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[182709_at]

Description F28F9.2 /REP_DB=WormBase Gene ID /WP=CE09763 /TR=Q94195 /GB=AAB09123.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 12      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 51.2
(-2.3)
A 		54.8
(-2.2)
A 		25.4
(-3)
A 		239.4
(3)
A 		214
B 66.4
(-1.2)
A 		58.8
(-1.8)
A 		44.9
(-3)
A 		116
(3)
A 		71
C 111.6
(3)
A 		75.8
(-0.3)
A 		46.5
(-3)
A 		65.9
(-1.2)
A 		65
avr 76.4
(-0.8) 		63.1
(-1.6) 		38.9
(-3) 		140.4
(3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 78.1
(2.8)
A 		14.1
(-3)
A 		53
(0.5)
A 		80.1
(3)
A 		15.1
(-2.9)
A 		63.6
(1.5)
A 		66
B 48.2
(-2)
A 		41.3
(-3)
A 		82.9
(3)
A 		46
(-2.3)
A 		75.9
(2)
A 		51.2
(-1.6)
A 		42
C 11.6
(-3)
A 		48.5
(-0.4)
A 		12.4
(-2.9)
A 		47.1
(-0.5)
A 		97.2
(3)
A 		77.3
(1.6)
A 		86
avr 46
(-0.7) 		34.6
(-3) 		49.4
(0) 		57.7
(1.7) 		62.7
(2.7) 		64
(3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 18.6
(-0.5)
A 		11.9
(-1.9)
A 		6.6
(-3)
A 		35
(3)
A 		28
B 21.2
(1.5)
A 		14.5
(-1.3)
A 		10.4
(-3)
A 		24.7
(3)
A 		14
C 37
(3)
A 		21.8
(-0.5)
A 		16.2
(-1.8)
A 		11.1
(-3)
A 		26
avr 25.6
(3) 		16.1
(-0.9) 		11.1
(-3) 		23.6
(2.2) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 14.4
(0.7)
A 		5
(-2.8)
A 		11.5
(-0.4)
A 		20.3
(3)
A 		4.6
(-3)
A 		15.5
(1.2)
A 		16
B 16.8
(-1.5)
A 		13.7
(-2.5)
A 		20.1
(-0.5)
A 		12.2
(-3)
A 		30.8
(3)
A 		19.1
(-0.8)
A 		19
C 4.3
(-2.8)
A 		14.8
(-0.5)
A 		3.2
(-3)
A 		9.5
(-1.7)
A 		31.5
(3)
A 		25.9
(1.8)
A 		28
avr 11.8
(-2.7) 		11.2
(-3) 		11.6
(-2.8) 		14
(-1.5) 		22.3
(3) 		20.2
(1.9) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182709_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10454
Sequence Type Exemplar sequence
Representative Public ID F28F9.2 NCBI
Target Description F28F9.2 /REP_DB=WormBase Gene ID /WP=CE09763 /TR=Q94195 /GB=AAB09123.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068022(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068022 NCBI Caenorhabditis elegans F28F9.2 (F28F9.2) mRNA, complete cds. 11/11 None
F28F9.2 ENSEMBL cdna:known chromosome:CEL140:IV:3838894:3841622:1 gene:F28F9.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002093 5/11 Cross Hyb Matching Probes None
GENEFINDER00000002097 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3838899-3841628(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28F9.2
Entrez Gene 185077 Entrez gene
SwissProt Q94195 EMBL-EBI
WormBase CE09763 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB09123 Hypothetical protein F28F9.2 [Caenorhabditis elegans] ref|NP_500423.1| F28F9.2 [Caenorhabditis elegans] 1.0E-125
blast CAE65669 Hypothetical protein CBG10735 [Caenorhabditis briggsae] 5.0E-35
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500423.1 1 152-174
F28F9.2 1 152-174
Sequence
Target Sequence 
>C. ELEGANS:182709_AT
actttggagaggcttcatcggaccctcgcttggattcgagaagcgcaagattctatgtta
catgaaaatctgcgtaatttttttcagaacttcataaaatgtctttcccccaaattgttt
tctcctggcaacacgacaaatctcctaggagccggcggagcagagctcccactgactcaa
ctttatccccaaattttgtattttcatctcaatttccaaactaaaaaatgtctattgaca
atgatgatgcgacggtgtgcattatcaacagcacaatgcccatgtaccagccaaacattt
ttctgtgctcgccgggctcttcgtgctgcactcgtcggggaattatcgcttgctgtcaga
atgatgtgtcgtttagtcaggttgaagcagtctctaccgtttctcgtcttgtggctcatc
atttttggaattgccacattggtacacttgttctttgaagacgatgaaactggaaagtcg
attaataaggagaagccgttgaacggaaaaatggaactgcttctcccaatcgaaaacgac
tacacagtggaggatccagtgtttggcaagctctac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTGGAGAGGCTTCATCGGACCC 490 99 79 Antisense
TCGGACCCTCGCTTGGATTCGAGAA 33 607 96 Antisense
CATAAAATGTCTTTCCCCCAAATTG 318 195 171 Antisense
TTTTCTCCTGGCAACACGACAAATC 300 677 196 Antisense
AAATCTCCTAGGAGCCGGCGGAGCA 474 115 216 Antisense
TGACTCAACTTTATCCCCAAATTTT 440 569 251 Antisense
GCTGCACTCGTCGGGGAATTATCGC 353 297 403 Antisense
TCGTCTTGTGGCTCATCATTTTTGG 355 603 482 Antisense
GCCACATTGGTACACTTGTTCTTTG 187 279 511 Antisense
AAAATGGAACTGCTTCTCCCAATCG 661 131 586 Antisense
GGATCCAGTGTTTGGCAAGCTCTAC 104 513 630 Antisense
Sequence Source Affymetrix Proprietary Database