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[182532_at]

Description H12D21.5 /REP_DB=WormBase Gene ID /WP=CE20958 /TR=Q9XTW7 /GB=CAB07431.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 61      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 52      6grp_total > 61      4grp_total > 53      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 714.1
(1.8)
P 		799.8
(3)
P 		528.8
(-0.8)
P 		370.4
(-3)
P 		429
B 850.2
(3)
P 		523.2
(-1.2)
P 		598.5
(-0.2)
P 		379.3
(-3)
P 		471
C 788.4
(3)
P 		514.8
(-1)
P 		475.9
(-1.6)
P 		379
(-3)
P 		409
avr 784.2
(3) 		612.6
(0.5) 		534.4
(-0.7) 		376.2
(-3) 		408

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 810
1 2 3 4 5 6 range
A 569.7
(-1.8)
P 		656
(-0.3)
P 		822.7
(2.6)
P 		845.5
(3)
P 		523.2
(-2.6)
P 		500.5
(-3)
P 		345
B 551.7
(-1.2)
P 		458.7
(-2)
P 		984.7
(3)
P 		908.3
(2.3)
P 		602.9
(-0.7)
P 		359.1
(-3)
P 		626
C 571.5
(-1.8)
P 		600.9
(-1.6)
P 		1127.7
(2.7)
P 		1168.9
(3)
P 		745.3
(-0.4)
P 		426.9
(-3)
P 		742
avr 564.3
(-1.5) 		571.9
(-1.4) 		978.4
(3) 		974.2
(3) 		623.8
(-0.9) 		428.8
(-3) 		550
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 217
1 2 3 4 range
A 260
(3)
P 		173.6
(0.5)
P 		138.2
(-0.6)
P 		54.2
(-3)
P 		206
B 271.1
(3)
P 		129.2
(-1.5)
P 		138.7
(-1.2)
P 		80.8
(-3)
P 		190
C 261
(3)
P 		148
(-0.4)
P 		165.9
(0.1)
P 		63.9
(-3)
P 		197
avr 264
(3) 		150.3
(-0.5) 		147.6
(-0.5) 		66.3
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 105
(-3)
P 		233.3
(3)
P 		179.2
(0.5)
P 		214.4
(2.1)
P 		157.9
(-0.5)
P 		121.9
(-2.2)
P 		128
B 192
(0.1)
P 		152.7
(-2)
P 		239.1
(2.7)
P 		240.6
(2.8)
P 		244.9
(3)
P 		133.9
(-3)
P 		111
C 211.8
(-0.1)
P 		183.3
(-1.3)
P 		287
(3)
P 		235.1
(0.8)
P 		241.2
(1.1)
P 		142.9
(-3)
P 		144
avr 169.6
(-0.8) 		189.8
(0.3) 		235.1
(3) 		230
(2.7) 		214.7
(1.8) 		132.9
(-3) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182532_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14236
Sequence Type Exemplar sequence
Representative Public ID H12D21.5 NCBI
Target Description H12D21.5 /REP_DB=WormBase Gene ID /WP=CE20958 /TR=Q9XTW7 /GB=CAB07431.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074273(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074273 NCBI Caenorhabditis elegans H12D21.7 (H12D21.7) mRNA, complete cds. 9/11 None
SNAP00000026964 ENSEMBL cdna:SNAP chromosome:CEL140:V:14898591:14901535:1 9/11 None
GENEFINDER00000026981 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14896674:14899859:1 11/11 None
H12D21.7 ENSEMBL cdna:known chromosome:CEL140:V:14898591:14901601:1 gene:H12D21.7 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_074271 2/11 Cross Hyb Matching Probes None
SNAP00000026963 1/11 Cross Hyb Matching Probes None
H12D21.5 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14896673-14899895(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H12D21.7
Entrez Gene 3565829 Entrez gene
SwissProt Q9XTW9 EMBL-EBI
WormBase CE36178 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590188_AT similar to thiosulfate sulfurtransferase cfa
CANINE:1588038_AT similar to thiosulfate sulfurtransferase cfa
CANINE_2:CFA.14500.1.S1_AT similar to thiosulfate sulfurtransferase cfa
CANINE_2:CFA.14846.1.A1_AT similar to thiosulfate sulfurtransferase cfa
CANINE_2:CFAAFFX.3164.1.S1_S_AT similar to thiosulfate sulfurtransferase cfa
CHICKEN:GGA.5294.1.S1_AT thiosulfate sulfurtransferase (rhodanese) gga
HG-U133_PLUS_2:209605_AT thiosulfate sulfurtransferase (rhodanese) hs
HG-FOCUS:209605_AT thiosulfate sulfurtransferase (rhodanese) hs
HG-U133A:209605_AT thiosulfate sulfurtransferase (rhodanese) hs
HG-U133A_2:209605_AT thiosulfate sulfurtransferase (rhodanese) hs
HUGENEFL:D87292_AT thiosulfate sulfurtransferase (rhodanese) hs
U133_X3P:G1877030_3P_AT thiosulfate sulfurtransferase (rhodanese) hs
HG-U95AV2:36123_AT thiosulfate sulfurtransferase (rhodanese) hs
MU11KSUBA:U35741_S_AT thiosulfate sulfurtransferase, mitochondrial mm
MG-U74AV2:160085_AT thiosulfate sulfurtransferase, mitochondrial mm
MG-U74CV2:171131_R_AT thiosulfate sulfurtransferase, mitochondrial mm
MOE430A:1448609_AT thiosulfate sulfurtransferase, mitochondrial mm
MOUSE430A_2:1448609_AT thiosulfate sulfurtransferase, mitochondrial mm
MOUSE430_2:1448609_AT thiosulfate sulfurtransferase, mitochondrial mm
MU19KSUBB:TC31835_I_AT Thiosulfate sulfurtransferase, mitochondrial, mRNA (cDNA clone MGC:5996 IMAGE:3257034) mm
RG-U34A:X56228_G_AT thiosulfate sulfurtransferase rn
RG-U34A:X56228_AT thiosulfate sulfurtransferase rn
RAE230A:1370881_AT thiosulfate sulfurtransferase rn
RAT230_2:1370881_AT thiosulfate sulfurtransferase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
8272 sulfate transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4792 thiosulfate sulfurtransferase activity inferred from electronic annotation QuickGO AmiGO
5488 binding inferred from electronic annotation QuickGO AmiGO
8289 lipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07429 Hypothetical protein H12D21.7 [Caenorhabditis elegans] ref|NP_506674.2| H12D21.7 [Caenorhabditis elegans] 1.0E-168
blast CAB07425 Hypothetical protein H12D21.4 [Caenorhabditis elegans] ref|NP_506671.1| H12D21.4 [Caenorhabditis elegans] 1.0E-112
blast CAB07429 Hypothetical protein H12D21.7 [Caenorhabditis elegans] ref|NP_506674.2| H12D21.7 [Caenorhabditis elegans] 1.0E-70
blast CAE60835 Hypothetical protein CBG04544 [Caenorhabditis briggsae] 5.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR001763 EMBL-EBI Rhodanese-like 3.7E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026981 2 12-31,76-98
Sequence
Target Sequence 
>C. ELEGANS:182532_AT
gagattaccgcgtttatcggtccgcaaatgataatgaaacatcttcttctggtccttgca
ccgatacagtaatttacaaaccagtggaatggccatggtacatgattgcagccattgctg
gactatcaattttaaatgttcttctcataattgaaattattcgaattggcaggaaatggc
tcaaactgaggaaagaagctatgagtacaaaccctattgtcactccacaatggttgatgc
aaaatgtcaataaagtacgattaattgatgctacgtatgcaccaacagccactcgtgact
acaaggagttcaagaccaaatgttacggaaattttgatttattgacaaaaaataatactg
tggctgcgtataccggagagcatattccaggagctgcacactttggccttgactctgcct
atttcccatcgcagtacataaaatttgatctgtacccgccagagcattttgaaaaatatt
tacgcttgttgggaatcaacaatgacgaacaggtggtcatctacagcagaggtcctgccg
caggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATTACCGCGTTTATCGGTCCGC 606 395 146 Antisense
TCTGGTCCTTGCACCGATACAGTAA 143 609 193 Antisense
AGTACAAACCCTATTGTCACTCCAC 179 63 349 Antisense
GATGCTACGTATGCACCAACAGCCA 200 413 412 Antisense
CCAACAGCCACTCGTGACTACAAGG 72 277 427 Antisense
TACTGTGGCTGCGTATACCGGAGAG 96 647 501 Antisense
GCATATTCCAGGAGCTGCACACTTT 255 309 525 Antisense
CCTATTTCCCATCGCAGTACATAAA 405 269 563 Antisense
AAATTTGATCTGTACCCGCCAGAGC 111 117 586 Antisense
GAACAGGTGGTCATCTACAGCAGAG 351 351 652 Antisense
TACAGCAGAGGTCCTGCCGCAGGAA 281 641 667 Antisense
Sequence Source Affymetrix Proprietary Database