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[182553_at]

Description C54D10.3 /REP_DB=WormBase Gene ID /WP=CE05499 /TR=Q18827 /GB=CAA99799.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 50      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 06      6grp_total > 51      4grp_total > 52      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6723
1 2 3 4 range
A 7368.6
(2.5)
P 		7958.5
(3)
P 		6920.3
(2)
P 		1489.7
(-3)
P 		6469
B 7431.2
(2.9)
P 		7572.5
(3)
P 		7395.4
(2.8)
P 		1395.5
(-3)
P 		6177
C 8053.7
(3)
P 		7811.5
(2.8)
P 		6078
(1.2)
P 		1330.7
(-3)
P 		6723
avr 7617.8
(2.8) 		7780.8
(3) 		6797.9
(2.1) 		1405.3
(-3) 		6376

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6751
1 2 3 4 5 6 range
A 9550.7
(-0.4)
P 		9951.2
(-0)
P 		13159
(3)
P 		12546.6
(2.4)
P 		7763
(-2.1)
P 		6796.8
(-3)
P 		6362
B 8328.8
(-0.8)
P 		9199.2
(0.3)
P 		11544.4
(3)
P 		9678.2
(0.8)
P 		8565.1
(-0.5)
P 		6407.7
(-3)
P 		5137
C 9336.5
(-0.2)
P 		9316.5
(-0.3)
P 		11142.2
(2.1)
P 		11883.2
(3)
P 		8206.1
(-1.7)
P 		7175.6
(-3)
P 		4708
avr 9072
(-0.3) 		9489
(0.1) 		11948.5
(3) 		11369.3
(2.3) 		8178.1
(-1.4) 		6793.4
(-3) 		5155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2465
1 2 3 4 range
A 2682.9
(3)
P 		1727.6
(0.7)
P 		1809.1
(0.9)
P 		218.1
(-3)
P 		2465
B 2369.8
(3)
P 		1870.2
(1.6)
P 		1713.7
(1.1)
P 		297.4
(-3)
P 		2072
C 2666
(3)
P 		2245.9
(2)
P 		2118.4
(1.7)
P 		224.4
(-3)
P 		2442
avr 2572.9
(3) 		1947.9
(1.4) 		1880.4
(1.2) 		246.6
(-3) 		2326

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1884
1 2 3 4 5 6 range
A 1760.6
(-2.7)
P 		3539.1
(3)
P 		2865.7
(0.9)
P 		3182.1
(1.9)
P 		2342.5
(-0.8)
P 		1654.8
(-3)
P 		1884
B 2898
(-0.2)
P 		3062
(0.7)
P 		2803.3
(-0.7)
P 		2563.4
(-2)
P 		3479.9
(3)
P 		2389.4
(-3)
P 		1091
C 3460.6
(3)
P 		2842.7
(-0.5)
P 		2835.9
(-0.5)
P 		2389.7
(-3)
P 		2655.4
(-1.5)
P 		2402.1
(-2.9)
P 		1071
avr 2706.4
(0.3) 		3147.9
(3) 		2835
(1.1) 		2711.7
(0.4) 		2825.9
(1.1) 		2148.8
(-3) 		999
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182553_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14428
Sequence Type Exemplar sequence
Representative Public ID C54D10.3 NCBI
Target Description C54D10.3 /REP_DB=WormBase Gene ID /WP=CE05499 /TR=Q18827 /GB=CAA99799.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073715(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073715 NCBI Caenorhabditis elegans C54D10.3 (C54D10.3) mRNA, complete cds. 11/11 None
SNAP00000038018 ENSEMBL cdna:SNAP chromosome:CEL140:V:12421190:12421911:1 11/11 None
GENEFINDER00000038034 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12421190:12421911:1 11/11 None
C54D10.3.1 ENSEMBL cdna:known chromosome:CEL140:V:12421178:12422004:1 gene:C54D10.3 11/11 None
C54D10.3.2 ENSEMBL cdna:known chromosome:CEL140:V:12421180:12422004:1 gene:C54D10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12421189-12421911(+) 94.8 100.0
Public Domain and Genome References
Gene Title
Ensembl C54D10.3
Entrez Gene 179704 Entrez gene
SwissProt Q18827 EMBL-EBI
WormBase CE05499 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99799 Hypothetical protein C54D10.3 [Caenorhabditis elegans] ref|NP_506116.1| C54D10.3 [Caenorhabditis elegans] 2.0E-80
blast CAE75481 Hypothetical protein CBG23485 [Caenorhabditis briggsae] 6.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.8E-14
Sequence
Target Sequence 
>C. ELEGANS:182553_AT
tgctgttcatcattttggtttctacattggtagcttctgcttccgcccaaacatgtgcca
acgaccaaggaccatgtctcaaaatgactgtcaacactatggacatctacatctgtccag
aaggaatggcctgtttgacaaactccacttgttgtgaatatgctgatattcaagtgtcta
ctacgaccaccacaacagctgcatccacctctacttgtgtcgacaaagtcaatccgtcga
caggagtatctgattgcccaaagagtgcttacctgtgcaatgattcaacctattacacat
tgatgactgagcaatgcccaaaaacgtgcaacagatgcagtggtggaaacaataataata
acaacaacaacaacggtggatcatcttcaagctgtgtcgacaaagtgaacccatcaaccg
gagtttctgaatgtgcagctaaagcctacttgtgtaccaactcgatctactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGTTCATCATTTTGGTTTCTAC 231 583 14 Antisense
TCTACATTGGTAGCTTCTGCTTCCG 123 615 34 Antisense
GCCAACGACCAAGGACCATGTCTCA 89 277 70 Antisense
TCAAGTGTCTACTACGACCACCACA 307 633 183 Antisense
CCACCTCTACTTGTGTCGACAAAGT 247 273 218 Antisense
GATTGCCCAAAGAGTGCTTACCTGT 528 433 265 Antisense
GCTTACCTGTGCAATGATTCAACCT 183 305 280 Antisense
GGTGGATCATCTTCAAGCTGTGTCG 495 499 388 Antisense
GCTGTGTCGACAAAGTGAACCCATC 541 293 404 Antisense
GCAGCTAAAGCCTACTTGTGTACCA 378 309 448 Antisense
ACTTGTGTACCAACTCGATCTACTT 257 101 461 Antisense
Sequence Source Affymetrix Proprietary Database