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[182713_s_at]

Description C49G7.4 /REP_DB=WormBase Gene ID /WP=CE08862 /TR=O16225 /GB=AAK18899.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 05      6grp_total > 22      4grp_total > 52      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9103
1 2 3 4 range
A 8414.8
(2.3)
P 		9526.5
(3)
P 		7564.3
(1.7)
P 		453
(-3)
P 		9074
B 8599.1
(2.5)
P 		9346.2
(3)
P 		7980.1
(2.1)
P 		447.4
(-3)
P 		8899
C 8251.6
(2.3)
P 		9324.2
(3)
P 		7150
(1.5)
P 		423.1
(-3)
P 		8901
avr 8421.8
(2.3) 		9399
(3) 		7564.8
(1.8) 		441.2
(-3) 		8958

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7023
1 2 3 4 5 6 range
A 8755.7
(3)
P 		7235.6
(1.6)
P 		8371.9
(2.6)
P 		6359
(0.7)
P 		4564.5
(-1)
P 		2457.9
(-3)
P 		6298
B 9480.4
(3)
P 		8434.5
(2.1)
P 		9265.8
(2.8)
P 		6153.3
(0)
P 		5393.6
(-0.7)
P 		2819.3
(-3)
P 		6661
C 7717.3
(2.3)
P 		8346.5
(3)
P 		7559.8
(2.1)
P 		6866.9
(1.3)
P 		5630.8
(-0.1)
P 		3122.1
(-3)
P 		5224
avr 8651.1
(3) 		8005.5
(2.3) 		8399.2
(2.7) 		6459.7
(0.8) 		5196.3
(-0.5) 		2799.8
(-3) 		5851
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2998
1 2 3 4 range
A 3063.8
(3)
P 		2068
(1)
P 		1977.4
(0.8)
P 		66.3
(-3)
P 		2998
B 2742.2
(3)
P 		2308.3
(2)
P 		1849.2
(1)
P 		95.3
(-3)
P 		2647
C 2731.5
(3)
P 		2680.8
(2.9)
P 		2492
(2.5)
P 		71.3
(-3)
P 		2660
avr 2845.8
(3) 		2352.4
(1.9) 		2106.2
(1.4) 		77.6
(-3) 		2768

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2700
1 2 3 4 5 6 range
A 1614
(0.1)
P 		2573.3
(3)
P 		1823.2
(0.7)
P 		1612.8
(0.1)
P 		1377.4
(-0.6)
P 		598.4
(-3)
P 		1975
B 3298.7
(3)
P 		2807.5
(1.7)
P 		2250
(0.2)
P 		1629.8
(-1.5)
P 		2191.3
(0)
P 		1051.3
(-3)
P 		2247
C 2860.5
(3)
P 		2546.8
(2)
P 		1924.1
(-0.1)
P 		1380.9
(-1.9)
P 		1822
(-0.4)
P 		1045.1
(-3)
P 		1815
avr 2591.1
(2.8) 		2642.5
(3) 		1999.1
(0.8) 		1541.2
(-0.8) 		1796.9
(0.1) 		898.3
(-3) 		1744
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182713_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14922
Sequence Type Exemplar sequence
Representative Public ID C49G7.4 NCBI
Target Description C49G7.4 /REP_DB=WormBase Gene ID /WP=CE08862 /TR=O16225 /GB=AAK18899.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071729(11), NM_071728(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071729 NCBI Caenorhabditis elegans C49G7.3 (C49G7.3) mRNA, complete cds. 11/11 A 
NM_071728 NCBI Caenorhabditis elegans C49G7.4 (C49G7.4) mRNA, complete cds. 11/11 A 
SNAP00000008207 ENSEMBL cdna:SNAP chromosome:CEL140:V:4043766:4044610:1 11/11 None
SNAP00000008208 ENSEMBL cdna:SNAP chromosome:CEL140:V:4046255:4047099:1 11/11 None
GENEFINDER00000008213 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4043766:4044610:1 11/11 None
GENEFINDER00000008220 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4046255:4047099:1 11/11 None
C49G7.4 ENSEMBL cdna:known chromosome:CEL140:V:4043729:4044764:1 gene:C49G7.4 11/11 A 
C49G7.3 ENSEMBL cdna:known chromosome:CEL140:V:4046218:4047249:1 gene:C49G7.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4043765-4044610(+) 100.0 100.0
chrV:4046254-4047099(+) 93.57 93.57
Public Domain and Genome References
Gene Title

Ensembl C49G7.4
C49G7.3
Entrez Gene 178809 Entrez gene
178810 Entrez gene
SwissProt O16226 EMBL-EBI
O16225 EMBL-EBI
WormBase CE08861 Wormbase
CE08862 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18899 Hypothetical protein C49G7.4 [Caenorhabditis elegans] ref|NP_504129.1| C49G7.4 [Caenorhabditis elegans] 1.0E-130
blast AAK18898 Hypothetical protein C49G7.3 [Caenorhabditis elegans] ref|NP_504130.1| C49G7.3 [Caenorhabditis elegans] 1.0E-129
blast AAK18899 Hypothetical protein C49G7.4 [Caenorhabditis elegans] ref|NP_504129.1| C49G7.4 [Caenorhabditis elegans] 1.0E-126
blast AAK18898 Hypothetical protein C49G7.3 [Caenorhabditis elegans] ref|NP_504130.1| C49G7.3 [Caenorhabditis elegans] 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.5
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.3E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.9E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.3E-9
Sequence
Target Sequence 
>C. ELEGANS:182713_S_AT
cggttacccagctccaggaaatgatgttgaaagaccatttgcttgctacacaactgccac
tccaactcctgctccagttgttccagacatgagatctgctgctttgaaaaattgtgccag
gacttgtggattgtgctgtaagacggctgactttgactgcccaaatgttcaattcccacg
tctgacgtgctccaccattaccaatgaacagtgccgttcttctgtctggcgtaccatcat
cgctactgattgcccatcagcctgcggattctgtaaccaaggaggatgtgtcgatggagt
catggattgtgctaatgatctttccatttgtaactcagttggaatgcaagaatttgtcaa
ccaatactgtcaaagaacttgtaacagatgttcttccagcactgcagccacaggcactgg
aggatcatgcggaggaagatatattgctgattccagcacagcttgtgcagcatggaagtt
gaacggattctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGTTACCCAGCTCCAGGAAATGAT 283 243 201 Antisense
CTGCTCCAGTTGTTCCAGACATGAG 550 237 269 Antisense
GTTCCAGACATGAGATCTGCTGCTT 445 441 280 Antisense
GACATGAGATCTGCTGCTTTGAAAA 333 369 286 Antisense
CCAAATGTTCAATTCCCACGTCTGA 144 275 361 Antisense
GAGGATGTGTCGATGGAGTCATGGA 632 403 482 Antisense
GCCACAGGCACTGGAGGATCATGCG 701 277 607 Antisense
TGCGGAGGAAGATATATTGCTGATT 280 581 628 Antisense
TGCTGATTCCAGCACAGCTTGTGCA 240 581 645 Antisense
GCACAGCTTGTGCAGCATGGAAGTT 430 317 656 Antisense
GCATGGAAGTTGAACGGATTCTGCA 173 311 670 Antisense
Sequence Source Affymetrix Proprietary Database