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[182682_at]

Description R12A1.2 /REP_DB=WormBase Gene ID /WP=CE12780 /TR=O16700 /GB=AAB66163.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 61      6grp_total > 13      4grp_total > 39      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 654
1 2 3 4 range
A 822.7
(3)
P 		168.8
(-3)
P 		175.4
(-2.9)
P 		338.7
(-1.4)
P 		654
B 724.9
(3)
P 		270.4
(-2.4)
P 		219.3
(-3)
P 		305.7
(-2)
P 		506
C 773.8
(3)
P 		323.6
(-1.6)
P 		188.2
(-3)
P 		387.9
(-1)
P 		586
avr 773.8
(3) 		254.3
(-2.4) 		194.3
(-3) 		344.1
(-1.4) 		580

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 534
(2.3)
P 		482.7
(1.4)
P 		571.1
(3)
P 		383.2
(-0.5)
P 		360
(-0.9)
P 		245.5
(-3)
P 		326
B 491.6
(1.8)
P 		558.6
(3)
P 		397.1
(-0)
P 		475.3
(1.4)
P 		301.6
(-1.8)
P 		236.2
(-3)
P 		322
C 380.2
(-0.7)
P 		580.8
(3)
P 		387.9
(-0.6)
P 		516.8
(1.8)
P 		293
(-2.4)
P 		259
(-3)
P 		322
avr 468.6
(1.5) 		540.7
(3) 		452
(1.2) 		458.4
(1.3) 		318.2
(-1.5) 		246.9
(-3) 		294
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 299.5
(3)
P 		36.6
(-3)
P 		45.8
(-2.8)
P 		49.6
(-2.7)
P 		263
B 231.2
(3)
P 		66.8
(-2.5)
P 		50.8
(-3)
P 		65.1
(-2.5)
P 		180
C 256.1
(3)
P 		93
(-2.1)
P 		65.6
(-3)
P 		65.4
(-3)
P 		191
avr 262.3
(3) 		65.5
(-2.7) 		54.1
(-3) 		60
(-2.8) 		208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 98.4
(-0.9)
P 		171.7
(3)
P 		124.4
(0.5)
P 		97.2
(-1)
P 		108.6
(-0.4)
P 		59.8
(-3)
P 		112
B 171
(2.1)
P 		185.9
(3)
P 		96.4
(-2.5)
P 		125.9
(-0.7)
P 		122.5
(-0.9)
P 		88.1
(-3)
P 		98
C 140.9
(0.6)
P 		177.2
(3)
P 		98.7
(-2.2)
P 		103.9
(-1.9)
P 		94.8
(-2.5)
P 		86.7
(-3)
P 		91
avr 136.8
(0.5) 		178.3
(3) 		106.5
(-1.3) 		109
(-1.2) 		108.6
(-1.2) 		78.2
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182682_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14975
Sequence Type Exemplar sequence
Representative Public ID R12A1.2 NCBI
Target Description R12A1.2 /REP_DB=WormBase Gene ID /WP=CE12780 /TR=O16700 /GB=AAB66163.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071008(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071008 NCBI Caenorhabditis elegans SyNapTotagmin family member (snt-5) (snt-5) mRNA, complete cds. 11/11 None
GENEFINDER00000007198 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1393195:1402592:-1 11/11 None
R12A1.2.1 ENSEMBL cdna:known chromosome:CEL140:V:1392954:1411445:-1 gene:R12A1.2 11/11 None
R12A1.2.2 ENSEMBL cdna:known chromosome:CEL140:V:1393195:1411447:-1 gene:R12A1.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1393194-1395930(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol snt-5
Ensembl R12A1.2
Entrez Gene 178631 Entrez gene
SwissProt O16700 EMBL-EBI
WormBase CE29448 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8021 synaptic vesicle inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66163 Synaptotagmin protein 5 [Caenorhabditis elegans] ref|NP_503409.1| SyNapTotagmin family member (snt-5) [Caenorhabditis elegans] 0.0
blast CAE62345 Hypothetical protein CBG06421 [Caenorhabditis briggsae] 0.0
blast NP_945343 synaptotagmin 10 [Homo sapiens] gb|AAI01025.1| Synaptotagmin 10 [Homo sapiens] gb|AAP37477.1| synaptotagmin 10 [Homo sapiens] sp|Q6XYQ8|SYT10_HUMAN Synaptotagmin-10 (Synaptotagmin X) (SytX) 5.0E-55
blast NP_945343 synaptotagmin 10 [Homo sapiens] gb|AAI01025.1| Synaptotagmin 10 [Homo sapiens] gb|AAP37477.1| synaptotagmin 10 [Homo sapiens] sp|Q6XYQ8|SYT10_HUMAN Synaptotagmin-10 (Synaptotagmin X) (SytX) 6.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 9.4E-33
Pfam IPR000008 EMBL-EBI C2 domain 1.8E-31
Pfam IPR000008 EMBL-EBI C2 domain 9.4E-33
Pfam IPR000008 EMBL-EBI C2 domain 1.8E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503409.1 1 7-29
R12A1.2.1 1 7-29
R12A1.2.2 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:182682_AT
aattcacggtctacgatttcgatcggttcacccgccacggactcattggaaacatcataa
tgcgagatcttttcgaaaagtccgatctttacacgtggacagaatatacaatgcatattg
ttgcaagtcaaaacaagaacgattacggtgatttgttactttttttgacctactcgaatc
aagaggagaagctgtatatcaacgtgtcaaaagcttataatttaaggccaatggatataa
cgggtgcttcagatccatatgtgaaaatcgagcaaacttttaacgggaaacggttcaaat
cgaaaaaatcttcaacaaaacgagcgaatttgaatccagtgttccacgagacccttgtgt
ttgatatcccttcatctcaaattgcggaaaccaatattttaattcaggttatggactggg
atagaataggaaaagatgatcttcttggttgctgtattttgggcaaagatagtccgactg
ctgatggtaaaactcaatgggacaactgtcttaatgcggtgcccgaagaggggcagctga
aaaacaaaccagttggtcaatggcacagcctactctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTCACGGTCTACGATTTCGATCG 664 175 338 Antisense
CCCGCCACGGACTCATTGGAAACAT 130 257 368 Antisense
GAAAAGTCCGATCTTTACACGTGGA 664 351 412 Antisense
AACGGGTGCTTCAGATCCATATGTG 682 143 576 Antisense
TTGAATCCAGTGTTCCACGAGACCC 113 703 667 Antisense
ACGAGACCCTTGTGTTTGATATCCC 595 89 683 Antisense
GATATCCCTTCATCTCAAATTGCGG 602 423 700 Antisense
GATGATCTTCTTGGTTGCTGTATTT 241 411 772 Antisense
GATAGTCCGACTGCTGATGGTAAAA 525 423 805 Antisense
GGACAACTGTCTTAATGCGGTGCCC 591 525 837 Antisense
TTGGTCAATGGCACAGCCTACTCTC 317 709 890 Antisense
Sequence Source Affymetrix Proprietary Database