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[182608_s_at]

Description H12D21.3 /REP_DB=WormBase Gene ID /WP=CE15225 /TR=Q23304 /GB=CAB07424.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 0      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 18
(-3)
A 		18.8
(-2.9)
A 		36.6
(0.4)
A 		50.4
(3)
P 		32
B 28.9
(1.8)
A 		4.2
(-3)
A 		34.8
(3)
A 		22.2
(0.5)
A 		31
C 5.7
(-3)
A 		19.2
(-1.8)
A 		12.6
(-2.4)
A 		72.6
(3)
A 		67
avr 17.5
(-2.4) 		14.1
(-3) 		28
(-0.6) 		48.4
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 20.2
(-0.4)
A 		4.9
(-3)
A 		38
(2.6)
A 		13.4
(-1.6)
A 		40.6
(3)
A 		37.9
(2.5)
P 		36
B 8.2
(-2.2)
A 		18.4
(-0.3)
A 		4.1
(-3)
A 		34.2
(2.7)
A 		35.9
(3)
M 		28.3
(1.6)
A 		32
C 2.4
(-3)
A 		49.6
(3)
A 		19.3
(-0.9)
A 		4.4
(-2.7)
A 		23.4
(-0.3)
A 		31.4
(0.7)
A 		47
avr 10.3
(-3) 		24.3
(0.7) 		20.5
(-0.3) 		17.3
(-1.2) 		33.3
(3) 		32.5
(2.8) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 6.6
(-0.3)
A 		4.1
(-3)
A 		9.6
(3)
A 		7.4
(0.6)
P 		6
B 9.2
(3)
A 		1
(-3)
A 		8.1
(2.2)
A 		4.7
(-0.3)
A 		8
C 1.9
(-3)
A 		5.5
(-0.9)
A 		4.4
(-1.5)
A 		12.2
(3)
A 		10
avr 5.9
(0.1) 		3.5
(-3) 		7.4
(2.1) 		8.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 3.7
(-1.9)
A 		1.8
(-3)
A 		8.3
(0.7)
A 		3.4
(-2.1)
A 		12.3
(3)
A 		9.2
(1.2)
P 		11
B 2.9
(-2.2)
A 		6.1
(-0.8)
A 		1
(-3)
A 		9.1
(0.6)
A 		14.6
(3)
M 		10.5
(1.2)
A 		14
C 0.9
(-3)
A 		15.1
(3)
A 		4.9
(-1.3)
A 		0.9
(-3)
A 		7.6
(-0.2)
A 		10.5
(1.1)
A 		14
avr 2.5
(-3) 		7.7
(0.5) 		4.7
(-1.5) 		4.5
(-1.7) 		11.5
(3) 		10.1
(2.1) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182608_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13802
Sequence Type Exemplar sequence
Representative Public ID H12D21.3 NCBI
Target Description H12D21.3 /REP_DB=WormBase Gene ID /WP=CE15225 /TR=Q23304 /GB=CAB07424.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074267(11), NM_074264(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074267 NCBI Caenorhabditis elegans H12D21.3 (H12D21.3) mRNA, complete cds. 11/11 None
NM_074264 NCBI Caenorhabditis elegans ZC412.8 (ZC412.8) mRNA, complete cds. 11/11 None
SNAP00000018097 ENSEMBL cdna:SNAP chromosome:CEL140:V:14882118:14882369:-1 11/11 None
GENEFINDER00000018101 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14882118:14882369:-1 11/11 None
SNAP00000026960 ENSEMBL cdna:SNAP chromosome:CEL140:V:14888486:14888737:-1 11/11 None
GENEFINDER00000026973 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14888486:14888737:-1 11/11 None
ZC412.8 ENSEMBL cdna:known chromosome:CEL140:V:14882118:14882369:-1 gene:ZC412.8 11/11 None
H12D21.3 ENSEMBL cdna:known chromosome:CEL140:V:14888486:14888737:-1 gene:H12D21.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14888485-14888737(-) 100.0 100.0
chrV:14882117-14882369(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl ZC412.8
H12D21.3
Entrez Gene 186727 Entrez gene
191163 Entrez gene
SwissProt Q23304 EMBL-EBI
WormBase CE15225 Wormbase
CE15225 Wormbase
Sequence
Target Sequence 
>C. ELEGANS:182608_S_AT
atgttttcttcaaagctagctattgttcttcttatcgttgcttttgttgtggcttctgct
caaaataataaggaaggtggacaacaaaatggtggagagaacaagaatggaggaaaagca
acaactgcatcgaatggttctggaggtgctccaaatggacaaggaaatcaaaaaatagct
ggaactgatggaaacagtgggaatggaaaatctggacaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTTTCTTCAAAGCTAGCTATTG 413 47 13 Antisense
AGCTAGCTATTGTTCTTCTTATCGT 434 79 26 Antisense
TATTGTTCTTCTTATCGTTGCTTTT 586 665 33 Antisense
TCTTCTTATCGTTGCTTTTGTTGTG 358 617 39 Antisense
ATCGTTGCTTTTGTTGTGGCTTCTG 675 35 46 Antisense
TTGTTGTGGCTTCTGCTCAAAATAA 488 705 56 Antisense
AGGTGGACAACAAAATGGTGGAGAG 210 59 87 Antisense
GCAACAACTGCATCGAATGGTTCTG 385 319 130 Antisense
ATCGAATGGTTCTGGAGGTGCTCCA 482 35 141 Antisense
GGAGGTGCTCCAAATGGACAAGGAA 195 517 154 Antisense
GTGGGAATGGAAAATCTGGACAATC 357 489 209 Antisense
Sequence Source Affymetrix Proprietary Database