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[182648_at]

Description C25G6.4 /REP_DB=WormBase Gene ID /WP=CE04085 /TR=Q18178 /GB=AAA82356.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 06      4grp_total > 13      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 205
(0.1)
P 		88.7
(-2.8)
A 		81.9
(-3)
A 		323.1
(3)
P 		241
B 169.9
(-0.6)
P 		84.1
(-2.7)
A 		73.3
(-3)
A 		316.3
(3)
P 		243
C 195.1
(0.2)
P 		125.2
(-1.3)
A 		41.7
(-3)
A 		333.3
(3)
P 		292
avr 190
(-0.1) 		99.3
(-2.2) 		65.6
(-3) 		324.2
(3) 		259

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 311.6
(3)
A 		157.3
(-1.2)
A 		152
(-1.3)
P 		111.7
(-2.4)
A 		121.2
(-2.1)
A 		88.8
(-3)
A 		223
B 205.6
(3)
P 		176.9
(1.8)
A 		104.7
(-1.3)
P 		77.4
(-2.4)
A 		95.9
(-1.7)
A 		64.4
(-3)
A 		141
C 223.3
(3)
P 		139.3
(-0.1)
A 		99.7
(-1.6)
A 		80.1
(-2.4)
A 		63.1
(-3)
A 		87.5
(-2.1)
A 		160
avr 246.8
(3) 		157.8
(-0.2) 		118.8
(-1.6) 		89.7
(-2.7) 		93.4
(-2.5) 		80.2
(-3) 		167
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 74.6
(3)
P 		19.2
(-3)
A 		21.4
(-2.8)
A 		47.3
(0)
P 		55
B 54.2
(1.4)
P 		20.8
(-2.5)
A 		17
(-3)
A 		67.4
(3)
P 		50
C 64.6
(3)
P 		36
(-0.4)
A 		14.5
(-3)
A 		56.2
(2)
P 		50
avr 64.5
(3) 		25.3
(-2) 		17.6
(-3) 		57
(2) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 57.4
(3)
A 		55.9
(2.7)
A 		33.1
(-1.1)
P 		28.3
(-1.9)
A 		36.6
(-0.5)
A 		21.6
(-3)
A 		36
B 71.5
(3)
P 		58.9
(1.5)
A 		25.4
(-2.4)
P 		20.5
(-3)
A 		39
(-0.8)
A 		24
(-2.6)
A 		51
C 82.8
(3)
P 		42.5
(-0.6)
A 		25.4
(-2.2)
A 		16.1
(-3)
A 		20.4
(-2.6)
A 		29.3
(-1.8)
A 		67
avr 70.6
(3) 		52.4
(0.8) 		28
(-2.2) 		21.6
(-3) 		32
(-1.7) 		25
(-2.6) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18601
Sequence Type Exemplar sequence
Representative Public ID C25G6.4 NCBI
Target Description C25G6.4 /REP_DB=WormBase Gene ID /WP=CE04085 /TR=Q18178 /GB=AAA82356.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075832
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000031324 ENSEMBL cdna:SNAP chromosome:CEL140:X:1247886:1248834:-1 None
GENEFINDER00000031333 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:1247886:1248834:-1 None
C25G6.4 ENSEMBL cdna:known chromosome:CEL140:X:1247828:1249833:-1 gene:C25G6.4 None
NM_075832 NCBI Caenorhabditis elegans C25G6.4 (C25G6.4) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1247885-1249499(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25G6.4
Entrez Gene 182920 Entrez gene
SwissProt Q18178 EMBL-EBI
WormBase CE37095 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82356 Hypothetical protein C25G6.4 [Caenorhabditis elegans] ref|NP_508233.2| C25G6.4 [Caenorhabditis elegans] 3.0E-89
blast AAA82356 Hypothetical protein C25G6.4 [Caenorhabditis elegans] ref|NP_508233.2| C25G6.4 [Caenorhabditis elegans] 1.0E-67
blast CAE63565 Hypothetical protein CBG08052 [Caenorhabditis briggsae] 3.0E-44
blast CAE63565 Hypothetical protein CBG08052 [Caenorhabditis briggsae] 2.0E-41
Sequence
Target Sequence 
>C. ELEGANS:182648_AT
gcgagtgcacttaaggcagacatctaggcaggtaggacagaattttcccccaagaacaca
tgcaaaaaagaaaaagtttagatctctcaaatttcgagacaaggaaacaggcgctgtagt
tctccacaaagcagtcgttggaatttgcaatgggctccattctcgagaacatgtgaatca
gtcggagtttgacaagcaggagcaagaatttagagtcaacagaaacttccgtgttgccgc
tgatgcttcatcaactgggccaagctacacccagaagcactcctggatagacggtttccg
gagcaatatcatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGAGTGCACTTAAGGCAGACATCT 112 291 15 Antisense
GCAGGTAGGACAGAATTTTCCCCCA 562 241 42 Antisense
GAATTTTCCCCCAAGAACACATGCA 285 325 54 Antisense
GGAAACAGGCGCTGTAGTTCTCCAC 642 525 117 Antisense
GTAGTTCTCCACAAAGCAGTCGTTG 298 455 130 Antisense
TTTGCAATGGGCTCCATTCTCGAGA 1 669 158 Antisense
GCCGCTGATGCTTCATCAACTGGGC 507 285 250 Antisense
TCATCAACTGGGCCAAGCTACACCC 670 615 262 Antisense
CTACACCCAGAAGCACTCCTGGATA 159 227 279 Antisense
GCACTCCTGGATAGACGGTTTCCGG 106 315 291 Antisense
GACGGTTTCCGGAGCAATATCATGC 693 375 304 Antisense
Sequence Source Affymetrix Proprietary Database