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[182619_at]

Description R03G5.3 /REP_DB=WormBase Gene ID /WP=CE07400 /TR=Q21667 /GB=AAA96065.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 51.2
(-2)
M 		35.1
(-3)
A 		38.4
(-2.8)
A 		130.6
(3)
P 		96
B 30
(-1.8)
A 		19
(-3)
A 		55.3
(1)
A 		73.9
(3)
A 		55
C 83.7
(0.5)
P 		29.6
(-2)
A 		7.5
(-3)
A 		139.5
(3)
P 		132
avr 55
(-1.1) 		27.9
(-3) 		33.7
(-2.6) 		114.7
(3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 63.2
(2.6)
P 		37.2
(0.1)
A 		50.2
(1.3)
A 		11.1
(-2.4)
A 		67.4
(3)
A 		5.1
(-3)
A 		62
B 59.8
(3)
P 		57.9
(2.7)
A 		41.3
(-0.3)
A 		44.6
(0.3)
A 		26.5
(-3)
A 		31
(-2.2)
A 		33
C 38.4
(-2.3)
A 		73.2
(3)
P 		50.5
(-0.4)
A 		51.9
(-0.2)
A 		50.7
(-0.4)
M 		33.7
(-3)
A 		40
avr 53.8
(2.6) 		56.1
(3) 		47.3
(1.4) 		35.9
(-0.7) 		48.2
(1.6) 		23.3
(-3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 18.6
(2.7)
M 		7.6
(-3)
A 		10
(-1.7)
A 		19.1
(3)
P 		12
B 9.6
(-0.3)
A 		4.7
(-3)
A 		12.8
(1.4)
A 		15.7
(3)
A 		11
C 27.7
(3)
P 		8.5
(-1.6)
A 		2.6
(-3)
A 		23.5
(2)
P 		25
avr 18.6
(2.6) 		6.9
(-3) 		8.5
(-2.2) 		19.4
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 11.7
(0.3)
P 		13.2
(0.8)
A 		10.9
(0)
A 		2.8
(-2.5)
A 		20.3
(3)
A 		1.2
(-3)
A 		19
B 20.8
(3)
P 		19.3
(2.2)
A 		10
(-3)
A 		11.8
(-2)
A 		10.8
(-2.6)
A 		11.5
(-2.2)
A 		11
C 14.2
(-1.1)
A 		22.3
(3)
P 		12.9
(-1.7)
A 		10.4
(-3)
A 		16.4
(0)
M 		11.3
(-2.5)
A 		12
avr 15.6
(1.4) 		18.3
(3) 		11.3
(-1.1) 		8.3
(-2.8) 		15.8
(1.5) 		8
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182619_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16791
Sequence Type Exemplar sequence
Representative Public ID R03G5.3 NCBI
Target Description R03G5.3 /REP_DB=WormBase Gene ID /WP=CE07400 /TR=Q21667 /GB=AAA96065.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076919(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076919 NCBI Caenorhabditis elegans R03G5.3 (R03G5.3) mRNA, complete cds. 11/11 None
GENEFINDER00000013520 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7800384:7805521:1 9/11 None
R03G5.3 ENSEMBL cdna:known chromosome:CEL140:X:7800384:7805615:1 gene:R03G5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7800382-7805602(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R03G5.3
Entrez Gene 187541 Entrez gene
SwissProt Q21667 EMBL-EBI
WormBase CE34351 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4714 transmembrane receptor protein tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5006 epidermal growth factor receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA96065 Hypothetical protein R03G5.3 [Caenorhabditis elegans] ref|NP_509320.3| R03G5.3 [Caenorhabditis elegans] 0.0
blast CAA79559 Hypothetical protein F54G8.1 [Caenorhabditis elegans] ref|NP_499029.1| F54G8.1 [Caenorhabditis elegans] sp|Q03599|YMB1_CAEEL Hypothetical protein F54G8.1 in chromosome III 1.0E-22
blast CAE58062 Hypothetical protein CBG01141 [Caenorhabditis briggsae] 4.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 9.7E-32
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 3.7E-6
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 9.2E-8
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 9.2E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509320.3 12 376-398,405-427,458-480,493-512,532-554,571-593,1023-1045,1058-1080,1138-1157,1178-1200,1220-1242,1262-1284
R03G5.3 12 376-398,405-427,458-480,493-512,532-554,571-593,1023-1045,1058-1080,1138-1157,1178-1200,1220-1242,1262-1284
GENEFINDER00000013520 10 376-398,405-427,442-459,466-485,512-534,555-577,876-898,911-933,990-1009,1030-1052
Sequence
Target Sequence 
>C. ELEGANS:182619_AT
gaatcgtctcgctttacttatgtcgtgctctttatatcgtccattttatttgacaaatct
caattacgaaaaatacgcattgttagtgtcttgtatggtatttgttgtttctaatgtttg
gaacctgtcagtcatggaaaccttgcaaaataactttgcctatgtgttgtttcaaatgaa
ctggttaacattaattgtgaccccagtctctggaatagctgtgaaaatgattttgagaaa
gacatattccacgtataatgagctagcggtacatcataatcgattgtcatttggcattgt
ggaacttcattatttgtggttactcggatggtctgaatcaaatgttactcaaagtatctc
cttggctagaacttgttcggcaatgggaaaagtttttttactggtatggcggcattatga
tgagatggcactactcaatgttgatcatcattatgatagtctacctgcggagcacctttg
tcaaattccctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGTCTCGCTTTACTTATGTCG 521 329 3054 Antisense
TTATGTCGTGCTCTTTATATCGTCC 504 679 3071 Antisense
GGAACCTGTCAGTCATGGAAACCTT 504 529 3173 Antisense
ATAACTTTGCCTATGTGTTGTTTCA 492 25 3203 Antisense
TTAATTGTGACCCCAGTCTCTGGAA 580 683 3244 Antisense
ATTTGTGGTTACTCGGATGGTCTGA 199 13 3365 Antisense
GTTACTCAAAGTATCTCCTTGGCTA 477 441 3397 Antisense
TCTCCTTGGCTAGAACTTGTTCGGC 230 609 3410 Antisense
GAGATGGCACTACTCAATGTTGATC 618 395 3475 Antisense
TATGATAGTCTACCTGCGGAGCACC 706 655 3505 Antisense
GGAGCACCTTTGTCAAATTCCCTAT 1 521 3522 Antisense
Sequence Source Affymetrix Proprietary Database