      4grp_total > 62      6grp_high1_sub > 03      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 433
1 2 3 4 range
A 438.2
(3)
P 		301.2
(0.8)
P 		294.2
(0.7)
P 		62.3
(-3)
M 		376
B 446.5
(3)
P 		269.9
(0.1)
P 		253.2
(-0.2)
P 		81.7
(-3)
P 		365
C 494.9
(3)
P 		314.5
(0.3)
P 		367.9
(1.1)
P 		94.6
(-3)
A 		400
avr 459.9
(3) 		295.2
(0.4) 		305.1
(0.6) 		79.5
(-3) 		380

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 601
1 2 3 4 5 6 range
A 489.4
(1.6)
P 		454.4
(1.1)
P 		587.4
(3)
P 		455.9
(1.1)
P 		244.5
(-1.9)
P 		166.7
(-3)
P 		421
B 556.6
(0.7)
P 		505.8
(0.2)
P 		768
(3)
P 		393.5
(-1.1)
P 		280.6
(-2.3)
P 		213.7
(-3)
P 		554
C 577.4
(3)
P 		572.2
(2.9)
P 		557.9
(2.7)
P 		367.9
(-0.5)
P 		214.5
(-3)
P 		261.4
(-2.2)
P 		363
avr 541.1
(1.6) 		510.8
(1.2) 		637.8
(3) 		405.8
(-0.3) 		246.5
(-2.5) 		213.9
(-3) 		424
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 159.6
(3)
P 		65.4
(-0.8)
P 		76.9
(-0.3)
P 		9.1
(-3)
M 		151
B 142.4
(3)
P 		66.7
(-0.6)
P 		58.7
(-1)
P 		17.4
(-3)
P 		125
C 163.8
(3)
P 		90.4
(0)
P 		128.2
(1.6)
P 		15.9
(-3)
A 		148
avr 155.3
(3) 		74.2
(-0.4) 		87.9
(0.1) 		14.1
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 90.2
(-0.5)
P 		161.6
(3)
P 		127.9
(1.3)
P 		115.6
(0.7)
P 		73.8
(-1.4)
P 		40.6
(-3)
P 		121
B 193.7
(3)
P 		168.4
(1.7)
P 		186.5
(2.6)
P 		104.2
(-1.7)
P 		114
(-1.2)
P 		79.7
(-3)
P 		114
C 214
(3)
P 		174.6
(1.4)
P 		142
(0)
P 		74
(-2.8)
P 		69.4
(-3)
P 		87.5
(-2.2)
P 		145
avr 166
(2.9) 		168.2
(3) 		152.1
(2) 		97.9
(-1.3) 		85.7
(-2) 		69.3
(-3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182723_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5799
Sequence Type Exemplar sequence
Representative Public ID E04F6.6 NCBI
Target Description E04F6.6 /REP_DB=WormBase Gene ID /WP=CE27909 /TR=Q19059 /GB=AAA68359.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063094(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063094 NCBI Caenorhabditis elegans E04F6.6 (E04F6.6) mRNA, complete cds. 11/11 A 
SNAP00000032892 ENSEMBL cdna:SNAP chromosome:CEL140:II:7197452:7199296:-1 11/11 None
GENEFINDER00000032913 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7197452:7199296:-1 11/11 None
E04F6.6 ENSEMBL cdna:known chromosome:CEL140:II:7197346:7199496:-1 gene:E04F6.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7197447-7199228(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04F6.6
Entrez Gene 174182 Entrez gene
SwissProt Q19059 EMBL-EBI
WormBase CE27909 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68359 Hypothetical protein E04F6.6 [Caenorhabditis elegans] ref|NP_495495.1| E04F6.6 [Caenorhabditis elegans] 0.0
blast CAE67563 Hypothetical protein CBG13090 [Caenorhabditis briggsae] 0.0
blast CAE56005 Hypothetical protein CBG23555 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:182723_AT
agggatacttccatgattgtcaacgattccttccaaagtcagatccactttataatattg
catatgcatttgattcaaatgtaggtctcaacttgggaacatggagtgtgaaaggaattc
cgtactatccaattaacgaagaaggagcaattggagcaggaaggctcatgaatgttccat
ttggttcatggggaggaggttattccgatcatattggtgtaagagactattggtcacaat
atcaagaaatcggagcaaattggtatgaaggaaaatatggatataagtctggatggagtg
ttccacttgtacaatcacttggtattgaaggagacacacatgctgttgtgagtgttcccc
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGATACTTCCATGATTGTCAACG 620 57 767 Antisense
GATTGTCAACGATTCCTTCCAAAGT 424 435 781 Antisense
GTCAGATCCACTTTATAATATTGCA 202 467 804 Antisense
CAAATGTAGGTCTCAACTTGGGAAC 369 191 842 Antisense
GGAATTCCGTACTATCCAATTAACG 429 533 880 Antisense
GAGCAGGAAGGCTCATGAATGTTCC 138 389 920 Antisense
GAATGTTCCATTTGGTTCATGGGGA 615 329 936 Antisense
GGGAGGAGGTTATTCCGATCATATT 691 493 957 Antisense
AAGTCTGGATGGAGTGTTCCACTTG 554 157 1051 Antisense
GTTCCACTTGTACAATCACTTGGTA 19 441 1066 Antisense
CACATGCTGTTGTGAGTGTTCCCCT 640 195 1103 Antisense
Sequence Source Affymetrix Proprietary Database