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[182702_at]

Description K08B4.5 /REP_DB=WormBase Gene ID /WP=CE18034 /TR=Q9TYY3 /GB=AAC68982.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 60      4grp_total > 28      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 8.3
(3)
A 		4.2
(-0.8)
A 		1.9
(-3)
A 		4
(-1)
A 		6
B 3
(-2.3)
A 		14.3
(3)
A 		1.5
(-3)
A 		3.9
(-1.9)
A 		13
C 3
(-2.4)
A 		2.7
(-2.6)
A 		2
(-3)
A 		11.9
(3)
A 		10
avr 4.8
(0.4) 		7.1
(3) 		1.8
(-3) 		6.6
(2.4) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 5.5
(1.5)
A 		6.4
(2.4)
A 		7
(3)
A 		5.5
(1.5)
A 		2.2
(-1.8)
A 		1
(-3)
A 		6
B 2
(-2)
A 		5.7
(3)
A 		1.3
(-3)
A 		5.1
(2.2)
A 		1.6
(-2.6)
A 		2.5
(-1.4)
A 		4
C 2.6
(-2.7)
A 		3.6
(-2.2)
A 		10.5
(1)
A 		14.9
(3)
A 		1.9
(-3)
A 		10.6
(1)
A 		13
avr 3.4
(-1.6) 		5.2
(0) 		6.3
(1) 		8.5
(3) 		1.9
(-3) 		4.7
(-0.5) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 3
(3)
A 		0.9
(-2)
A 		0.5
(-3)
A 		0.6
(-2.8)
A 		3
B 0.9
(-1.9)
A 		3.5
(3)
A 		0.3
(-3)
A 		0.8
(-2.1)
A 		3
C 1
(-1.6)
A 		0.8
(-2.5)
A 		0.7
(-3)
A 		2
(3)
A 		1
avr 1.6
(2.5) 		1.7
(3) 		0.5
(-3) 		1.1
(0) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 1
(-0.7)
A 		2.3
(3)
A 		1.5
(0.7)
A 		1.4
(0.4)
A 		0.7
(-1.6)
A 		0.2
(-3)
A 		2
B 0.7
(-1.5)
A 		1.9
(3)
A 		0.3
(-3)
A 		1.3
(0.8)
A 		0.6
(-1.9)
A 		0.9
(-0.8)
A 		2
C 1
(-2.2)
A 		1.1
(-2)
A 		2.7
(1.2)
A 		3
(1.8)
A 		0.6
(-3)
A 		3.6
(3)
A 		3
avr 0.9
(-1.6) 		1.8
(2.5) 		1.5
(1.2) 		1.9
(3) 		0.6
(-3) 		1.6
(1.6) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182702_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10433
Sequence Type Exemplar sequence
Representative Public ID K08B4.5 NCBI
Target Description K08B4.5 /REP_DB=WormBase Gene ID /WP=CE18034 /TR=Q9TYY3 /GB=AAC68982.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068513(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068513 NCBI Caenorhabditis elegans K08B4.5 (K08B4.5) mRNA, complete cds. 10/11 None
K08B4.5 ENSEMBL cdna:known chromosome:CEL140:IV:6086333:6089387:-1 gene:K08B4.5 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004905 6/11 Cross Hyb Matching Probes None
GENEFINDER00000004914 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6085844-6089393(-) 89.91 99.93
Public Domain and Genome References
Gene Title
Ensembl K08B4.5
Entrez Gene 187131 Entrez gene
SwissProt Q9TYY3 EMBL-EBI
WormBase CE36600 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68982 Hypothetical protein K08B4.5 [Caenorhabditis elegans] ref|NP_500914.2| K08B4.5 [Caenorhabditis elegans] 0.0
blast CAD29326 Hypothetical protein VY10G11R.1 [Caenorhabditis elegans] ref|NP_741498.1| VY10G11R.1 [Caenorhabditis elegans] 6.0E-13
blast CAA87786 Hypothetical protein K02C4.3 [Caenorhabditis elegans] ref|NP_495696.1| K02C4.3 [Caenorhabditis elegans] sp|Q09931|UBPY_CAEEL Probable ubiquitin carboxyl-terminal hydrolase K02C4.3 (Ubiquitin thiolesterase) (Ubiquitin-specific processing protease) (Deubiquitinating enzyme) 3.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.9E-4
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 7.4E-14
Sequence
Target Sequence 
>C. ELEGANS:182702_AT
cagaaagtgctgccagatgctccgaactttcctgaatgcctcgaagcatatttcatcaat
gatattccaacagaaatatgtcaaaagttgcaaagctctgcaaaagttcgtgtggacaac
tattgcagaattgtcgaatacgagtccaagacgttgacccctgtccgtcgtcgcccaaat
cctcctttcactcaatctggcaaaaaagaaaatggtttctggcacgaggatcttcataaa
ggaaacattttacttgcggtcaccgataaggattttatcaaatatcgagtcgataagaag
cttatcaaactgcgtactaatggactcatggaaacatcactgacttcatgttcttaccgg
ataatgaaggagctctcaaaaaagttattcgaggcgaaaacaatcgaagaattttggaat
tatcaggacttgttggaaagtgttattgtaattgataagcccaacaatatcaaccatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAAAGTGCTGCCAGATGCTCCGA 400 205 880 Antisense
CAGATGCTCCGAACTTTCCTGAATG 125 207 893 Antisense
GAACTTTCCTGAATGCCTCGAAGCA 613 349 903 Antisense
AAGCTCTGCAAAAGTTCGTGTGGAC 313 149 972 Antisense
ATACGAGTCCAAGACGTTGACCCCT 142 1 1017 Antisense
ATGGTTTCTGGCACGAGGATCTTCA 228 51 1091 Antisense
AAACATTTTACTTGCGGTCACCGAT 117 129 1122 Antisense
CAAACTGCGTACTAATGGACTCATG 678 191 1185 Antisense
GACTCATGGAAACATCACTGACTTC 618 373 1202 Antisense
CACTGACTTCATGTTCTTACCGGAT 168 197 1217 Antisense
ATAAGCCCAACAATATCAACCATAT 699 23 1334 Antisense
Sequence Source Affymetrix Proprietary Database