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[182571_at]

Description Y59A8B.2 /REP_DB=WormBase Gene ID /WP=CE26210 /TR=Q9GRZ4 /GB=CAC14405.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 60      6grp_total > 05      4grp_total > 59      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 570
1 2 3 4 range
A 319
(-1.2)
P 		401.4
(-0.3)
P 		714.1
(3)
P 		143.7
(-3)
P 		570
B 379.3
(-0.1)
P 		550.3
(3)
P 		471.8
(1.6)
P 		216.4
(-3)
P 		334
C 555.4
(1.8)
P 		653.7
(3)
P 		528.2
(1.5)
P 		160.5
(-3)
P 		493
avr 417.9
(0.7) 		535.1
(2.5) 		571.4
(3) 		173.5
(-3) 		398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 430
1 2 3 4 5 6 range
A 403.5
(0.7)
P 		245.8
(-2.2)
P 		250.5
(-2.1)
P 		202.6
(-3)
P 		527.7
(3)
P 		227.4
(-2.5)
P 		325
B 449.3
(-0.9)
P 		615.8
(3)
P 		394.7
(-2.2)
P 		508.9
(0.5)
P 		378.1
(-2.6)
P 		362.6
(-3)
P 		253
C 632.7
(3)
P 		431.1
(-0.1)
P 		395.6
(-0.7)
P 		328.9
(-1.7)
P 		365.5
(-1.2)
P 		247.8
(-3)
P 		385
avr 495.2
(3) 		430.9
(1.2) 		346.9
(-1.1) 		346.8
(-1.1) 		423.8
(1) 		279.3
(-3) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 116.1
(0.4)
P 		87.1
(-0.6)
P 		186.7
(3)
P 		21
(-3)
P 		166
B 121
(2)
P 		135.9
(3)
P 		109.3
(1.2)
P 		46.1
(-3)
P 		90
C 183.8
(2.8)
P 		187.9
(3)
P 		184.1
(2.9)
P 		27.1
(-3)
P 		161
avr 140.3
(2.1) 		137
(1.9) 		160
(3) 		31.4
(-3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 74.4
(-1.7)
P 		87.4
(-1)
P 		54.6
(-2.8)
P 		51.4
(-3)
P 		159.2
(3)
P 		55.4
(-2.8)
P 		108
B 156.3
(0.3)
P 		205
(3)
P 		95.8
(-3)
P 		134.8
(-0.9)
P 		153.6
(0.2)
P 		135.2
(-0.8)
P 		109
C 234.5
(3)
P 		131.6
(-0.7)
P 		100.7
(-1.8)
P 		66.1
(-3)
P 		118.3
(-1.1)
P 		82.9
(-2.4)
P 		168
avr 155.1
(3) 		141.3
(1.8) 		83.7
(-3) 		84.1
(-3) 		143.7
(2) 		91.2
(-2.4) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182571_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13357
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.2 NCBI
Target Description Y59A8B.2 /REP_DB=WormBase Gene ID /WP=CE26210 /TR=Q9GRZ4 /GB=CAC14405.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075113(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075113 NCBI Caenorhabditis elegans Y59A8B.2 (Y59A8B.2) mRNA, complete cds. 11/11 A 
SNAP00000028688 ENSEMBL cdna:SNAP chromosome:CEL140:V:17932197:17936631:1 11/11 None
Y59A8B.2 ENSEMBL cdna:known chromosome:CEL140:V:17932237:17936631:1 gene:Y59A8B.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028723 7/11 Cross Hyb Matching Probes None
GENEFINDER00000028724 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17932236-17936631(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y59A8B.2
Entrez Gene 3564997 Entrez gene
SwissProt Q9GRZ4 EMBL-EBI
WormBase CE26210 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC14405 Hypothetical protein Y59A8B.2 [Caenorhabditis elegans] ref|NP_507514.1| Y59A8B.2 [Caenorhabditis elegans] 0.0
blast CAC14405 Hypothetical protein Y59A8B.2 [Caenorhabditis elegans] ref|NP_507514.1| Y59A8B.2 [Caenorhabditis elegans] 1.0E-178
blast CAE61651 Hypothetical protein CBG05585 [Caenorhabditis briggsae] 1.0E-170
blast CAE61651 Hypothetical protein CBG05585 [Caenorhabditis briggsae] 1.0E-143
blast AAH43910 Usp8-prov protein [Xenopus laevis] 4.0E-54
blast AAH43910 Usp8-prov protein [Xenopus laevis] 1.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.4E-95
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 2.1E-67
Sequence
Target Sequence 
>C. ELEGANS:182571_AT
tatcaattctcgttatcgccggttaatcggcttcttgggagcattaccgtgtcagaaatc
agatgcctgacgtgcggagcttcttcggcgactttcgaggagaatacgataatttctgtg
gaaataccatccaattcgtcgtgctcactggatatgtgccttcgatcacacttttcgcaa
actaaattggacggagattctcgttggaattgtccaaaatgcaaggagccacgtgcaagt
acacggacatcaaaactgtggcaaccaccaccggttatgatcatccatctgaagcgtttc
gcgctgttcaacggggatttcgagaaaaacacggccgccgtgacatttgagaccgcccga
ttcgacgttcgaccgtatttacatgagatggcgcctgccgagaagcccgtctacaagctt
ta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCAATTCTCGTTATCGCCGGTTA 132 653 496 Antisense
GGCTTCTTGGGAGCATTACCGTGTC 68 537 524 Antisense
CGTGCTCACTGGATATGTGCCTTCG 565 245 635 Antisense
GCCTTCGATCACACTTTTCGCAAAC 578 281 653 Antisense
CAAAACTGTGGCAACCACCACCGGT 5 191 746 Antisense
ACCACCGGTTATGATCATCCATCTG 522 85 762 Antisense
CATCCATCTGAAGCGTTTCGCGCTG 656 209 777 Antisense
TTTCGCGCTGTTCAACGGGGATTTC 621 669 792 Antisense
ATTCGACGTTCGACCGTATTTACAT 136 9 855 Antisense
TACATGAGATGGCGCCTGCCGAGAA 638 643 875 Antisense
CCGAGAAGCCCGTCTACAAGCTTTA 429 263 893 Antisense
Sequence Source Affymetrix Proprietary Database