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[182617_at]

Description T25B6.4 /REP_DB=WormBase Gene ID /WP=CE05014 /TR=Q22759 /GB=AAC48219.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 20      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 109.3
(-0.3)
A 		82.8
(-2.8)
A 		80.8
(-3)
A 		143.3
(3)
A 		63
B 141.6
(2.2)
M 		126.9
(1)
A 		76.7
(-3)
A 		152.1
(3)
P 		75
C 103.2
(-1.8)
A 		121.2
(-0.9)
A 		77.8
(-3)
A 		203.1
(3)
P 		125
avr 118
(-0.3) 		110.3
(-0.8) 		78.4
(-3) 		166.2
(3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 115.1
(3)
A 		75
(-1.4)
A 		88.6
(0.1)
A 		89.6
(0.2)
A 		86.8
(-0.1)
A 		60.1
(-3)
A 		55
B 102.6
(-0.3)
M 		140.1
(3)
A 		71.5
(-3)
A 		86.1
(-1.7)
A 		80.6
(-2.2)
A 		101.1
(-0.4)
A 		69
C 121.3
(0.6)
A 		151.4
(3)
P 		91.2
(-1.8)
A 		76.5
(-3)
A 		82.9
(-2.5)
A 		84.9
(-2.3)
A 		75
avr 113
(1.6) 		122.2
(3) 		83.8
(-2.7) 		84.1
(-2.7) 		83.4
(-2.8) 		82
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 39.8
(3)
A 		18
(-3)
A 		21.1
(-2.1)
A 		21
(-2.2)
A 		22
B 45.2
(3)
M 		31.3
(-0)
A 		17.8
(-3)
A 		32.4
(0.2)
P 		27
C 34.2
(2.5)
A 		34.9
(3)
A 		27.1
(-3)
A 		34.2
(2.5)
P 		8
avr 39.7
(3) 		28.1
(-0.9) 		22
(-3) 		29.2
(-0.6) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 21.2
(0.3)
A 		26.7
(3)
A 		19.3
(-0.7)
A 		22.7
(1)
A 		26.2
(2.8)
A 		14.6
(-3)
A 		12
B 35.7
(0.8)
M 		46.6
(3)
A 		17.4
(-3)
A 		22.8
(-1.9)
A 		32.8
(0.2)
A 		37.7
(1.2)
A 		29
C 45
(2.8)
A 		46.2
(3)
P 		23.2
(-1.5)
A 		15.4
(-3)
A 		26.8
(-0.8)
A 		28.4
(-0.5)
A 		31
avr 34
(1.2) 		39.8
(3) 		20
(-3) 		20.3
(-2.9) 		28.6
(-0.4) 		26.9
(-0.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182617_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16782
Sequence Type Exemplar sequence
Representative Public ID T25B6.4 NCBI
Target Description T25B6.4 /REP_DB=WormBase Gene ID /WP=CE05014 /TR=Q22759 /GB=AAC48219.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077122(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077122 NCBI Caenorhabditis elegans T25B6.4 (T25B6.4) mRNA, complete cds. 11/11 None
GENEFINDER00000006435 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9015666:9016601:1 11/11 None
SNAP00000006427 ENSEMBL cdna:SNAP chromosome:CEL140:X:9015672:9016678:1 9/11 None
T25B6.4 ENSEMBL cdna:known chromosome:CEL140:X:9015708:9016601:1 gene:T25B6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9015706-9016600(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25B6.4
Entrez Gene 188876 Entrez gene
SwissProt Q22759 EMBL-EBI
WormBase CE05014 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509523 T25B6.4 [Caenorhabditis elegans] gb|AAC48219.1| Hypothetical protein T25B6.4 [Caenorhabditis elegans] 1.0E-50
blast NP_509523 T25B6.4 [Caenorhabditis elegans] gb|AAC48219.1| Hypothetical protein T25B6.4 [Caenorhabditis elegans] 1.0E-29
blast CAE57305 Hypothetical protein CBG00224 [Caenorhabditis briggsae] 4.0E-24
blast CAE57305 Hypothetical protein CBG00224 [Caenorhabditis briggsae] 5.0E-23
blast CAE57305 Hypothetical protein CBG00224 [Caenorhabditis briggsae] 2.0E-14
Sequence
Target Sequence 
>C. ELEGANS:182617_AT
agattcgtcagtctccgtgacaacttgatgctgattctcgattttctcagcgaaatcggt
ggtccatctgaggagcagatcgacaactataatatcatgaagattaagaccaactttttc
atcgacgaaattgactttcatctccgtggagatgtcacctccgagcaactaatggagtac
cttggcgtatacgcaatcttctaccaccgcagccgtacggagctaatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATTCGTCAGTCTCCGTGACAACT 176 65 31 Antisense
CTTGATGCTGATTCTCGATTTTCTC 190 219 54 Antisense
TTCTCAGCGAAATCGGTGGTCCATC 486 683 74 Antisense
ATCGGTGGTCCATCTGAGGAGCAGA 484 35 85 Antisense
TAAGACCAACTTTTTCATCGACGAA 601 635 135 Antisense
GAAATTGACTTTCATCTCCGTGGAG 245 363 157 Antisense
CTCCGTGGAGATGTCACCTCCGAGC 654 233 172 Antisense
ATGTCACCTCCGAGCAACTAATGGA 702 47 182 Antisense
AATGGAGTACCTTGGCGTATACGCA 263 163 201 Antisense
TGGCGTATACGCAATCTTCTACCAC 708 543 213 Antisense
CACCGCAGCCGTACGGAGCTAATGA 114 201 235 Antisense
Sequence Source Affymetrix Proprietary Database