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[182557_at]

Description W03D2.2 /REP_DB=WormBase Gene ID /WP=CE14508 /TR=O02121 /GB=AAC48256.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 26.6
(2.9)
A 		7.7
(-3)
A 		26.9
(3)
A 		9.5
(-2.4)
A 		19
B 1.6
(-3)
A 		3.7
(-2.7)
A 		3.1
(-2.8)
A 		46
(3)
A 		44
C 0.9
(-3)
A 		23
(1.9)
A 		2.1
(-2.7)
A 		28
(3)
A 		27
avr 9.7
(-3) 		11.5
(-2.4) 		10.7
(-2.7) 		27.8
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 25.2
(2.6)
A 		26.8
(3)
A 		10.3
(-1.3)
A 		5.3
(-2.6)
A 		15.5
(0.1)
A 		3.7
(-3)
A 		23
B 3.1
(-2.7)
A 		14.6
(2)
A 		5.7
(-1.6)
A 		17.1
(3)
A 		5.7
(-1.6)
A 		2.3
(-3)
A 		15
C 5.1
(-2.2)
A 		26.4
(2.1)
A 		11
(-1)
A 		31.2
(3)
A 		2.3
(-2.7)
A 		0.8
(-3)
A 		30
avr 11.1
(-0.4) 		22.6
(3) 		9
(-1) 		17.9
(1.6) 		7.8
(-1.4) 		2.3
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 9.7
(3)
A 		1.7
(-2.8)
A 		7
(1)
A 		1.4
(-3)
A 		8
B 0.5
(-3)
A 		0.9
(-2.7)
A 		0.7
(-2.9)
A 		9.8
(3)
A 		9
C 0.3
(-3)
A 		6.6
(3)
A 		0.7
(-2.6)
A 		4.7
(1.2)
A 		6
avr 3.5
(-1.3) 		3.1
(-2.3) 		2.8
(-3) 		5.3
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 4.6
(-0.4)
A 		9.5
(3)
A 		2.2
(-2.1)
A 		1.3
(-2.7)
A 		4.7
(-0.3)
A 		0.9
(-3)
A 		9
B 1.1
(-2.6)
A 		4.9
(3)
A 		1.4
(-2.1)
A 		4.5
(2.4)
A 		2.3
(-0.8)
A 		0.8
(-3)
A 		4
C 1.9
(-1.8)
A 		8
(3)
A 		2.8
(-1.1)
A 		6.3
(1.7)
A 		0.7
(-2.7)
A 		0.3
(-3)
A 		8
avr 2.5
(-1.4) 		7.5
(3) 		2.1
(-1.8) 		4
(-0.1) 		2.6
(-1.3) 		0.7
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182557_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11245
Sequence Type Exemplar sequence
Representative Public ID W03D2.2 NCBI
Target Description W03D2.2 /REP_DB=WormBase Gene ID /WP=CE14508 /TR=O02121 /GB=AAC48256.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068072(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068072 NCBI Caenorhabditis elegans W03D2.2 (W03D2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000027047 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4065898:4069541:1 11/11 None
W03D2.2 ENSEMBL cdna:known chromosome:CEL140:IV:4068773:4069541:1 gene:W03D2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027043 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4068778-4069547(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03D2.2
Entrez Gene 189151 Entrez gene
SwissProt O02121 EMBL-EBI
WormBase CE14508 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48262 Seven tm receptor protein 122 [Caenorhabditis elegans] ref|NP_500472.1| Seven TM Receptor family member (str-122) [Caenorhabditis elegans] 1.0E-174
blast AAC48256 Hypothetical protein W03D2.2 [Caenorhabditis elegans] ref|NP_500473.1| W03D2.2 [Caenorhabditis elegans] 1.0E-140
blast AAC48256 Hypothetical protein W03D2.2 [Caenorhabditis elegans] ref|NP_500473.1| W03D2.2 [Caenorhabditis elegans] 1.0E-139
blast CAE66760 Hypothetical protein CBG12114 [Caenorhabditis briggsae] 3.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.0E-4
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.9E-81
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.0E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027047 6 7-29,90-112,133-152,200-222,254-276,291-313
Sequence
Target Sequence 
>C. ELEGANS:182557_AT
aaaaaacacttcttcgtgcaatcgaaactatgaagattaaagatttaatatgtttctcgt
ttacatcgaatttcgcaaagaatatagtgggagctttgaatctaaaactttacgatataa
agtgcatgatgggaggtgacgcacaccacctattgtttaatatttgtaaaaacattatat
acgtaacgatagactttctgtctgaaaattatttaatcagatccaacagacctcccgatg
ttgtatgcagacttccaggaataaatgcaaaagctttcttaaatcatattcttgaaactt
ttcattatcctacgattgactatttctcattttctctgaatacacgtgatggtcaatgga
tttataaagttctcaatggaatgcaaatccagaaaatgggaatagacgatgtttacacgg
actgtgccgaaactgacaaaaacgctataataaagttatcccaactttctcacaacatta
aacttaataatactcatcgaggcgatgctcaatttggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAAACACTTCTTCGTGCAATCGA 12 131 146 Antisense
GTTTCTCGTTTACATCGAATTTCGC 179 449 197 Antisense
TGACGCACACCACCTATTGTTTAAT 705 569 282 Antisense
TTTAATCAGATCCAACAGACCTCCC 162 673 357 Antisense
ACCTCCCGATGTTGTATGCAGACTT 107 89 375 Antisense
AACTTTTCATTATCCTACGATTGAC 574 137 441 Antisense
GATTGACTATTTCTCATTTTCTCTG 43 435 459 Antisense
TTTTCTCTGAATACACGTGATGGTC 673 675 475 Antisense
GACGATGTTTACACGGACTGTGCCG 313 377 550 Antisense
TATCCCAACTTTCTCACAACATTAA 594 657 602 Antisense
CATCGAGGCGATGCTCAATTTGGAG 51 213 640 Antisense
Sequence Source Affymetrix Proprietary Database