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[182574_at]

Description Y54E5B.2 /REP_DB=WormBase Gene ID /WP=CE19226 /TR=Q9XWF9 /GB=CAA21713.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 75      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 73      6grp_total > 75      4grp_total > 47      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 328
1 2 3 4 range
A 682.4
(3)
P 		461.7
(-2.8)
P 		453.2
(-3)
P 		479.3
(-2.3)
P 		229
B 709.1
(3)
P 		456.6
(-1.6)
P 		454.3
(-1.7)
P 		381
(-3)
P 		328
C 695.7
(3)
P 		386.9
(-3)
P 		475.4
(-1.3)
P 		441.3
(-1.9)
P 		309
avr 695.7
(3) 		435.1
(-3) 		461
(-2.4) 		433.9
(-3) 		262

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 335
1 2 3 4 5 6 range
A 481.3
(-1.9)
P 		460.2
(-3)
P 		534.5
(0.7)
P 		580.2
(3)
P 		569.3
(2.5)
P 		574.1
(2.7)
P 		120
B 491.6
(-3)
P 		509.5
(-2.4)
P 		591.3
(0.2)
P 		583.5
(-0)
P 		558.6
(-0.8)
P 		677.9
(3)
P 		186
C 433.7
(-2.4)
P 		553.5
(-0.3)
P 		521.2
(-0.9)
P 		722.2
(2.7)
P 		402.5
(-3)
P 		737.6
(3)
P 		335
avr 468.9
(-3) 		507.7
(-1.8) 		549
(-0.5) 		628.6
(1.9) 		510.1
(-1.7) 		663.2
(3) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 248.5
(3)
P 		100.2
(-2)
P 		118.5
(-1.4)
P 		70.2
(-3)
P 		178
B 226.1
(3)
P 		112.8
(-1.7)
P 		105.3
(-2)
P 		81.2
(-3)
P 		145
C 230.3
(3)
P 		111.2
(-1.6)
P 		165.7
(0.5)
P 		74.4
(-3)
P 		156
avr 235
(3) 		108.1
(-1.8) 		129.8
(-1) 		75.3
(-3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 88.7
(-3)
P 		163.7
(2.4)
P 		116.4
(-1)
P 		147.1
(1.2)
P 		171.8
(3)
P 		139.8
(0.7)
P 		83
B 171.1
(-1.5)
P 		169.6
(-1.6)
P 		143.6
(-3)
P 		154.6
(-2.4)
P 		226.9
(1.6)
P 		252.8
(3)
P 		109
C 160.7
(-1.4)
P 		168.9
(-1)
P 		132.7
(-2.9)
P 		145.2
(-2.2)
P 		130.2
(-3)
P 		246.9
(3)
P 		117
avr 140.2
(-2.3) 		167.4
(-0.3) 		130.9
(-3) 		149
(-1.7) 		176.3
(0.3) 		213.2
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182574_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.552
Sequence Type Exemplar sequence
Representative Public ID Y54E5B.2 NCBI
Target Description Y54E5B.2 /REP_DB=WormBase Gene ID /WP=CE19226 /TR=Q9XWF9 /GB=CAA21713.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061183(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061183 NCBI Caenorhabditis elegans Y54E5B.2 (Y54E5B.2) mRNA, complete cds. 11/11 None
SNAP00000016371 ENSEMBL cdna:SNAP chromosome:CEL140:I:14823883:14832797:-1 11/11 None
GENEFINDER00000016377 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14823883:14828340:-1 11/11 None
Y54E5B.2 ENSEMBL cdna:known chromosome:CEL140:I:14823828:14828340:-1 gene:Y54E5B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14823813-14828271(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E5B.2
Entrez Gene 173352 Entrez gene
SwissProt Q9XWF9 EMBL-EBI
WormBase CE19226 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21713 Hypothetical protein Y54E5B.2 [Caenorhabditis elegans] ref|NP_493584.1| Y54E5B.2 [Caenorhabditis elegans] 0.0
blast CAE72525 Hypothetical protein CBG19705 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.011
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.072
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.02
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.011
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.072
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.02
Sequence
Target Sequence 
>C. ELEGANS:182574_AT
cgtattttcacttcgccatgtgtctcgaccagacgaaggtcgcgttgttgctgttaaaac
gtgtcgaattgctcaaaaaacctatttcatcgcctcatattcatctggaacgattgaggt
ctttggtggaatatcactcgaatccgtgttttcgattgaaattgaagattctttgggaat
tgctgcgaaaatggattattcgaaatcgcagctcgttgttggaaccagtggcagttatat
tcttacttacgatttgctggaagatggagttctgagggagaaacaacgagtgaaactgcc
ggatcgaagtggtgtaacctccatctcatacatcccggaagacgactccacaattatcgg
atgtgattccggacatgtgtataaatatcacacatctacaatcacaaagatccattctca
tctatcgactgttgttggaattgttgtaactgatcgcaatcgaattcacaccgtttcgtt
ggactgtgcagtag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTATTTTCACTTCGCCATGTGTCT 394 247 1971 Antisense
GCCATGTGTCTCGACCAGACGAAGG 287 281 1985 Antisense
GACGAAGGTCGCGTTGTTGCTGTTA 694 379 2002 Antisense
ATTTCATCGCCTCATATTCATCTGG 147 15 2054 Antisense
GAATATCACTCGAATCCGTGTTTTC 482 325 2099 Antisense
GAAATCGCAGCTCGTTGTTGGAACC 630 361 2172 Antisense
GAAGTGGTGTAACCTCCATCTCATA 650 335 2276 Antisense
CCATCTCATACATCCCGGAAGACGA 506 271 2291 Antisense
ATCCATTCTCATCTATCGACTGTTG 624 39 2380 Antisense
ATCGCAATCGAATTCACACCGTTTC 193 37 2423 Antisense
ACCGTTTCGTTGGACTGTGCAGTAG 340 85 2440 Antisense
Sequence Source Affymetrix Proprietary Database