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[182685_at]

Description C36B7.7 /REP_DB=WormBase Gene ID /WP=CE04178 /GEN=hen-1 /GB=AAK68234.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 88      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 91      6grp_total > 78      4grp_total > 13      6grp_highest_sub > Da      6grp_cgc > 59      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 132.8
(-0.2)
P 		111.5
(-1.2)
P 		69
(-3)
P 		207.2
(3)
P 		138
B 134.2
(0.3)
P 		120.1
(-0.3)
P 		63.3
(-3)
P 		191.8
(3)
P 		129
C 183.5
(2.2)
P 		93.9
(-3)
P 		99.8
(-2.7)
P 		196.6
(3)
P 		103
avr 150.2
(0.6) 		108.5
(-1.5) 		77.4
(-3) 		198.5
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 102.9
(-2.4)
P 		82.9
(-2.9)
P 		80.7
(-3)
P 		79.7
(-3)
P 		191
(-0.4)
P 		332.4
(3)
P 		253
B 88.5
(-2.4)
P 		116.7
(-1.5)
P 		134
(-1)
P 		67.1
(-3)
P 		197.4
(0.8)
P 		272
(3)
P 		205
C 133.1
(-1.6)
P 		128.9
(-1.7)
P 		67.7
(-3)
P 		97.2
(-2.4)
P 		211.5
(0.1)
P 		344.4
(3)
P 		277
avr 108.2
(-2.3) 		109.5
(-2.3) 		94.1
(-2.7) 		81.3
(-3) 		200
(0) 		316.3
(3) 		235
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 48.4
(3)
P 		24.2
(-1.8)
P 		18
(-3)
P 		30.3
(-0.6)
P 		30
B 42.8
(3)
P 		29.7
(0.2)
P 		14.7
(-3)
P 		40.9
(2.6)
P 		28
C 60.7
(3)
P 		27
(-3)
P 		34.8
(-1.6)
P 		33.2
(-1.9)
P 		34
avr 50.6
(3) 		27
(-2) 		22.5
(-3) 		34.8
(-0.4) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 19
(-2.9)
P 		29.5
(-1.9)
P 		17.6
(-3)
P 		20.2
(-2.8)
P 		57.6
(0.8)
P 		80.9
(3)
P 		63
B 30.8
(-2.1)
P 		38.9
(-1.5)
P 		32.5
(-1.9)
P 		17.8
(-3)
P 		80.2
(1.5)
P 		101.4
(3)
P 		84
C 49.3
(-1)
P 		39.3
(-1.6)
P 		17.2
(-3)
P 		19.6
(-2.9)
P 		68.4
(0.1)
P 		115.3
(3)
P 		98
avr 33
(-2) 		35.9
(-1.7) 		22.4
(-2.8) 		19.2
(-3) 		68.7
(0.7) 		99.2
(3) 		80
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182685_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16828
Sequence Type Exemplar sequence
Representative Public ID C36B7.7 NCBI
Target Description C36B7.7 /REP_DB=WormBase Gene ID /WP=CE04178 /GEN=hen-1 /GB=AAK68234.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076799(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076799 NCBI Caenorhabditis elegans HEsitatioN behavior family member (hen-1) (hen-1) mRNA, complete cds. 11/11 None
SNAP00000024840 ENSEMBL cdna:SNAP chromosome:CEL140:X:7116369:7116931:-1 11/11 None
GENEFINDER00000024844 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7116369:7116931:-1 11/11 None
C36B7.7 ENSEMBL cdna:known chromosome:CEL140:X:7116153:7116944:-1 gene:C36B7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7116367-7116930(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hen-1
Ensembl C36B7.7
Entrez Gene 191666 Entrez gene
SwissProt Q966P4 EMBL-EBI
WormBase CE04178 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509200 HEsitatioN behavior family member (hen-1) [Caenorhabditis elegans] gb|AAK68234.1| Hesitation behavior protein 1 [Caenorhabditis elegans] dbj|BAC00782.1| HEN-1 [Caenorhabditis elegans] 4.0E-68
blast CAE65845 Hypothetical protein CBG10976 [Caenorhabditis briggsae] 2.0E-39
Sequence
Target Sequence 
>C. ELEGANS:182685_AT
ttcgtatgtgcactttgctcatccgttttgtcatctcaagatggagcatttggtcacgga
aatcatcggccactgaatgatgcagatgctttgactgcaataaaaccagtttgtgaaact
gaccgtcatggcaacaagtacataccatgtcccatccatgacataaatgaccctcgcaac
tggccatgtatcaaactggctgatttgtgtaatggaaaagctgattgtccaaacggagac
gacgaaaactacttccaatgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGTATGTGCACTTTGCTCATCCG 692 695 34 Antisense
CTCATCCGTTTTGTCATCTCAAGAT 462 229 51 Antisense
ATCTCAAGATGGAGCATTTGGTCAC 107 33 66 Antisense
ATTTGGTCACGGAAATCATCGGCCA 250 17 81 Antisense
ATCGGCCACTGAATGATGCAGATGC 284 35 98 Antisense
AGTTTGTGAAACTGACCGTCATGGC 391 61 141 Antisense
TACATACCATGTCCCATCCATGACA 233 645 172 Antisense
ATGACATAAATGACCCTCGCAACTG 588 43 191 Antisense
CCTCGCAACTGGCCATGTATCAAAC 455 267 205 Antisense
GCTGATTGTCCAAACGGAGACGACG 469 293 253 Antisense
GACGACGAAAACTACTTCCAATGTT 703 377 271 Antisense
Sequence Source Affymetrix Proprietary Database