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[182554_at]

Description ZK218.11 /REP_DB=WormBase Gene ID /WP=CE16709 /TR=O46004 /GB=CAB04990.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 41      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 42.6
(-3)
A 		124.3
(1.1)
A 		162.1
(3)
P 		150.6
(2.4)
P 		120
B 68.6
(-3)
A 		122.8
(0.3)
M 		166.5
(3)
P 		113
(-0.3)
M 		98
C 79.4
(-3)
A 		184.5
(3)
P 		109.5
(-1.3)
A 		113.9
(-1)
A 		105
avr 63.5
(-3) 		143.9
(2.8) 		146
(3) 		125.8
(1.5) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 87.8
(3)
M 		56.1
(0.1)
A 		77.8
(2.1)
P 		45.5
(-0.9)
A 		56.6
(0.2)
A 		22
(-3)
A 		66
B 48.6
(-2.6)
A 		50.3
(-2.3)
A 		78.9
(3)
P 		46.7
(-3)
A 		48.5
(-2.7)
A 		48.9
(-2.6)
A 		32
C 95.7
(3)
M 		46.9
(-1.7)
A 		61.9
(-0.3)
A 		33.4
(-3)
A 		82
(1.7)
A 		50.5
(-1.4)
P 		62
avr 77.4
(3) 		51.1
(-1.3) 		72.9
(2.3) 		41.9
(-2.8) 		62.4
(0.6) 		40.5
(-3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 15.5
(-3)
A 		27
(-0.4)
A 		42.4
(3)
P 		22.1
(-1.5)
P 		27
B 21.9
(-3)
A 		30.3
(0)
M 		38.6
(3)
P 		24.1
(-2.2)
M 		17
C 26.3
(-1.7)
A 		53.1
(3)
P 		38.2
(0.4)
A 		19.2
(-3)
A 		34
avr 21.2
(-3) 		36.8
(2.1) 		39.7
(3) 		21.8
(-2.8) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 16.2
(1.4)
M 		20
(3)
A 		16.9
(1.7)
P 		11.5
(-0.5)
A 		17.1
(1.8)
A 		5.4
(-3)
A 		15
B 16.9
(0.7)
A 		16.7
(0.5)
A 		19.2
(2.6)
P 		12.4
(-3)
A 		19.7
(3)
A 		18.2
(1.8)
A 		7
C 35.5
(3)
M 		14.3
(-1.4)
A 		15.7
(-1.1)
A 		6.7
(-3)
A 		26.5
(1.1)
A 		16.9
(-0.9)
P 		29
avr 22.9
(3) 		17
(0.2) 		17.3
(0.4) 		10.2
(-3) 		21.1
(2.1) 		13.5
(-1.4) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182554_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14230
Sequence Type Exemplar sequence
Representative Public ID ZK218.11 NCBI
Target Description ZK218.11 /REP_DB=WormBase Gene ID /WP=CE16709 /TR=O46004 /GB=CAB04990.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074935(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074935 NCBI Caenorhabditis elegans ZK218.11 (ZK218.11) mRNA, complete cds. 11/11 None
SNAP00000041066 ENSEMBL cdna:SNAP chromosome:CEL140:V:17110108:17111035:-1 11/11 None
GENEFINDER00000041073 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17110108:17110978:-1 11/11 None
ZK218.11 ENSEMBL cdna:known chromosome:CEL140:V:17110108:17111035:-1 gene:ZK218.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17110107-17111035(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.11
Entrez Gene 191249 Entrez gene
SwissProt O46004 EMBL-EBI
WormBase CE16709 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04990 Hypothetical protein ZK218.11 [Caenorhabditis elegans] ref|NP_507336.1| ZK218.11 [Caenorhabditis elegans] 1.0E-150
blast CAB04990 Hypothetical protein ZK218.11 [Caenorhabditis elegans] ref|NP_507336.1| ZK218.11 [Caenorhabditis elegans] 1.0E-140
blast CAB04987 Hypothetical protein ZK218.7 [Caenorhabditis elegans] ref|NP_507335.1| ZK218.7 [Caenorhabditis elegans] 1.0E-103
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 3.0E-95
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 4.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 8.4E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.5E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 8.4E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.5E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507336.1 1 12-34
ZK218.11 1 12-34
SNAP00000041066 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:182554_AT
tgacactatctatattgccacaccagcagtgcaaccagtaccaggagctagcactgctcg
cccattgaaatgctacgctgcagcagcaaccgatccggtgaatccagatcttgtcaatgc
tgcagtcagcacttgcccaaagacttgtggattttgctgtcagagctctgactacaactg
ccccaatgttagattcccacgtctcaactgtgacacaatccttccatctcaatgcaaaga
tcaacaatggcgtgtcatcattgcccaagattgtccatccgcgtgtggattctgcaatca
aggaggatgtgttgatgctatcccccaatgcccgaacgacctctccatttgcaataacgt
cggaatgcaagactttgtcaatctgaactgccaaaagacgtgtggaagatgtccatcaac
cacaactccacgcaccggaggagttacagttccagggggaacatgtaccacctacaacgc
tgactcaagccgtacctgtgctgcttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACACTATCTATATTGCCACACCA 689 571 234 Antisense
ACCAGCAGTGCAACCAGTACCAGGA 178 87 255 Antisense
AGTACCAGGAGCTAGCACTGCTCGC 105 61 270 Antisense
GCTACGCTGCAGCAGCAACCGATCC 491 301 305 Antisense
AATGCTGCAGTCAGCACTTGCCCAA 132 167 349 Antisense
GTCAGAGCTCTGACTACAACTGCCC 591 465 392 Antisense
CAACTGCCCCAATGTTAGATTCCCA 661 177 408 Antisense
ACAATCCTTCCATCTCAATGCAAAG 403 113 448 Antisense
AGATTGTCCATCCGCGTGTGGATTC 414 65 501 Antisense
ACGACCTCTCCATTTGCAATAACGT 276 93 569 Antisense
CTCAAGCCGTACCTGTGCTGCTTGG 496 227 717 Antisense
Sequence Source Affymetrix Proprietary Database