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[182605_s_at]

Description B0454.9 /REP_DB=WormBase Gene ID /WP=CE07759 /TR=O17173 /GB=AAB70946.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 70      6grp_total > 25      4grp_total > 03      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 952
1 2 3 4 range
A 409.2
(-2.4)
P 		352.4
(-2.7)
P 		311.9
(-3)
P 		1219.8
(3)
P 		908
B 423.1
(-1.9)
P 		271.1
(-3)
P 		344
(-2.5)
P 		1117.3
(3)
P 		846
C 411.6
(-1.6)
P 		268
(-3)
P 		318.8
(-2.5)
P 		892.8
(3)
P 		625
avr 414.6
(-2.1) 		297.2
(-3) 		324.9
(-2.8) 		1076.6
(3) 		779

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 387
(3)
P 		379.9
(2.7)
P 		309.2
(-0.3)
P 		247.5
(-3)
P 		286.3
(-1.3)
P 		290.2
(-1.2)
P 		140
B 328.2
(1.9)
P 		356
(3)
P 		351.8
(2.8)
P 		246.8
(-1.5)
P 		210.6
(-3)
P 		232
(-2.1)
P 		145
C 309.4
(3)
P 		283.4
(1.2)
P 		257
(-0.6)
P 		276.3
(0.7)
P 		221.9
(-3)
P 		257.6
(-0.6)
P 		88
avr 341.5
(3) 		339.8
(2.9) 		306
(0.9) 		256.9
(-2) 		239.6
(-3) 		259.9
(-1.8) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 149
(1.3)
P 		76.5
(-3)
P 		81.5
(-2.7)
P 		178.6
(3)
P 		102
B 134.9
(-0.6)
P 		66.9
(-3)
P 		79.7
(-2.6)
P 		238.1
(3)
P 		171
C 136.3
(1.8)
P 		77
(-3)
P 		111.1
(-0.2)
P 		150.5
(3)
P 		74
avr 140.1
(0.5) 		73.5
(-3) 		90.8
(-2.1) 		189.1
(3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 71.3
(-2.3)
P 		135.1
(3)
P 		67.3
(-2.6)
P 		62.8
(-3)
P 		86.4
(-1)
P 		70.7
(-2.3)
P 		72
B 114.2
(2.5)
P 		118.5
(3)
P 		85.4
(-0.7)
P 		65.4
(-3)
P 		85.6
(-0.7)
P 		86.5
(-0.6)
P 		53
C 114.7
(3)
P 		86.5
(0.1)
P 		65.4
(-2)
P 		55.6
(-3)
P 		71.8
(-1.4)
P 		86.2
(0.1)
P 		59
avr 100.1
(1.5) 		113.4
(3) 		72.7
(-1.7) 		61.3
(-3) 		81.3
(-0.7) 		81.1
(-0.7) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182605_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4450
Sequence Type Exemplar sequence
Representative Public ID B0454.9 NCBI
Target Description B0454.9 /REP_DB=WormBase Gene ID /WP=CE07759 /TR=O17173 /GB=AAB70946.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062034(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062034 NCBI Caenorhabditis elegans B0454.9 (B0454.9) mRNA, complete cds. 11/11 None
SNAP00000034456 ENSEMBL cdna:SNAP chromosome:CEL140:II:3052647:3054311:-1 11/11 None
B0454.9 ENSEMBL cdna:known chromosome:CEL140:II:3052647:3054316:-1 gene:B0454.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000034466 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3052645-3054310(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0454.9
Entrez Gene 173651 Entrez gene
SwissProt O17173 EMBL-EBI
WormBase CE07759 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70946 Hypothetical protein B0454.9 [Caenorhabditis elegans] ref|NP_494435.1| B0454.9 [Caenorhabditis elegans] 0.0
blast CAA85315 Hypothetical protein C06C3.8 [Caenorhabditis elegans] ref|NP_496001.2| C06C3.8 [Caenorhabditis elegans] 3.0E-60
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 5.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 6.0E-17
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 2.5E-5
Sequence
Target Sequence 
>C. ELEGANS:182605_S_AT
gttcgtccggttctatcgagaagtctctgaaggttgggaggatcggatgagaaagacgaa
gcaacttgagaaaagggagccagctgggggtgtcggaatcgtaggagacgacgagaattc
gatggatgttgaggaggagccgtatgagaatccggatattatttgggatgatgtcgctga
cccagagagccccacaacctcgatgagctctaccctcacctctacccagggaaccttcac
cgattatcatcccgaggaaccctaccacaacccggagccgccactgatccacccgacgct
cgagtatgccgccatgaagaccgcttcccttcagcgaaaccagcagaacaagcacgtgat
ggaggttcggcacggcagtgagactcgcacggtggtagtgatgcctacagagcctccgag
acagcaattgaatgctccagactttgcggaagaaatgctccaagtcacgtaccaagcgac
acggattgctcgagagcagccggaacgtgtgaagctgctccgcaaggcgcttttcgatac
agta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCGTCCGGTTCTATCGAGAAGTC 288 441 441 Antisense
CCAGCTGGGGGTGTCGGAATCGTAG 224 249 520 Antisense
TACCCAGGGAACCTTCACCGATTAT 53 649 663 Antisense
ACCGATTATCATCCCGAGGAACCCT 3 87 679 Antisense
TTCCCTTCAGCGAAACCAGCAGAAC 65 697 765 Antisense
GAGGTTCGGCACGGCAGTGAGACTC 170 403 802 Antisense
GACTCGCACGGTGGTAGTGATGCCT 435 373 822 Antisense
CCTACAGAGCCTCCGAGACAGCAAT 655 267 844 Antisense
GAATGCTCCAGACTTTGCGGAAGAA 402 329 870 Antisense
TGCTCCAAGTCACGTACCAAGCGAC 686 581 896 Antisense
CCGCAAGGCGCTTTTCGATACAGTA 298 263 960 Antisense
Sequence Source Affymetrix Proprietary Database