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[182665_at]

Description Y48C3A.8 /REP_DB=WormBase Gene ID /WP=CE22141 /TR=Q9NAG3 /GB=CAB55107.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_high_8_8 > 58      6grp_highest_nonorm > 90      6grp_total > 64      4grp_total > 60      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 218.1
(3)
P 		152.1
(-3)
P 		161.6
(-2.1)
P 		159.9
(-2.3)
P 		66
B 160.9
(-1.1)
P 		183.6
(0.4)
P 		221.9
(3)
P 		132.9
(-3)
P 		89
C 202.8
(3)
P 		198.7
(2.8)
P 		203.5
(3)
P 		82
(-3)
A 		122
avr 193.9
(2.8) 		178.1
(1.5) 		195.7
(3) 		124.9
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 96.6
(-1.2)
P 		89.2
(-1.7)
P 		158.1
(3)
P 		123.7
(0.7)
P 		69.6
(-3)
P 		121.6
(0.5)
P 		89
B 169
(3)
P 		96
(-2.7)
P 		151.7
(1.7)
P 		154.5
(1.9)
P 		91.9
(-3)
P 		155.2
(1.9)
P 		77
C 87.4
(-2.9)
P 		100.3
(-1.7)
P 		148.9
(2.9)
P 		149.7
(3)
P 		86.6
(-3)
P 		98.4
(-1.9)
P 		63
avr 117.7
(-0) 		95.2
(-1.9) 		152.9
(3) 		142.6
(2.1) 		82.7
(-3) 		125.1
(0.6) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 79.4
(3)
P 		33
(-2)
P 		42.2
(-1)
P 		23.4
(-3)
P 		56
B 51.3
(3)
P 		45.4
(1.4)
P 		51.4
(3)
P 		28.3
(-3)
P 		23
C 67.1
(2.6)
P 		57.1
(1.5)
P 		70.9
(3)
P 		13.8
(-3)
A 		57
avr 65.9
(3) 		45.2
(0.2) 		54.8
(1.5) 		21.8
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 17.8
(-3)
P 		31.7
(2)
P 		34.4
(3)
P 		31.4
(1.9)
P 		21
(-1.8)
P 		29.6
(1.3)
P 		17
B 58.8
(3)
P 		32
(-3)
P 		36.8
(-1.9)
P 		40.9
(-1)
P 		37.3
(-1.8)
P 		57.9
(2.8)
P 		27
C 32.4
(-0.3)
P 		30.6
(-1.4)
P 		37.9
(3)
P 		30.1
(-1.7)
P 		28
(-3)
P 		33
(0)
P 		10
avr 36.3
(0.9) 		31.4
(-1.6) 		36.4
(1) 		34.1
(-0.2) 		28.8
(-3) 		40.2
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182665_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3701
Sequence Type Exemplar sequence
Representative Public ID Y48C3A.8 NCBI
Target Description Y48C3A.8 /REP_DB=WormBase Gene ID /WP=CE22141 /TR=Q9NAG3 /GB=CAB55107.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064414(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064414 NCBI Caenorhabditis elegans Y48C3A.8 (Y48C3A.8) mRNA, complete cds. 11/11 None
SNAP00000006616 ENSEMBL cdna:SNAP chromosome:CEL140:II:13311496:13325577:-1 9/11 None
GENEFINDER00000006660 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13323342:13330421:-1 9/11 None
Y48C3A.8 ENSEMBL cdna:known chromosome:CEL140:II:13320937:13325577:-1 gene:Y48C3A.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13320933-13325574(-) 98.97 100.0
Public Domain and Genome References
Gene Title
Ensembl Y48C3A.8
Entrez Gene 174975 Entrez gene
SwissProt Q9NAG3 EMBL-EBI
WormBase CE22141 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55106 Hypothetical protein Y48C3A.7 [Caenorhabditis elegans] ref|NP_496814.1| Member of AAA family binding CED-4 family member (mac-1) [Caenorhabditis elegans] 0.0
blast T31590 hypothetical protein Y48C3A.h - Caenorhabditis elegans 0.0
blast AAF05624 cell survival CED-4-interacting protein MAC-1 [Caenorhabditis elegans] 0.0
blast CAB55107 Hypothetical protein Y48C3A.8 [Caenorhabditis elegans] ref|NP_496815.1| Y48C3A.8 [Caenorhabditis elegans] 3.0E-64
blast CAB60419 Hypothetical protein Y48C3A.9 [Caenorhabditis elegans] ref|NP_496816.1| Y48C3A.9 [Caenorhabditis elegans] 1.0E-56
blast T31593 hypothetical protein Y48C3A.k - Caenorhabditis elegans 7.0E-51
blast CAB55107 Hypothetical protein Y48C3A.8 [Caenorhabditis elegans] ref|NP_496815.1| Y48C3A.8 [Caenorhabditis elegans] 1.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 2.0E-65
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 5.2E-85
Pfam IPR007591 EMBL-EBI Single-stranded DNA-binding protein, SSDP 4.7E-16
Sequence
Target Sequence 
>C. ELEGANS:182665_AT
gtgttccacaggtggctacgacgtctgtaggatcagttggaacaccatcatcaatcggac
agcagctacatcagccgaagcaagagattacgacgaatggagaagaaataatgaagactg
aagctttgacaccgacgggtggaggtggaggtggaagtgttccaccaccaccaccggcgg
ccacagcagcagtgtcaatgaacggaggaggtccaggatcggcgccgggctccgcgcata
gtgtgaataataatgttaatccgggaacgccgggtagtaatccgttgagcaatccgatga
gtaatccaccattatcatctgggccaccgccgccgggtagtaatgatgcgtttgggaaga
atttaatgattttttttcctaaactaaccccaaaaatatcccagttttttccccaatttt
ctcagatttttacttatattccacccaactttaacttattaaatatccataccctactag
atacgtttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTCCACAGGTGGCTACGACGTC 503 481 350 Antisense
GAACACCATCATCAATCGGACAGCA 676 351 389 Antisense
CGGACAGCAGCTACATCAGCCGAAG 87 241 405 Antisense
AAGACTGAAGCTTTGACACCGACGG 627 151 463 Antisense
CGGCCACAGCAGCAGTGTCAATGAA 540 241 527 Antisense
AACGGAGGAGGTCCAGGATCGGCGC 577 139 550 Antisense
GGCTCCGCGCATAGTGTGAATAATA 394 537 577 Antisense
AATCCACCATTATCATCTGGGCCAC 323 167 652 Antisense
CACCGCCGCCGGGTAGTAATGATGC 427 201 674 Antisense
TATATTCCACCCAACTTTAACTTAT 257 661 784 Antisense
ATCCATACCCTACTAGATACGTTTT 208 39 814 Antisense
Sequence Source Affymetrix Proprietary Database