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[182703_s_at]

Description T27A3.1 /REP_DB=WormBase Gene ID /WP=CE14217 /TR=P91503 /GB=AAB42296.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 21      6grp_total > 24      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1816
1 2 3 4 range
A 2336.9
(3)
P 		1462.9
(-0.4)
P 		1288.8
(-1)
P 		775.3
(-3)
P 		1562
B 2591.1
(3)
P 		1234.1
(-1.5)
P 		1260.2
(-1.4)
P 		777.4
(-3)
P 		1814
C 2203
(3)
P 		1213.4
(-1.3)
P 		1408.6
(-0.4)
P 		817.8
(-3)
P 		1385
avr 2377
(3) 		1303.5
(-1.1) 		1319.2
(-1) 		790.2
(-3) 		1587

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1047
1 2 3 4 5 6 range
A 1437.4
(2.1)
P 		1573.8
(3)
P 		1200.9
(0.4)
P 		986.6
(-1)
P 		1029.2
(-0.7)
P 		702.1
(-3)
P 		872
B 1632.1
(3)
P 		1461
(1.3)
P 		1479.3
(1.5)
P 		1339
(0)
P 		1210.8
(-1.3)
P 		1037.6
(-3)
P 		595
C 1748.8
(3)
P 		1642.8
(2.1)
P 		1357.4
(-0.2)
P 		1155.9
(-1.8)
P 		1103.2
(-2.3)
P 		1011.6
(-3)
P 		737
avr 1606.1
(3) 		1559.2
(2.6) 		1345.9
(0.7) 		1160.5
(-0.9) 		1114.4
(-1.3) 		917.1
(-3) 		689
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 737
1 2 3 4 range
A 850.9
(3)
P 		317.6
(-1.3)
P 		336.9
(-1.2)
P 		113.5
(-3)
P 		737
B 826.3
(3)
P 		304.8
(-1.7)
P 		292
(-1.9)
P 		165.7
(-3)
P 		661
C 729.2
(3)
P 		348.9
(-0.9)
P 		490.9
(0.6)
P 		137.9
(-3)
P 		591
avr 802.1
(3) 		323.8
(-1.3) 		373.3
(-0.9) 		139
(-3) 		663

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 477
1 2 3 4 5 6 range
A 265
(-1.5)
P 		559.7
(3)
P 		261.5
(-1.6)
P 		250.2
(-1.8)
P 		310.6
(-0.8)
P 		170.9
(-3)
P 		389
B 567.9
(3)
P 		486.3
(0.7)
P 		359.2
(-2.9)
P 		354.6
(-3)
P 		491.9
(0.9)
P 		386.9
(-2.1)
P 		213
C 648.2
(3)
P 		501.3
(0.9)
P 		345.5
(-1.4)
P 		232.5
(-3)
P 		357
(-1.2)
P 		338.7
(-1.5)
P 		416
avr 493.7
(2.4) 		515.8
(3) 		322.1
(-1.9) 		279.1
(-3) 		386.5
(-0.3) 		298.8
(-2.5) 		237
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182703_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.932
Sequence Type Exemplar sequence
Representative Public ID T27A3.1 NCBI
Target Description T27A3.1 /REP_DB=WormBase Gene ID /WP=CE14217 /TR=P91503 /GB=AAB42296.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170872(11), NM_170873(9), NM_059365(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170872 NCBI Caenorhabditis elegans T27A3.1a (T27A3.1) mRNA, complete cds. 11/11 None
NM_170873 NCBI Caenorhabditis elegans T27A3.1b (T27A3.1) mRNA, complete cds. 9/11 None
NM_059365 NCBI Caenorhabditis elegans T27A3.1c (T27A3.1) mRNA, complete cds. 11/11 None
T27A3.1b ENSEMBL cdna:known chromosome:CEL140:I:6130725:6134943:1 gene:T27A3.1 9/11 None
T27A3.1a ENSEMBL cdna:known chromosome:CEL140:I:6130725:6134986:1 gene:T27A3.1 11/11 None
T27A3.1c ENSEMBL cdna:known chromosome:CEL140:I:6131285:6134667:1 gene:T27A3.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182088 8/11 Cross Hyb Matching Probes None
NM_182087 7/11 Cross Hyb Matching Probes None
SNAP00000022772 8/11 Cross Hyb Matching Probes None
T27A3.1e 8/11 Cross Hyb Matching Probes None
T27A3.1d.1 7/11 Cross Hyb Matching Probes None
T27A3.1d.2 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172297 5 3 NM_059365 182703_s_at (11)
NM_170872 182703_s_at (11)
NM_170873 182703_s_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6130724-6134667(+) 100.0 100.0
Public Domain and Genome References
Ensembl T27A3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB42296 Hypothetical protein T27A3.1a [Caenorhabditis elegans] ref|NP_740861.1| T27A3.1a [Caenorhabditis elegans] 0.0
blast AAO21401 Hypothetical protein T27A3.1c [Caenorhabditis elegans] ref|NP_491766.2| T27A3.1c [Caenorhabditis elegans] 0.0
blast AAO21403 Hypothetical protein T27A3.1e [Caenorhabditis elegans] ref|NP_871888.1| T27A3.1e [Caenorhabditis elegans] 0.0
blast AAM22071 Hypothetical protein T27A3.1b [Caenorhabditis elegans] ref|NP_740862.1| T27A3.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006933 EMBL-EBI HAP1, N-terminal 1.0E-126
Pfam IPR006933 EMBL-EBI HAP1, N-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:182703_S_AT
ccgtgtccacaccgattgtggcacgaaagtatatcccgtgtagcagcccgagtaagagca
atttgagcaagttacaacggttgcatataggtaatcgtccacatgctcaaagtttcgatg
ttccatctacaactatttatgacgcatcgtcttacgcagagtcaaaactaggacaaccgg
gaatgcctggaactcgtgatctggatatctccttgaaagtaatcaaggctagggaagagg
tgcaaaaagagtttgcggcgttttgtcagcggaaaggtatcgatcagcaacagtttttcg
gttctcaggaccgtcgtcaatcaaatgaaacatcatggatggatttcagtgctgcatacg
gagtcagaatgattcagagtctgaaaccaagacatgccgaacagtcattagcattgggtc
tcagacaaggagtgttgacgagagcagagcttctcacgtctccatcaacatcacctcaac
atcgaagtttatcactctcattatcacctggaacatctgacggtggtctcacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTGTCCACACCGATTGTGGCACG 476 265 1265 Antisense
TCCCGTGTAGCAGCCCGAGTAAGAG 609 595 1298 Antisense
ATAGGTAATCGTCCACATGCTCAAA 459 21 1351 Antisense
GACGCATCGTCTTACGCAGAGTCAA 538 379 1405 Antisense
GACAACCGGGAATGCCTGGAACTCG 45 359 1436 Antisense
GAACTCGTGATCTGGATATCTCCTT 342 349 1454 Antisense
TTTGCGGCGTTTTGTCAGCGGAAAG 206 667 1516 Antisense
CAGCAACAGTTTTTCGGTTCTCAGG 160 203 1549 Antisense
GTTCTCAGGACCGTCGTCAATCAAA 97 443 1565 Antisense
TATCACTCTCATTATCACCTGGAAC 622 659 1754 Antisense
GGAACATCTGACGGTGGTCTCACGA 424 529 1774 Antisense
Sequence Source Affymetrix Proprietary Database