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[182552_at]

Description F22A3.2 /REP_DB=WormBase Gene ID /WP=CE04438 /TR=Q19694 /GB=AAA83193.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 03      4grp_high_8_8 > 46      6grp_highest_nonorm > 53      6grp_total > 61      4grp_total > 41      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 366
1 2 3 4 range
A 609.2
(-3)
P 		944.2
(3)
P 		780.6
(0.1)
P 		747.7
(-0.5)
P 		335
B 587.8
(-3)
P 		891.4
(2.9)
P 		898
(3)
P 		709.3
(-0.6)
P 		310
C 678
(-3)
P 		953.5
(3)
P 		940.4
(2.7)
P 		764.9
(-1.1)
P 		276
avr 625
(-3) 		929.7
(3) 		873
(1.9) 		740.6
(-0.7) 		305

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 812
1 2 3 4 5 6 range
A 696.4
(-1.3)
P 		996.8
(1.1)
P 		1144.1
(2.3)
P 		1235.9
(3)
P 		701.5
(-1.3)
P 		485
(-3)
P 		751
B 641
(-1.4)
P 		876.8
(0.4)
P 		1218.4
(3)
P 		1019.3
(1.5)
P 		775.8
(-0.4)
P 		438.1
(-3)
P 		780
C 729.7
(-0.7)
P 		873.1
(0.4)
P 		952.2
(1)
P 		1220.3
(3)
P 		759.3
(-0.5)
P 		424.2
(-3)
P 		796
avr 689
(-1) 		915.6
(0.9) 		1104.9
(2.5) 		1158.5
(3) 		745.5
(-0.5) 		449.1
(-3) 		709
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 221.8
(3)
P 		205
(2.1)
P 		204.1
(2.1)
P 		109.4
(-3)
P 		112
B 187.5
(0.2)
P 		220.2
(3)
P 		208.1
(1.9)
P 		151.1
(-3)
P 		69
C 224.4
(-0.1)
P 		274.1
(1.4)
P 		327.8
(3)
P 		129
(-3)
P 		199
avr 211.2
(1.2) 		233.1
(2.3) 		246.7
(3) 		129.8
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 128.4
(-2.7)
P 		354.5
(3)
P 		249.2
(0.3)
P 		313.4
(2)
P 		211.7
(-0.6)
P 		118.1
(-3)
P 		236
B 223
(-0.6)
P 		291.9
(2.1)
P 		295.9
(2.2)
P 		270
(1.2)
P 		315.2
(3)
P 		163.4
(-3)
P 		152
C 270.5
(3)
P 		266.4
(2.8)
P 		242.4
(1.7)
P 		245.4
(1.8)
P 		245.7
(1.8)
P 		142
(-3)
P 		129
avr 207.3
(-0.6) 		304.3
(3) 		262.5
(1.5) 		276.3
(2) 		257.5
(1.3) 		141.2
(-3) 		163
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182552_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16212
Sequence Type Exemplar sequence
Representative Public ID F22A3.2 NCBI
Target Description F22A3.2 /REP_DB=WormBase Gene ID /WP=CE04438 /TR=Q19694 /GB=AAA83193.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076697(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076697 NCBI Caenorhabditis elegans F22A3.2 (F22A3.2) mRNA, complete cds. 9/11 None
SNAP00000004709 ENSEMBL cdna:SNAP chromosome:CEL140:X:6512242:6512767:1 9/11 None
GENEFINDER00000004719 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6512242:6512767:1 9/11 None
F22A3.2 ENSEMBL cdna:known chromosome:CEL140:X:6510895:6512850:1 gene:F22A3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004708 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004715 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6510894-6512767(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22A3.2
Entrez Gene 180925 Entrez gene
WormBase CE38505 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE61272 Hypothetical protein CBG05087 [Caenorhabditis briggsae] 2.0E-97
blast NP_509098 F22A3.2 [Caenorhabditis elegans] gb|AAA83193.2| Hypothetical protein F22A3.2 [Caenorhabditis elegans] 1.0E-74
blast NP_509098 F22A3.2 [Caenorhabditis elegans] gb|AAA83193.2| Hypothetical protein F22A3.2 [Caenorhabditis elegans] 6.0E-74
blast AAZ32810 Hypothetical protein F22A3.7 [Caenorhabditis elegans] 4.0E-58
blast CAE61272 Hypothetical protein CBG05087 [Caenorhabditis briggsae] 2.0E-43
blast CAH89266 transthyretin-like protein [Xiphinema index] 2.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001534 EMBL-EBI Transthyretin-like 1.5E-59
Pfam IPR001534 EMBL-EBI Transthyretin-like 7.3E-65
Pfam IPR001534 EMBL-EBI Transthyretin-like 7.3E-65
Sequence
Target Sequence 
>C. ELEGANS:182552_AT
actttctgttgtctgggtcttcaatggagctgacaccaattgatccagaactaagaattt
tccatgactgcaatgatcaaggaagcccttgccaacgagaatgggttatccggataccag
caaaatacattactaatgggccagaagtgaaggaaatcatggatctaggagttttaaatt
tggaggttgagatgatttccaaacttttgatccttggaacatgtgttctatcagttgtct
tggctgcatcggaatcgcactctggacgtcaaaactacagagtgaaaggcgcgtttagat
gtggcaatgttccagtaaaaaatgttcaagtaaagctgatagatgacgactttggatccg
accctgatgatgatttgggtagcggctatacaaacgcaaacggagagttcgaattgagtg
gatccaccacggaactcacaactattgatcctcacctgaaaatctaccacgattgtgatg
acggaattaatccatgccaaagacgttggaagtttgaattgccaaacaactacatttatt
cggacactgacacaccgaagacttttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTCTGTTGTCTGGGTCTTCAAT 318 103 206 Antisense
AGGAAGCCCTTGCCAACGAGAATGG 143 53 285 Antisense
ATGTGTTCTATCAGTTGTCTTGGCT 579 49 426 Antisense
TCTTGGCTGCATCGGAATCGCACTC 125 619 443 Antisense
ATCGCACTCTGGACGTCAAAACTAC 483 37 459 Antisense
TAGATGACGACTTTGGATCCGACCC 239 651 545 Antisense
GGATCCGACCCTGATGATGATTTGG 346 513 559 Antisense
AGTGGATCCACCACGGAACTCACAA 397 59 622 Antisense
GAACTCACAACTATTGATCCTCACC 37 351 637 Antisense
GATCCTCACCTGAAAATCTACCACG 208 417 652 Antisense
GACACTGACACACCGAAGACTTTTG 390 367 748 Antisense
Sequence Source Affymetrix Proprietary Database