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[182675_at]

Description Y4C6B.3 /REP_DB=WormBase Gene ID /WP=CE21307 /TR=Q9UAZ8 /GB=AAD14766.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 92      6grp_total > 67      4grp_total > 03      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1180
1 2 3 4 range
A 282.3
(-2.2)
P 		124.6
(-3)
P 		144.5
(-2.9)
P 		1276.3
(3)
P 		1152
B 267.1
(-2)
P 		177.1
(-2.5)
P 		96.5
(-3)
P 		1113.9
(3)
P 		1017
C 273.4
(-2.3)
P 		149.1
(-3)
P 		206.5
(-2.7)
P 		1181.2
(3)
P 		1032
avr 274.3
(-2.3) 		150.3
(-3) 		149.2
(-3) 		1190.5
(3) 		1041

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 406
1 2 3 4 5 6 range
A 159
(-3)
P 		168
(-2.8)
P 		201
(-2.1)
P 		160.7
(-3)
P 		200.4
(-2.1)
P 		442.4
(3)
P 		283
B 160.4
(-2.6)
P 		169.3
(-2.4)
P 		131.1
(-3)
P 		142.8
(-2.8)
P 		231.7
(-1.5)
P 		535.1
(3)
P 		404
C 129.5
(-3)
P 		192
(-1.9)
P 		155.9
(-2.5)
P 		187.5
(-2)
P 		202.4
(-1.7)
P 		466.1
(3)
P 		337
avr 149.6
(-3) 		176.4
(-2.5) 		162.7
(-2.8) 		163.7
(-2.7) 		211.5
(-1.9) 		481.2
(3) 		332
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 215
1 2 3 4 range
A 102.8
(-0.2)
P 		27.1
(-3)
P 		37.8
(-2.6)
P 		186.8
(3)
P 		160
B 85.2
(-1.2)
P 		43.7
(-2.4)
P 		22.4
(-3)
P 		237.4
(3)
P 		215
C 90.5
(-1.2)
P 		42.9
(-3)
P 		72
(-1.9)
P 		199.2
(3)
P 		156
avr 92.8
(-1.1) 		37.9
(-3) 		44.1
(-2.8) 		207.8
(3) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 29.3
(-3)
P 		59.8
(-0.7)
P 		43.8
(-1.9)
P 		40.8
(-2.1)
P 		60.5
(-0.6)
P 		107.7
(3)
P 		78
B 55.8
(-2.1)
P 		56.4
(-2.1)
P 		31.8
(-3)
P 		37.8
(-2.8)
P 		94.2
(-0.8)
P 		199.6
(3)
P 		168
C 48
(-2.5)
P 		58.6
(-1.9)
P 		39.7
(-2.9)
P 		37.7
(-3)
P 		65.5
(-1.6)
P 		156
(3)
P 		118
avr 44.4
(-2.7) 		58.3
(-2) 		38.4
(-3) 		38.8
(-3) 		73.4
(-1.2) 		154.4
(3) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182675_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10765
Sequence Type Exemplar sequence
Representative Public ID Y4C6B.3 NCBI
Target Description Y4C6B.3 /REP_DB=WormBase Gene ID /WP=CE21307 /TR=Q9UAZ8 /GB=AAD14766.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068382(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068382 NCBI Caenorhabditis elegans Y4C6B.3 (Y4C6B.3) mRNA, complete cds. 11/11 None
GENEFINDER00000011105 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5332467:5336684:1 11/11 None
Y4C6B.3 ENSEMBL cdna:known chromosome:CEL140:IV:5333987:5336100:1 gene:Y4C6B.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011097 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5333992-5336106(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y4C6B.3
Entrez Gene 177313 Entrez gene
SwissProt Q9UAZ8 EMBL-EBI
WormBase CE21307 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD14766 Hypothetical protein Y4C6B.3 [Caenorhabditis elegans] ref|NP_500783.1| Y4C6B.3 [Caenorhabditis elegans] 0.0
blast CAE72728 Hypothetical protein CBG19966 [Caenorhabditis briggsae] 0.0
blast AAN73291 Hypothetical protein Y4C6B.4a [Caenorhabditis elegans] ref|NP_741396.1| Y4C6B.4a [Caenorhabditis elegans] 0.0
blast AAN73291 Hypothetical protein Y4C6B.4a [Caenorhabditis elegans] ref|NP_741396.1| Y4C6B.4a [Caenorhabditis elegans] 1.0E-180
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500783.1 10 20-39,46-68,73-95,107-129,139-158,201-223,238-260,272-294,298-320,333-355
Y4C6B.3 10 20-39,46-68,73-95,107-129,139-158,201-223,238-260,272-294,298-320,333-355
GENEFINDER00000011105 13 13-35,66-85,92-114,152-174,187-206,231-253,296-318,333-355,367-389,393-415,428-450,465-484,505-527
Sequence
Target Sequence 
>C. ELEGANS:182675_AT
gcattcacagaactttccacattgattttgatcgcaacttatgctccaatttatttcaga
aatgttctgaaatttgacataaaaatgattggattctatcttggtctcattctggcaatt
catcttccgttcagattcgtatgtgcgctattgagtgacaaaactcgatgtatatcagaa
atgtcaaaaatacacattttcaacacattatcagtcggctttgttggaatctcgtttatc
gcttttggacaaattccacacgaacataacttatcggcagtgatttgtttgggagtttta
gaatgttttaccagtttcagcagtggaggattttataaatgtggaacacttcatgcgagg
caattctcttcaattgtaatatctgcaattcaatttacaaagtgtattgctctattttcg
ggtccagctttggttgcatttttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATTCACAGAACTTTCCACATTGA 666 307 637 Antisense
GATTTTGATCGCAACTTATGCTCCA 692 429 660 Antisense
TTGGATTCTATCTTGGTCTCATTCT 76 709 725 Antisense
CATTCTGGCAATTCATCTTCCGTTC 347 215 744 Antisense
CTTCCGTTCAGATTCGTATGTGCGC 483 219 760 Antisense
TTTTCAACACATTATCAGTCGGCTT 112 675 833 Antisense
CAGTCGGCTTTGTTGGAATCTCGTT 699 207 848 Antisense
GAATCTCGTTTATCGCTTTTGGACA 642 327 863 Antisense
GTGGAACACTTCATGCGAGGCAATT 621 489 977 Antisense
GTATTGCTCTATTTTCGGGTCCAGC 275 453 1040 Antisense
GGGTCCAGCTTTGGTTGCATTTTTT 90 497 1056 Antisense
Sequence Source Affymetrix Proprietary Database