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[182656_at]

Description T10B5.5 /REP_DB=WormBase Gene ID /WP=CE28255 /TR=Q9TZS5 /GB=AAC19232.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 0      6grp_total > 16      4grp_total > 09      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4194
1 2 3 4 range
A 2019.1
(-3)
P 		2505.7
(-2.3)
P 		2836.5
(-1.8)
P 		6132.3
(3)
P 		4113
B 1938.7
(-3)
P 		2417.4
(-2.3)
P 		2870.3
(-1.6)
P 		6041.3
(3)
P 		4103
C 1958.2
(-3)
P 		2777.4
(-1.4)
P 		2724.3
(-1.5)
P 		5044.7
(3)
P 		3087
avr 1972
(-3) 		2566.8
(-2.1) 		2810.4
(-1.7) 		5739.4
(3) 		3767

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1468
1 2 3 4 5 6 range
A 2029.8
(2.6)
P 		2076.1
(3)
P 		1393.5
(-2.2)
P 		1437.5
(-1.8)
P 		1286
(-3)
P 		1538.2
(-1.1)
P 		790
B 1989.7
(3)
P 		1989.5
(3)
P 		1611.5
(-0.3)
P 		1311.5
(-3)
P 		1438.5
(-1.9)
P 		1350.4
(-2.7)
P 		678
C 2638.3
(3)
P 		1769.7
(-0.5)
P 		1808
(-0.4)
P 		1169.9
(-3)
P 		1425.9
(-2)
P 		1227.5
(-2.8)
P 		1468
avr 2219.3
(3) 		1945.1
(1.2) 		1604.3
(-1) 		1306.3
(-3) 		1383.5
(-2.5) 		1372
(-2.6) 		913
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 744
1 2 3 4 range
A 735.2
(0.2)
P 		543.9
(-3)
P 		741.5
(0.4)
P 		897.6
(3)
P 		354
B 618.2
(-2.8)
P 		597
(-3)
P 		665.1
(-2.4)
P 		1287.4
(3)
P 		690
C 648.2
(-3)
P 		798.5
(-0)
P 		949.5
(3)
P 		850.6
(1)
P 		301
avr 667.2
(-2.7) 		646.5
(-3) 		785.4
(-0.7) 		1011.9
(3) 		365

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 743
1 2 3 4 5 6 range
A 374.2
(-2)
P 		738.4
(3)
P 		303.5
(-3)
P 		364.6
(-2.2)
P 		388.1
(-1.8)
P 		374.5
(-2)
P 		435
B 692.3
(3)
P 		662.2
(2.5)
P 		391.3
(-2.2)
P 		347.4
(-3)
P 		584.5
(1.1)
P 		503.6
(-0.3)
P 		345
C 977.9
(3)
P 		540
(-0.5)
P 		460.2
(-1.2)
P 		235.3
(-3)
P 		461.4
(-1.2)
P 		410.9
(-1.6)
P 		743
avr 681.5
(3) 		646.9
(2.4) 		385
(-1.9) 		315.8
(-3) 		478
(-0.3) 		429.7
(-1.1) 		366
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182656_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15364
Sequence Type Exemplar sequence
Representative Public ID T10B5.5 NCBI
Target Description T10B5.5 /REP_DB=WormBase Gene ID /WP=CE28255 /TR=Q9TZS5 /GB=AAC19232.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071121(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071121 NCBI Caenorhabditis elegans T10B5.5a (T10B5.5) mRNA, complete cds. 10/11 None
T10B5.5b.2 ENSEMBL cdna:known chromosome:CEL140:V:1872072:1875322:1 gene:T10B5.5 10/11 None
T10B5.5b.1 ENSEMBL cdna:known chromosome:CEL140:V:1872074:1875322:1 gene:T10B5.5 10/11 None
T10B5.5a ENSEMBL cdna:known chromosome:CEL140:V:1872080:1875354:1 gene:T10B5.5 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182379 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178670 2 1 NM_071121 171922_x_at (11), 182656_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1872079-1875002(+) 100.0 100.0
Public Domain and Genome References
Ensembl T10B5.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC19232 Hypothetical protein T10B5.5a [Caenorhabditis elegans] ref|NP_503522.1| T10B5.5a [Caenorhabditis elegans] 0.0
blast CAE74146 Hypothetical protein CBG21817 [Caenorhabditis briggsae] 0.0
blast AAO25994 Hypothetical protein T10B5.5b [Caenorhabditis elegans] ref|NP_872179.1| T10B5.5b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 8.4E-117
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:182656_AT
cttcttcgcggtggagccgagcagttcatcgccgagaccgaacgatctcttcacgacgct
atcatgattgttcgtcgtgccaagaagaacgattcgatcgtcgccggaggaggtgccatc
gagatggagttgagccgattgattcgtgagcattcgaagggtattgagggcaaggatcaa
gctttctggatggcttatggacaggctttcgaggttttcgtcgtttttctcccgtttttt
aggttagttaaggcagatttcaagcttcgaagaaaccaaaaaatccatttttttcaccaa
aaacacccaaaaaaaaagctaatttccgaaatttttcagatcatcccacgccagctttgc
caaaacgccggactcgatgctctcgacgtgctgaacaagctgagacatcgtcatgctcaa
ggcgagaaatgggccggaatcgacatccatcgtgaagccgtcggagataatcttgtcgca
tgtgtctgggagccgagcatcgtgaagaggaacgccatcacagctgccaccgaagccgcg
tgcctcgttctttcgatcgatcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTTCGCGGTGGAGCCGAGCAGT 93 221 1123 Antisense
GTTCATCGCCGAGACCGAACGATCT 158 443 1146 Antisense
GCTATCATGATTGTTCGTCGTGCCA 425 303 1180 Antisense
TCGCCGGAGGAGGTGCCATCGAGAT 158 597 1223 Antisense
GACAGGCTTTCGAGGTTTTCGTCGT 229 349 1322 Antisense
GATGCTCTCGACGTGCTGAACAAGC 252 413 1498 Antisense
GAGACATCGTCATGCTCAAGGCGAG 450 393 1524 Antisense
AATGGGCCGGAATCGACATCCATCG 473 161 1550 Antisense
ACATCCATCGTGAAGCCGTCGGAGA 710 103 1565 Antisense
TCTTGTCGCATGTGTCTGGGAGCCG 297 617 1593 Antisense
TGCCTCGTTCTTTCGATCGATCAAA 309 585 1663 Antisense
Sequence Source Affymetrix Proprietary Database