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[182621_at]

Description F35B12.3 /REP_DB=WormBase Gene ID /WP=CE05802 /TR=Q20013 /GB=CAA98466.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 13      6grp_total > 34      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3365
1 2 3 4 range
A 4273.9
(3)
P 		1803.4
(-1.4)
P 		920.5
(-3)
P 		3186.5
(1.1)
P 		3353
B 3682.3
(1.9)
P 		1757.6
(-1.6)
P 		963.1
(-3)
P 		4285
(3)
P 		3322
C 3860
(3)
P 		1662.2
(-1.6)
P 		1007.7
(-3)
P 		3243.3
(1.7)
P 		2852
avr 3938.7
(3) 		1741.1
(-1.4) 		963.8
(-3) 		3571.6
(2.3) 		2975

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2324
1 2 3 4 5 6 range
A 3094.8
(2.4)
P 		3074
(2.3)
P 		3302.3
(3)
P 		2434.2
(0.4)
P 		1324.9
(-3)
P 		1638
(-2)
P 		1977
B 3138
(2.5)
P 		3276.8
(3)
P 		3168.1
(2.6)
P 		1953.6
(-1.9)
P 		1658.6
(-3)
P 		1861.5
(-2.2)
P 		1618
C 3648.6
(3)
P 		2798.9
(0.5)
P 		3019.6
(1.1)
P 		2254.3
(-1.2)
P 		1641.2
(-3)
P 		1916.5
(-2.2)
P 		2007
avr 3293.8
(3) 		3049.9
(2.2) 		3163.3
(2.6) 		2214
(-0.7) 		1541.6
(-3) 		1805.3
(-2.1) 		1752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1333
1 2 3 4 range
A 1556.1
(3)
P 		391.5
(-2.3)
P 		240.6
(-3)
P 		466.4
(-2)
P 		1316
B 1174.3
(3)
P 		434.1
(-1.7)
P 		223.2
(-3)
P 		913.1
(1.4)
P 		951
C 1277.8
(3)
P 		477.9
(-2.2)
P 		351.2
(-3)
P 		546.9
(-1.7)
P 		927
avr 1336.1
(3) 		434.5
(-2.1) 		271.7
(-3) 		642.1
(-0.9) 		1064

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 954
1 2 3 4 5 6 range
A 570.5
(-1.5)
P 		1093.3
(3)
P 		719.2
(-0.2)
P 		617.4
(-1.1)
P 		399.8
(-3)
P 		398.8
(-3)
P 		695
B 1091.9
(3)
P 		1090.7
(3)
P 		769.3
(-0.4)
P 		517.4
(-3)
P 		673.9
(-1.4)
P 		694.1
(-1.2)
P 		575
C 1352.4
(3)
P 		854
(-0.3)
P 		768.5
(-0.9)
P 		453.3
(-3)
P 		531.1
(-2.5)
P 		641.6
(-1.7)
P 		899
avr 1004.9
(2.9) 		1012.7
(3) 		752.3
(-0.2) 		529.4
(-3) 		534.9
(-2.9) 		578.2
(-2.4) 		483
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182621_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13813
Sequence Type Exemplar sequence
Representative Public ID F35B12.3 NCBI
Target Description F35B12.3 /REP_DB=WormBase Gene ID /WP=CE05802 /TR=Q20013 /GB=CAA98466.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073541(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073541 NCBI Caenorhabditis elegans F35B12.3 (F35B12.3) mRNA, complete cds. 11/11 None
SNAP00000002149 ENSEMBL cdna:SNAP chromosome:CEL140:V:11608142:11609039:-1 11/11 None
GENEFINDER00000002170 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11607146:11609012:-1 11/11 None
F35B12.3 ENSEMBL cdna:known chromosome:CEL140:V:11605859:11609090:-1 gene:F35B12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11608141-11609012(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35B12.3
Entrez Gene 185258 Entrez gene
SwissProt Q20013 EMBL-EBI
WormBase CE31929 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98466 Hypothetical protein F35B12.3 [Caenorhabditis elegans] ref|NP_505942.2| F35B12.3 [Caenorhabditis elegans] 1.0E-82
blast CAA98466 Hypothetical protein F35B12.3 [Caenorhabditis elegans] ref|NP_505942.2| F35B12.3 [Caenorhabditis elegans] 3.0E-72
blast CAE75330 Hypothetical protein CBG23308 [Caenorhabditis briggsae] 1.0E-52
blast CAE75330 Hypothetical protein CBG23308 [Caenorhabditis briggsae] 3.0E-44
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000002149 1 10-29
Sequence
Target Sequence 
>C. ELEGANS:182621_AT
caatctcaatactatccatcatctggacagcaaggataccgcggtggttatcaaagtaat
ccaggaatgcagggaggataccagggaaatggccaagacagctatcaagacttccaaggt
tatcagggatatcaaagaggtcaaggaggtcaaagaggatatcaaggataccaaagtaat
caaagaagtcaaggaagttatccagaaagatatgattcatcaatgtattctccagtcatg
gatctaacaaactattatggatcccaaggaactccacaatattatggatacccatcatct
gcttcctatcaaatgactccatactatcaaaatcaaaaccaaggatatatgcaacagcaa
caatacaacacaccatcccaatcatcttggtcaccaatgtattatggcaatggatatcaa
tcacaacaatcccagcagcaacaatcttcaatgggaattcgtccatatcagagttcatgg
ggtcaaacacagggcacaaatacttggggaagtccatatcaattcacgaaggttgatgcc
caagtcactgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCTCAATACTATCCATCATCTG 664 181 112 Antisense
GGACAGCAAGGATACCGCGGTGGTT 623 519 136 Antisense
GACAGCTATCAAGACTTCCAAGGTT 335 367 208 Antisense
TTATGGATACCCATCATCTGCTTCC 521 681 393 Antisense
ATCTGCTTCCTATCAAATGACTCCA 86 35 408 Antisense
ATCCCAATCATCTTGGTCACCAATG 488 37 486 Antisense
GATATCAATCACAACAATCCCAGCA 101 427 524 Antisense
GGAATTCGTCCATATCAGAGTTCAT 100 535 565 Antisense
TCATGGGGTCAAACACAGGGCACAA 173 619 586 Antisense
GGGGAAGTCCATATCAATTCACGAA 468 493 617 Antisense
GAAGGTTGATGCCCAAGTCACTGAT 686 333 639 Antisense
Sequence Source Affymetrix Proprietary Database