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[182610_at]

Description Y60A3A.5 /REP_DB=WormBase Gene ID /WP=CE24529 /TR=Q9U1Z9 /GB=CAB60392.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 10      6grp_cgc > 64      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 31.4
(-2)
A 		54.8
(-1.2)
A 		3.8
(-3)
A 		173.5
(3)
A 		170
B 45.9
(-2.8)
A 		41.3
(-3)
A 		67.1
(-1.7)
A 		161.7
(3)
P 		120
C 40.6
(-2.3)
A 		58.1
(-1.6)
A 		23.3
(-3)
A 		171.3
(3)
A 		148
avr 39.3
(-2.7) 		51.4
(-2.1) 		31.4
(-3) 		168.8
(3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 20.4
(-0.9)
A 		7
(-3)
A 		41.1
(2.5)
A 		28.6
(0.5)
A 		44.4
(3)
A 		13.7
(-1.9)
A 		37
B 4.9
(-3)
A 		32.6
(1.9)
A 		38.5
(3)
A 		33.8
(2.2)
A 		32.6
(1.9)
A 		32.9
(2)
A 		34
C 24.6
(-1.4)
A 		67.7
(3)
A 		22.4
(-1.6)
A 		9.1
(-3)
A 		16.9
(-2.2)
A 		24.5
(-1.4)
A 		59
avr 16.6
(-3) 		35.8
(3) 		34
(2.4) 		23.8
(-0.8) 		31.3
(1.6) 		23.7
(-0.8) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 11.4
(-0.4)
A 		11.9
(-0.3)
A 		1
(-3)
A 		25.4
(3)
A 		24
B 14.6
(-1.9)
A 		10.2
(-3)
A 		15.5
(-1.7)
A 		34.5
(3)
P 		24
C 13.4
(-1.5)
A 		16.7
(-0.5)
A 		8.1
(-3)
A 		28.9
(3)
A 		21
avr 13.1
(-1.6) 		12.9
(-1.7) 		8.2
(-3) 		29.6
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 3.8
(-2.3)
A 		2.5
(-3)
A 		8.9
(0.5)
A 		7.3
(-0.4)
A 		13.4
(3)
A 		3.3
(-2.6)
A 		11
B 1.7
(-3)
A 		10.9
(1.8)
A 		9.3
(1)
A 		9
(0.8)
A 		13.2
(3)
A 		12.3
(2.5)
A 		12
C 9.1
(-0.7)
A 		20.7
(3)
A 		5.7
(-1.8)
A 		1.8
(-3)
A 		5.5
(-1.8)
A 		8.2
(-1)
A 		19
avr 4.9
(-3) 		11.4
(3) 		8
(-0.1) 		6
(-2) 		10.7
(2.4) 		7.9
(-0.2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182610_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14603
Sequence Type Exemplar sequence
Representative Public ID Y60A3A.5 NCBI
Target Description Y60A3A.5 /REP_DB=WormBase Gene ID /WP=CE24529 /TR=Q9U1Z9 /GB=CAB60392.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075464(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075464 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-171) (srh-171) mRNA, complete cds. 11/11 None
GENEFINDER00000032820 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19969003:19973060:1 9/11 None
Y60A3A.5 ENSEMBL cdna:known chromosome:CEL140:V:19963697:19970556:1 gene:Y60A3A.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032809 2/11 Cross Hyb Matching Probes None
SNAP00000032812 7/11 Cross Hyb Matching Probes None
GENEFINDER00000032847 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19963696-19970556(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-171
Ensembl Y60A3A.5
Entrez Gene 190433 Entrez gene
SwissProt Q9U1Z9 EMBL-EBI
WormBase CE24529 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60393 Hypothetical protein Y60A3A.4 [Caenorhabditis elegans] ref|NP_507866.2| Serpentine Receptor, class H family member (srh-173) [Caenorhabditis elegans] 1.0E-169
blast CAB60392 Hypothetical protein Y60A3A.5 [Caenorhabditis elegans] ref|NP_507865.1| Serpentine Receptor, class H family member (srh-171) [Caenorhabditis elegans] 1.0E-160
blast CAB60394 Hypothetical protein Y60A3A.6 [Caenorhabditis elegans] ref|NP_507864.2| Serpentine Receptor, class H family member (srh-172) [Caenorhabditis elegans] 1.0E-122
blast CAB04365 Hypothetical protein F40D4.1 [Caenorhabditis elegans] ref|NP_507348.1| Serpentine Receptor, class H family member (srh-174) [Caenorhabditis elegans] 1.0E-112
blast CAB60394 Hypothetical protein Y60A3A.6 [Caenorhabditis elegans] ref|NP_507864.2| Serpentine Receptor, class H family member (srh-172) [Caenorhabditis elegans] 1.0E-107
blast CAB04365 Hypothetical protein F40D4.1 [Caenorhabditis elegans] ref|NP_507348.1| Serpentine Receptor, class H family member (srh-174) [Caenorhabditis elegans] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.9E-82
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR004875 EMBL-EBI CENP-B protein 4.5E-7
Pfam IPR004875 EMBL-EBI CENP-B protein 1.1E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507865.1 7 17-39,52-74,94-116,136-158,192-214,238-260,275-294
Y60A3A.5 7 17-39,52-74,94-116,136-158,192-214,238-260,275-294
GENEFINDER00000032820 10 204-226,250-272,285-307,337-359,372-394,414-436,457-479,513-535,555-577,592-614
Sequence
Target Sequence 
>C. ELEGANS:182610_AT
tgaaaatcgctatcaccaactcatcaatagatcttctggaggcaggagttggaagcgaat
attatatgtgttgtttcactacgcgttatctgcaacatttatagctccgtcgtttttgaa
tattccaagtgtagatcaaggaaaagtacatacttttgagaaaatcccttgcatccccag
agacataccctcccgtcctggctactttgtcctggcaactgacaacctactcgtctgcct
ttgcatttctatcatgctcttgttcgtcggcgttcaatgggcttttttcgtggcgtccat
attccgctacctatccaacaccgtatcccattcccaaaccacaactcgtctccaacgtca
attcttcattgcaatgtgcattcaactgttaatcccgttggcagtaatcgtttttccggc
ggcgtttgtagtttttgcaataagatatgactattataatcaagccgccaacaacattgc
gatggctgctattgccactcatggagtttttaccacactgaccatgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAATCGCTATCACCAACTCATC 651 571 360 Antisense
AGCTCCGTCGTTTTTGAATATTCCA 244 81 462 Antisense
TCGTCTGCCTTTGCATTTCTATCAT 482 605 590 Antisense
CATTTCTATCATGCTCTTGTTCGTC 292 215 603 Antisense
GTCGGCGTTCAATGGGCTTTTTTCG 99 473 625 Antisense
TTTTTTCGTGGCGTCCATATTCCGC 468 675 642 Antisense
TGTGCATTCAACTGTTAATCCCGTT 160 549 734 Antisense
GTTGGCAGTAATCGTTTTTCCGGCG 440 255 756 Antisense
TCCGGCGGCGTTTGTAGTTTTTGCA 255 591 774 Antisense
GATGGCTGCTATTGCCACTCATGGA 87 407 840 Antisense
GAGTTTTTACCACACTGACCATGAT 498 397 863 Antisense
Sequence Source Affymetrix Proprietary Database