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[182614_at]

Description F39G3.8 /REP_DB=WormBase Gene ID /WP=CE10126 /TR=O16273 /GB=AAB65333.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 12      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 51      6grp_total > 23      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 781
1 2 3 4 range
A 863.6
(2.8)
P 		315.3
(-3)
P 		323.7
(-2.9)
P 		881.5
(3)
P 		566
B 799.3
(1.3)
P 		297.3
(-2.6)
P 		252
(-3)
P 		1010.4
(3)
P 		758
C 830.2
(2)
P 		229.9
(-3)
P 		363.6
(-1.9)
P 		952.6
(3)
P 		723
avr 831
(1.9) 		280.8
(-3) 		313.1
(-2.7) 		948.2
(3) 		667

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 550
1 2 3 4 5 6 range
A 784.2
(3)
P 		653.7
(1.6)
P 		567.3
(0.6)
P 		485.1
(-0.3)
P 		338.7
(-1.9)
P 		234.2
(-3)
P 		550
B 745.1
(2.8)
P 		758
(3)
P 		601.5
(1)
P 		526
(-0)
P 		429.6
(-1.3)
P 		299.9
(-3)
P 		458
C 546.6
(0.2)
P 		745
(2.6)
P 		779.3
(3)
P 		531
(-0)
P 		312.4
(-2.7)
P 		283.7
(-3)
P 		496
avr 692
(2.6) 		718.9
(3) 		649.4
(2.1) 		514
(0.2) 		360.2
(-1.8) 		272.6
(-3) 		446
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 314.4
(3)
P 		68.4
(-3)
P 		84.6
(-2.6)
P 		129
(-1.5)
P 		246
B 254.9
(3)
P 		73.4
(-2.5)
P 		58.4
(-3)
P 		215.3
(1.8)
P 		197
C 274.8
(3)
P 		66.1
(-3)
P 		126.7
(-1.3)
P 		160.6
(-0.3)
P 		209
avr 281.4
(3) 		69.3
(-3) 		89.9
(-2.4) 		168.3
(-0.2) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 144.6
(-0)
P 		232.5
(3)
P 		123.6
(-0.7)
P 		123
(-0.7)
P 		102.2
(-1.5)
P 		57
(-3)
P 		176
B 259.3
(3)
P 		252.3
(2.7)
P 		146.1
(-1.6)
P 		139.3
(-1.9)
P 		174.5
(-0.4)
P 		111.8
(-3)
P 		148
C 202.6
(1.9)
P 		227.3
(3)
P 		198.3
(1.7)
P 		106.8
(-2.5)
P 		101.1
(-2.7)
P 		95
(-3)
P 		132
avr 202.2
(1.6) 		237.4
(3) 		156
(-0.3) 		123
(-1.6) 		125.9
(-1.5) 		87.9
(-3) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182614_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15706
Sequence Type Exemplar sequence
Representative Public ID F39G3.8 NCBI
Target Description F39G3.8 /REP_DB=WormBase Gene ID /WP=CE10126 /TR=O16273 /GB=AAB65333.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071870(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071870 NCBI Caenorhabditis elegans TransformIng Growth factor beta family member (tig-2) (tig-2) mRNA, complete cds. 11/11 None
SNAP00000029881 ENSEMBL cdna:SNAP chromosome:CEL140:V:4726610:4729934:-1 11/11 None
GENEFINDER00000029891 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4726610:4729934:-1 11/11 None
F39G3.8 ENSEMBL cdna:known chromosome:CEL140:V:4726543:4729936:-1 gene:F39G3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4726609-4729934(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tig-2
Ensembl F39G3.8
Entrez Gene 178864 Entrez gene
SwissProt O16273 EMBL-EBI
WormBase CE10126 Wormbase
Functional Annotations
Ortholog
ID Title Organism
MOE430A:1449873_AT bone morphogenetic protein 8a mm
MOUSE430A_2:1449873_AT bone morphogenetic protein 8a mm
MOUSE430_2:1449873_AT bone morphogenetic protein 8a mm
MU11KSUBA:M97017_S_AT bone morphogenetic protein 8a mm
MG-U74AV2:92982_AT bone morphogenetic protein 8a mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65333 Transforming growth factor beta family protein 2 [Caenorhabditis elegans] ref|NP_504271.1| TransformIng Growth factor beta family member (tig-2) [Caenorhabditis elegans] 0.0
blast CAE64179 Hypothetical protein CBG08804 [Caenorhabditis briggsae] 1.0E-145
blast AAF47075 CG5562-PA [Drosophila melanogaster] ref|NP_477340.1| CG5562-PA [Drosophila melanogaster] sp|P27091|60A_DROME 60A protein precursor (Glass bottom boat protein) gb|AAA28307.1| 60A protein gb|AAA28306.1| 'precursor TGF-B-like protein from 60A gene'; Potential glycosylation sites are located at positions 421-429, 1117-1125, and 1153-1161 9.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR001839 EMBL-EBI Transforming growth factor beta 1.2E-39
Sequence
Target Sequence 
>C. ELEGANS:182614_AT
ggacaccaatcttcctggagacacagttctcgtgtcatctgatgatccgacagtggtcac
tgatgtcactacaatggtcgatagatggtcacacttgcaactctcaacactaccaattgt
gacggctagagcttcaacaaatgatgagcttaaaatcgaggcatttctggtgatcgcgtt
gaaagacgaagacgcaggtccaccaaaaaaaagatcccgtagatccgcctccacaactcc
aatctccgcacctcccatgagacaaaaagtaaaacgaagtgaatccgcttactttgaaaa
acccaacgaaaatgaacggtgccaacgtaaaggtctttatgtcgactttgacattctcgg
ctggaaacaatgggttatcgcaccagaagggttcagtgcattttattgctccggtgattg
ctctgcaccattttcaaaagaaatgaatgccacgtcacacgctattgtacagtcaactct
gcacagagtacgcccgaattcgacgactccggcgaaatgtgccccgtcgagtttgggatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACACCAATCTTCCTGGAGACACA 574 525 486 Antisense
TAGATGGTCACACTTGCAACTCTCA 388 653 567 Antisense
GCAACTCTCAACACTACCAATTGTG 681 317 582 Antisense
AGGCATTTCTGGTGATCGCGTTGAA 63 55 644 Antisense
TCGACTTTGACATTCTCGGCTGGAA 438 599 827 Antisense
TTTATTGCTCCGGTGATTGCTCTGC 53 675 887 Antisense
GATTGCTCTGCACCATTTTCAAAAG 654 433 901 Antisense
GCCACGTCACACGCTATTGTACAGT 666 279 934 Antisense
TGCACAGAGTACGCCCGAATTCGAC 63 577 965 Antisense
TCGACGACTCCGGCGAAATGTGCCC 510 599 985 Antisense
AATGTGCCCCGTCGAGTTTGGGATC 248 161 1001 Antisense
Sequence Source Affymetrix Proprietary Database