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[182716_at]

Description W08F4.1 /REP_DB=WormBase Gene ID /WP=CE14712 /TR=O02114 /GB=AAC71168.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 34      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 2.5
(-3)
A 		12.3
(3)
A 		3.4
(-2.4)
A 		6.3
(-0.7)
A 		10
B 2.8
(-2)
A 		4.9
(1.6)
A 		5.7
(3)
A 		2.2
(-3)
A 		4
C 1.4
(-3)
A 		7.9
(-0.1)
A 		4.6
(-1.6)
A 		15
(3)
A 		14
avr 2.2
(-3) 		8.4
(3) 		4.6
(-0.7) 		7.8
(2.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 9.1
(3)
A 		6.4
(0.3)
A 		3.1
(-3)
A 		7.2
(1.1)
A 		5.4
(-0.7)
A 		3.5
(-2.6)
A 		6
B 5
(2.1)
A 		4.8
(1.7)
A 		2.8
(-2.1)
A 		3.2
(-1.3)
A 		5.5
(3)
A 		2.3
(-3)
A 		3
C 2.9
(1.4)
A 		3.2
(3)
A 		2.7
(0.3)
A 		2.1
(-3)
A 		3.2
(3)
A 		2.3
(-1.9)
A 		1
avr 5.7
(3) 		4.8
(1.2) 		2.9
(-2.6) 		4.2
(0) 		4.7
(1) 		2.7
(-3) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.9
(-3)
A 		2.7
(3)
A 		0.9
(-3)
A 		0.9
(-3)
A 		2
B 0.9
(0)
A 		1.2
(2.3)
A 		1.3
(3)
A 		0.5
(-3)
A 		1
C 0.5
(-3)
A 		2.3
(2.4)
A 		1.6
(0.3)
A 		2.5
(3)
A 		2
avr 0.8
(-3) 		2.1
(3) 		1.3
(-0.7) 		1.3
(-0.7) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 1.7
(0.8)
A 		2.3
(3)
A 		0.7
(-3)
A 		1.8
(1.1)
A 		1.6
(0.4)
A 		0.9
(-2.3)
A 		2
B 1.7
(1)
A 		1.6
(0.6)
A 		0.7
(-3)
A 		0.9
(-2.2)
A 		2.2
(3)
A 		0.8
(-2.6)
A 		2
C 1.1
(3)
A 		1
(2.1)
A 		0.7
(-0.4)
A 		0.4
(-3)
A 		1
(2.1)
A 		0.8
(0.4)
A 		1
avr 1.5
(2.3) 		1.6
(3) 		0.7
(-3) 		1
(-1) 		1.6
(3) 		0.8
(-2.3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182716_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5050
Sequence Type Exemplar sequence
Representative Public ID W08F4.1 NCBI
Target Description W08F4.1 /REP_DB=WormBase Gene ID /WP=CE14712 /TR=O02114 /GB=AAC71168.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061366(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061366 NCBI Caenorhabditis elegans W08F4.1 (W08F4.1) mRNA, complete cds. 11/11 None
SNAP00000029471 ENSEMBL cdna:SNAP chromosome:CEL140:II:593519:593858:1 11/11 None
SNAP00000036472 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2778774:2780663:1 9/11 None
GENEFINDER00000036602 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2774710:2780663:1 9/11 None
W08F4.1 ENSEMBL cdna:known chromosome:CEL140:II:593519:593858:1 gene:W08F4.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:593518-593858(+) 100.0 100.0
chrIV:2780336-2780669(+) 96.6 96.6
Public Domain and Genome References
Gene Title
Ensembl W08F4.1
Entrez Gene 189297 Entrez gene
SwissProt O02114 EMBL-EBI
WormBase CE14712 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY86298 Hypothetical protein Y54G2A.42 [Caenorhabditis elegans] 0.0
blast CAE56580 Hypothetical protein CBG24321 [Caenorhabditis briggsae] 2.0E-75
blast CAE56580 Hypothetical protein CBG24321 [Caenorhabditis briggsae] 1.0E-72
blast CAE57064 Hypothetical protein CBG24957 [Caenorhabditis briggsae] 4.0E-61
blast CAE57064 Hypothetical protein CBG24957 [Caenorhabditis briggsae] 3.0E-58
blast AAC71168 Hypothetical protein W08F4.1 [Caenorhabditis elegans] ref|NP_493767.1| W08F4.1 [Caenorhabditis elegans] 6.0E-43
blast AAY86298 Hypothetical protein Y54G2A.42 [Caenorhabditis elegans] 3.0E-40
Protein Domains
Database ID Description E-Value
scop a.4.5.MarR-like transcriptional regulators All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; MarR-like transcriptional regulators 8.0
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000036602 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:182716_AT
cgaaaatcgtcagacttccaccgtaccattgttgtctcaatcccatcgagctcatttgga
accagctgaaaagcttcctgcgcaagcatggaaaacttgacagcaaactggaaacagtcc
gtactcaagcgattcaatttcttaaaagcatcgacgaggaagctgtggcaaatacaatgg
atcacgttatggatgctgagattgaccttaagactattatggatgccgattttgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAAATCGTCAGACTTCCACCGTA 397 253 17 Antisense
TCCACCGTACCATTGTTGTCTCAAT 604 589 33 Antisense
TCTCAATCCCATCGAGCTCATTTGG 485 613 51 Antisense
CGAGCTCATTTGGAACCAGCTGAAA 87 251 63 Antisense
GAAAAGCTTCCTGCGCAAGCATGGA 204 355 84 Antisense
AACTGGAAACAGTCCGTACTCAAGC 348 137 122 Antisense
TCCGTACTCAAGCGATTCAATTTCT 314 595 134 Antisense
AAAGCATCGACGAGGAAGCTGTGGC 531 123 161 Antisense
GGCAAATACAATGGATCACGTTATG 544 533 183 Antisense
GGATGCTGAGATTGACCTTAAGACT 670 513 207 Antisense
GACTATTATGGATGCCGATTTTGAG 102 373 228 Antisense
Sequence Source Affymetrix Proprietary Database