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[182527_at]

Description T19B4.5 /REP_DB=WormBase Gene ID /WP=CE13744 /TR=P91453 /GB=AAB37631.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 1.3
(-3)
A 		1.8
(-2.5)
A 		2.1
(-2.2)
A 		7.6
(3)
A 		6
B 1.3
(-3)
A 		1.6
(-2.4)
A 		1.3
(-3)
A 		4.5
(3)
A 		3
C 2.2
(-3)
A 		2.3
(-3)
A 		2.4
(-3)
A 		40.6
(3)
A 		38
avr 1.6
(-3) 		1.9
(-2.9) 		1.9
(-2.9) 		17.6
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 1.8
(-2.7)
A 		1.2
(-3)
A 		1.4
(-2.9)
A 		1.6
(-2.8)
A 		2.2
(-2.6)
A 		15.5
(3)
A 		14
B 1.3
(-3)
A 		1.7
(-2.9)
A 		1.5
(-3)
A 		2.4
(-2.7)
A 		1.8
(-2.9)
A 		26.5
(3)
A 		25
C 1.5
(-2.9)
A 		2.8
(-2.8)
A 		1.7
(-2.9)
A 		10.2
(-1.7)
A 		1.1
(-3)
A 		42.6
(3)
A 		42
avr 1.5
(-3) 		1.9
(-2.9) 		1.5
(-3) 		4.7
(-2.3) 		1.7
(-3) 		28.2
(3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.5
(-2.1)
A 		0.4
(-3)
A 		0.5
(-2.1)
A 		1.1
(3)
A 		1
B 0.4
(-2.1)
A 		0.4
(-2.1)
A 		0.3
(-3)
A 		1
(3)
A 		1
C 0.7
(-3)
A 		0.7
(-3)
A 		0.8
(-2.9)
A 		6.8
(3)
A 		6
avr 0.5
(-3) 		0.5
(-3) 		0.5
(-3) 		3
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.3
(-3)
A 		0.4
(-2.8)
A 		0.3
(-3)
A 		0.4
(-2.8)
A 		0.7
(-2.3)
A 		3.8
(3)
A 		4
B 0.5
(-2.9)
A 		0.6
(-2.9)
A 		0.4
(-3)
A 		0.6
(-2.9)
A 		0.7
(-2.8)
A 		9.9
(3)
A 		10
C 0.5
(-3)
A 		0.9
(-2.8)
A 		0.4
(-3)
A 		2
(-2.3)
A 		0.4
(-3)
A 		14.3
(3)
A 		14
avr 0.4
(-3) 		0.6
(-2.9) 		0.4
(-3) 		1
(-2.6) 		0.6
(-2.9) 		9.3
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.991
Sequence Type Exemplar sequence
Representative Public ID T19B4.5 NCBI
Target Description T19B4.5 /REP_DB=WormBase Gene ID /WP=CE13744 /TR=P91453 /GB=AAB37631.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059260(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059260 NCBI Caenorhabditis elegans T19B4.5 (T19B4.5) mRNA, complete cds. 11/11 None
SNAP00000019534 ENSEMBL cdna:SNAP chromosome:CEL140:I:5674322:5676241:1 11/11 None
GENEFINDER00000019544 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5674322:5676241:1 11/11 None
T19B4.5 ENSEMBL cdna:known chromosome:CEL140:I:5674412:5676241:1 gene:T19B4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5674411-5676241(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T19B4.5
Entrez Gene 188582 Entrez gene
SwissProt P91453 EMBL-EBI
WormBase CE13744 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37631 Hypothetical protein T19B4.5 [Caenorhabditis elegans] ref|NP_491661.1| T19B4.5 [Caenorhabditis elegans] 0.0
blast CAE68934 Hypothetical protein CBG14914 [Caenorhabditis briggsae] 1.0E-148
blast CAE68934 Hypothetical protein CBG14914 [Caenorhabditis briggsae] 1.0E-140
Sequence
Target Sequence 
>C. ELEGANS:182527_AT
gacagcatccgaacatgctcttatcgaaatcgagaataatatcaaacaaacaatagaaga
tgcaaaagtcgatactgagagattaagagaattacaagaagctgacttctcacaggatga
atatcatcaatatcttggaatgttgattatggatctggaaagatcaaatgaggcgaactc
gactctgacaacatattatcgcgaacgacaacgacatgaaaagactatttcggaagccag
agaaaatgcattttcagctagaattcgtcagttggagactgaaaatcgaaaattgaggga
ttcgattcatgtgatgtattatggaaagttcaatgaggctggaggtcataacaatattta
tcgaaatgatcatatgggtcaaatgatggttgaagagcccgaagagcatgttgttattga
aggacacgtgggcaataatcaagaaggagaagaagttccacaagagtggattgtcgagga
gactgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGCATCCGAACATGCTCTTATC 229 369 594 Antisense
GAAGCTGACTTCTCACAGGATGAAT 575 343 691 Antisense
ATGAGGCGAACTCGACTCTGACAAC 515 45 761 Antisense
AACTCGACTCTGACAACATATTATC 698 141 769 Antisense
TTATCGCGAACGACAACGACATGAA 668 677 789 Antisense
TTTCAGCTAGAATTCGTCAGTTGGA 526 669 845 Antisense
TTGAGGGATTCGATTCATGTGATGT 656 701 886 Antisense
GAGGCTGGAGGTCATAACAATATTT 246 405 928 Antisense
GAGCCCGAAGAGCATGTTGTTATTG 345 385 988 Antisense
GAAGGACACGTGGGCAATAATCAAG 676 331 1012 Antisense
GAGTGGATTGTCGAGGAGACTGTTG 680 401 1057 Antisense
Sequence Source Affymetrix Proprietary Database