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[182668_s_at]

Description F53A3.2 /REP_DB=WormBase Gene ID /WP=CE28442 /TR=O17219 /GB=AAB70988.3 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 35      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 61.5
(-0.8)
A 		115.9
(3)
A 		29
(-3)
A 		79.5
(0.5)
A 		87
B 75.9
(-1)
A 		89.1
(1)
A 		63
(-3)
A 		101.7
(3)
A 		39
C 83.7
(0.5)
M 		57.8
(-3)
A 		102.1
(3)
A 		60.4
(-2.6)
A 		44
avr 73.7
(-0.6) 		87.6
(3) 		64.7
(-3) 		80.5
(1.1) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 25
(-3)
A 		61.3
(-2.2)
A 		89.1
(-1.5)
A 		121.4
(-0.8)
M 		169.8
(0.3)
A 		285.6
(3)
P 		261
B 32
(-2.9)
A 		43.5
(-2.7)
A 		28.9
(-3)
A 		84.2
(-1.8)
A 		203.4
(0.8)
P 		305.1
(3)
P 		276
C 107.6
(-1.2)
A 		42.1
(-2.6)
A 		60.9
(-2.2)
A 		22.7
(-3)
A 		241
(1.7)
P 		302.6
(3)
P 		280
avr 54.9
(-2.9) 		49
(-3) 		59.6
(-2.7) 		76.1
(-2.3) 		204.7
(0.8) 		297.8
(3) 		249
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 22.4
(2.1)
A 		25.1
(3)
A 		7.6
(-3)
A 		11.6
(-1.6)
A 		18
B 24.2
(3)
A 		22
(1.6)
A 		14.6
(-3)
A 		21.7
(1.4)
A 		10
C 27.7
(1.1)
M 		16.6
(-1.5)
A 		35.6
(3)
A 		10.2
(-3)
A 		25
avr 24.8
(3) 		21.2
(0.9) 		19.3
(-0.2) 		14.5
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 4.6
(-3)
A 		21.8
(-1.4)
A 		19.4
(-1.6)
A 		30.8
(-0.6)
M 		51.2
(1.3)
A 		69.5
(3)
P 		65
B 11.1
(-2.8)
A 		14.5
(-2.6)
A 		7
(-3)
A 		22.3
(-2.1)
A 		82.6
(1.2)
P 		113.8
(3)
P 		107
C 39.9
(-0.8)
A 		12.8
(-2.5)
A 		15.5
(-2.3)
A 		4.6
(-3)
A 		78
(1.6)
P 		101.3
(3)
P 		97
avr 18.5
(-2.7) 		16.4
(-2.8) 		14
(-3) 		19.2
(-2.6) 		70.6
(1.2) 		94.9
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182668_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8111
Sequence Type Exemplar sequence
Representative Public ID F53A3.2 NCBI
Target Description F53A3.2 /REP_DB=WormBase Gene ID /WP=CE28442 /TR=O17219 /GB=AAB70988.3 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065079(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065079 NCBI Caenorhabditis elegans POLH (DNA polymerase eta) homolog family member (polh-1) (polh-1) mRNA, complete cds. 11/11 None
SNAP00000027589 ENSEMBL cdna:SNAP chromosome:CEL140:III:1926604:1950219:-1 10/11 None
F53A3.2 ENSEMBL cdna:known chromosome:CEL140:III:1945109:1950246:-1 gene:F53A3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000027597 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1945108-1950246(-) 98.3 99.85
Public Domain and Genome References
Gene Title
Gene Symbol polh-1
Ensembl F53A3.2
Entrez Gene 175337 Entrez gene
SwissProt O17219 EMBL-EBI
WormBase CE28442 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:14851_AT UMUC-like DNA repair family protein at
ATH1-121501:249028_AT UMUC-like DNA repair family protein at
CANINE:1588441_AT similar to polymerase (DNA directed), eta cfa
CANINE_2:CFA.10259.1.S1_AT similar to polymerase (DNA directed), eta cfa
CANINE_2:CFAAFFX.3789.1.S1_S_AT similar to polymerase (DNA directed), eta cfa
DROSOPHILA_2:1626494_AT dm
DROSGENOME1:141627_AT dm
CHICKEN:GGA.7127.1.S1_S_AT polymerase (DNA directed), eta gga
CHICKEN:GGA.7127.2.S1_AT polymerase (DNA directed), eta gga
CHICKEN:GGAAFFX.21759.1.S1_AT polymerase (DNA directed), eta gga
CHICKEN:GGAAFFX.6516.1.S1_AT polymerase (DNA directed), eta gga
HG-U133B:222879_S_AT polymerase (DNA directed), eta hs
HG-U133_PLUS_2:222879_S_AT polymerase (DNA directed), eta hs
HG-U133_PLUS_2:219380_X_AT polymerase (DNA directed), eta hs
HG-U133A:219380_X_AT polymerase (DNA directed), eta hs
HG-FOCUS:219380_X_AT polymerase (DNA directed), eta hs
HG-U133A_2:219380_X_AT polymerase (DNA directed), eta hs
HU35KSUBA:HUM_ALU_AT polymerase (DNA directed), eta hs
HU35KSUBB:HUM_ALU_AT polymerase (DNA directed), eta hs
HU35KSUBC:HUM_ALU_AT polymerase (DNA directed), eta hs
HU35KSUBD:HUM_ALU_AT polymerase (DNA directed), eta hs
HUGENEFL:HUM_ALU_AT polymerase (DNA directed), eta hs
HC-G110:HUM_ALU_AT polymerase (DNA directed), eta hs
U133_X3P:G5457143_3P_A_AT polymerase (DNA directed), eta hs
U133_X3P:HS2.407274.1.A1_3P_S_AT polymerase (DNA directed), eta hs
U133_X3P:219380_3P_X_AT polymerase (DNA directed), eta hs
HG-U133_PLUS_2:1557701_S_AT polymerase (DNA directed), eta hs
HU35KSUBD:RC_AA425665_AT Polymerase (DNA directed), eta hs
U133_X3P:HS.155573.1.S1_3P_AT Polymerase (DNA directed), eta hs
HG-U133_PLUS_2:1557700_AT Polymerase (DNA directed), eta hs
HG-U133_PLUS_2:233852_AT Polymerase (DNA directed), eta hs
HG-U133B:233852_AT Polymerase (DNA directed), eta hs
HG-U95B:42167_AT Polymerase (DNA directed), eta hs
HG-U133_PLUS_2:1569341_AT Polymerase (DNA directed), eta hs
HG-U133_PLUS_2:237877_AT Polymerase (DNA directed), eta hs
HG-U133B:237877_AT Polymerase (DNA directed), eta hs
HG-U95D:74299_AT Polymerase (DNA directed), eta hs
U133_X3P:HS.311756.0.A1_3P_AT Polymerase (DNA directed), eta hs
MOE430A:1450075_AT polymerase (DNA directed), eta (RAD 30 related) mm
MOUSE430A_2:1450075_AT polymerase (DNA directed), eta (RAD 30 related) mm
MOUSE430_2:1450075_AT polymerase (DNA directed), eta (RAD 30 related) mm
MOE430A:1420988_AT polymerase (DNA directed), eta (RAD 30 related) mm
MOUSE430A_2:1420988_AT polymerase (DNA directed), eta (RAD 30 related) mm
MOUSE430_2:1420988_AT polymerase (DNA directed), eta (RAD 30 related) mm
RAE230B:1393823_AT polymerase (DNA directed), eta (RAD 30 related) (predicted) rn
RAT230_2:1393823_AT polymerase (DNA directed), eta (RAD 30 related) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6281 DNA repair inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB70988 Polh (dna polymerase eta) homolog protein 1 [Caenorhabditis elegans] ref|NP_497480.1| POLH (DNA polymerase eta) homolog family member (polh-1) [Caenorhabditis elegans] 0.0
blast CAE66466 Hypothetical protein CBG11743 [Caenorhabditis briggsae] 0.0
blast CAG32324 hypothetical protein [Gallus gallus] 3.0E-70
blast CAG32324 hypothetical protein [Gallus gallus] 4.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR001126 EMBL-EBI UMUC-like DNA-repair protein 6.8E-58
Pfam IPR001126 EMBL-EBI UMUC-like DNA-repair protein 2.0E-54
Sequence
Target Sequence 
>C. ELEGANS:182668_S_AT
taaaaatgaggactccggtccctggacaccgccaattctcaacatttcactatcagcgac
acgttttcaacccggaattcctgcacaaaatcgatcgattcacgaatggctttacgagaa
aaaaacgcgaaaatcgcgtcgaaaagcggcgatttacgatgaaaatgatggtcgagaaga
tgtgatttttgttgaaccgccggaaaaagtaccggaaaatgatcaaaaatccgagttttt
agtgaaaaaatcgaatttttttcagctgaaaaagccgaatccaacaccaaaatccactgg
aacagacggagatttcattgttttaggctcagattcggatgatgacgttgtacagccaaa
aactgatgataattggatttttgtcgatggaaagaaattttcaaaaatggcctggaggca
cctaccgcctgacattaaaaagcattaccaacatcgaatagcgttggaagaagctcgagc
gctgaaatcgaagactggtggctcacggaaaagagccggagctcacacggacaatgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAAATGAGGACTCCGGTCCCTGG 51 639 1482 Antisense
CGCCAATTCTCAACATTTCACTATC 363 257 1511 Antisense
ACTATCAGCGACACGTTTTCAACCC 83 103 1530 Antisense
TTTTCAACCCGGAATTCCTGCACAA 574 673 1545 Antisense
ATGTGATTTTTGTTGAACCGCCGGA 625 47 1661 Antisense
GCTGAAAAAGCCGAATCCAACACCA 89 297 1746 Antisense
TCATTGTTTTAGGCTCAGATTCGGA 241 617 1796 Antisense
TCGGATGATGACGTTGTACAGCCAA 586 605 1816 Antisense
AAAAATGGCCTGGAGGCACCTACCG 566 69 1884 Antisense
CACCTACCGCCTGACATTAAAAAGC 370 199 1900 Antisense
GCCGGAGCTCACACGGACAATGCTC 289 283 1996 Antisense
Sequence Source Affymetrix Proprietary Database