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[182625_at]

Description F10A3.12 /REP_DB=WormBase Gene ID /WP=CE15777 /TR=O45343 /GB=CAB07348.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 5
(-2.5)
A 		8.9
(-0.6)
A 		3.9
(-3)
A 		16.2
(3)
A 		12
B 4.9
(-3)
A 		5.7
(-2.9)
A 		5.6
(-2.9)
A 		44.8
(3)
A 		40
C 11
(-1.1)
A 		4
(-3)
A 		23.3
(2.1)
A 		26.6
(3)
A 		23
avr 7
(-2.8) 		6.2
(-3) 		10.9
(-1.8) 		29.2
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 7.2
(-1.4)
A 		12.8
(2.5)
A 		5.8
(-2.3)
A 		6.7
(-1.7)
A 		13.6
(3)
A 		4.8
(-3)
A 		9
B 3.5
(-2.7)
A 		7.6
(-1.6)
A 		3.2
(-2.7)
A 		4
(-2.5)
A 		25.9
(3)
A 		2.2
(-3)
A 		24
C 2.3
(-3)
A 		9.5
(-1.5)
A 		3.7
(-2.7)
A 		31.5
(3)
A 		5.6
(-2.3)
A 		3.4
(-2.8)
A 		29
avr 4.3
(-2.6) 		10
(0.4) 		4.2
(-2.6) 		14.1
(2.5) 		15
(3) 		3.5
(-3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.8
(0.4)
A 		1.9
(0.9)
A 		1
(-3)
A 		2.4
(3)
A 		1
B 1.6
(-2.8)
A 		1.4
(-2.9)
A 		1.3
(-3)
A 		9.5
(3)
A 		8
C 3.6
(-0.9)
A 		1.1
(-3)
A 		8.1
(3)
A 		4.5
(-0.1)
A 		7
avr 2.3
(-1.8) 		1.5
(-3) 		3.5
(0) 		5.5
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.3
(-2.8)
A 		4.5
(3)
A 		1.3
(-2.8)
A 		1.7
(-2.1)
A 		4.1
(2.3)
A 		1.2
(-3)
A 		3
B 1.2
(-2.8)
A 		2.5
(-1.9)
A 		0.8
(-3)
A 		1.1
(-2.8)
A 		10.5
(3)
A 		0.8
(-3)
A 		10
C 0.9
(-3)
A 		2.9
(-0.8)
A 		0.9
(-3)
A 		6.3
(3)
A 		1.8
(-2)
A 		1.1
(-2.8)
A 		5
avr 1.1
(-2.9) 		3.3
(0.1) 		1
(-3) 		3
(-0.3) 		5.5
(3) 		1
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182625_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12622
Sequence Type Exemplar sequence
Representative Public ID F10A3.12 NCBI
Target Description F10A3.12 /REP_DB=WormBase Gene ID /WP=CE15777 /TR=O45343 /GB=CAB07348.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074672(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074672 NCBI Caenorhabditis elegans F10A3.12 (F10A3.12) mRNA, complete cds. 11/11 None
SNAP00000000101 ENSEMBL cdna:SNAP chromosome:CEL140:V:16167412:16168056:1 11/11 None
GENEFINDER00000000106 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16167102:16168174:1 11/11 None
F10A3.12 ENSEMBL cdna:known chromosome:CEL140:V:16167000:16168056:1 gene:F10A3.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16166999-16168056(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F10A3.12
Entrez Gene 184281 Entrez gene
SwissProt O45343 EMBL-EBI
WormBase CE15777 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07348 Hypothetical protein F10A3.12 [Caenorhabditis elegans] ref|NP_507073.1| F10A3.12 [Caenorhabditis elegans] 1.0E-179
blast CAB07348 Hypothetical protein F10A3.12 [Caenorhabditis elegans] ref|NP_507073.1| F10A3.12 [Caenorhabditis elegans] 1.0E-156
blast CAB07349 Hypothetical protein F10A3.13 [Caenorhabditis elegans] ref|NP_507074.1| Seven TM Receptor family member (str-108) [Caenorhabditis elegans] 1.0E-134
blast CAB07349 Hypothetical protein F10A3.13 [Caenorhabditis elegans] ref|NP_507074.1| Seven TM Receptor family member (str-108) [Caenorhabditis elegans] 1.0E-130
blast CAB07350 Hypothetical protein F10A3.15 [Caenorhabditis elegans] ref|NP_507075.2| Seven TM Receptor family member (str-111) [Caenorhabditis elegans] 1.0E-127
blast CAB07348 Hypothetical protein F10A3.12 [Caenorhabditis elegans] ref|NP_507073.1| F10A3.12 [Caenorhabditis elegans] 1.0E-125
blast CAE46671 Hypothetical protein K05D4.9 [Caenorhabditis elegans] emb|CAE46675.1| Hypothetical protein K05D4.9 [Caenorhabditis elegans] ref|NP_001024031.1| K05D4.9 [Caenorhabditis elegans] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.2E-7
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 4.4E-26
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 4.4E-26
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 9.1E-5
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 4.4E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507073.1 7 10-32,45-67,77-99,120-142,190-212,241-263,273-295
F10A3.12 7 10-32,45-67,77-99,120-142,190-212,241-263,273-295
SNAP00000000101 4 29-51,99-121,150-172,182-204
GENEFINDER00000000106 6 12-31,58-80,100-122,170-192,221-243,253-275
Sequence
Target Sequence 
>C. ELEGANS:182625_AT
tcggtacgtgtcagtagttcggccatcggatattgaaatatttgccccaaaacgtttcca
tttgtgggctctcctactcatttcaaactttgccacatggtttttctgcttctactactt
aaatggaccatctgaaatgaaggatgaagaattgattccggaatttatggaagcttactg
tctacaaccaaatgaatatacctacacaggacctcactactactacacggaccattcaac
tggagaatggaaattccatatcccgtcatttatcgctgaaggatacacaggtggcactat
agttttaactataatttctgtagcatactttggtcaccaaacctaccaacatctctacaa
acttggaacaatgacaagtgtgaattatcgggaggttcagaagcaattattcaaaactct
agtggttcaaacaatattcccaagtatctttttatttttcccagtttcctgtttggtatt
attcccgctttttggaataagaatcggcgaagatgctaatttaataatgatctcattctc
catctacccatgtttcgaaccgctggtggcaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGTACGTGTCAGTAGTTCGGCCA 150 605 315 Antisense
TTGCCCCAAAACGTTTCCATTTGTG 443 695 356 Antisense
CTCTCCTACTCATTTCAAACTTTGC 545 231 383 Antisense
CTTTGCCACATGGTTTTTCTGCTTC 391 223 402 Antisense
GGAAGCTTACTGTCTACAACCAAAT 23 531 483 Antisense
TACTACTACACGGACCATTCAACTG 535 643 532 Antisense
TCCATATCCCGTCATTTATCGCTGA 666 589 569 Antisense
TCTGTAGCATACTTTGGTCACCAAA 586 607 631 Antisense
TCCTGTTTGGTATTATTCCCGCTTT 117 593 781 Antisense
ATAATGATCTCATTCTCCATCTACC 685 25 838 Antisense
ATGTTTCGAACCGCTGGTGGCAATA 383 47 864 Antisense
Sequence Source Affymetrix Proprietary Database