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[182498_at]

Description H25K10.1 /REP_DB=WormBase Gene ID /WP=CE19933 /TR=Q9U3D3 /GB=CAB63230.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 52      4grp_total > 56      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 3
(-3)
A 		50.8
(-0.6)
A 		121.9
(3)
A 		6.9
(-2.8)
A 		119
B 5.7
(-2.9)
A 		104.9
(3)
A 		102.5
(2.9)
A 		3.5
(-3)
A 		101
C 2
(-3)
A 		75.3
(0.6)
A 		125.7
(3)
P 		7.5
(-2.7)
A 		124
avr 3.6
(-3) 		77
(0.9) 		116.7
(3) 		6
(-2.9) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 597
1 2 3 4 5 6 range
A 16.6
(-2.9)
A 		117.9
(-1.9)
A 		599.9
(3)
P 		207.3
(-1)
P 		121.2
(-1.8)
A 		6.4
(-3)
A 		594
B 5.4
(-3)
A 		159.2
(-1.3)
P 		548
(3)
P 		175.7
(-1.1)
P 		98.4
(-2)
A 		26.1
(-2.8)
A 		543
C 3
(-3)
A 		160.8
(-1.1)
P 		499.1
(3)
P 		212.6
(-0.5)
P 		116.7
(-1.6)
P 		6
(-3)
A 		496
avr 8.3
(-3) 		146
(-1.5) 		549
(3) 		198.5
(-0.9) 		112.1
(-1.8) 		12.8
(-3) 		541
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 1.1
(-3)
A 		11
(-1.1)
A 		31.9
(3)
A 		1
(-3)
A 		31
B 1.8
(-2.7)
A 		25.9
(3)
A 		23.8
(2.5)
A 		0.7
(-3)
A 		25
C 0.7
(-3)
A 		21.7
(-0.1)
A 		43.8
(3)
P 		1.3
(-2.9)
A 		43
avr 1.2
(-3) 		19.5
(0.4) 		33.2
(3) 		1
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 3.1
(-2.9)
A 		41.9
(-1.1)
A 		130.7
(3)
P 		52.6
(-0.6)
P 		36.6
(-1.4)
A 		1.5
(-3)
A 		129
B 1.9
(-3)
A 		53
(-0.7)
P 		133.1
(3)
P 		46.5
(-1)
P 		40
(-1.3)
A 		9.7
(-2.6)
A 		131
C 1.1
(-3)
A 		49.1
(-0.7)
P 		127
(3)
P 		42.8
(-1)
P 		37.7
(-1.3)
P 		2
(-3)
A 		126
avr 2
(-3) 		48
(-0.8) 		130.3
(3) 		47.3
(-0.9) 		38.1
(-1.3) 		4.4
(-2.9) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182498_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10042
Sequence Type Exemplar sequence
Representative Public ID H25K10.1 NCBI
Target Description H25K10.1 /REP_DB=WormBase Gene ID /WP=CE19933 /TR=Q9U3D3 /GB=CAB63230.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070519(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070519 NCBI Caenorhabditis elegans H25K10.1 (H25K10.1) mRNA, complete cds. 11/11 None
SNAP00000037532 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16204040:16205225:1 10/11 None
GENEFINDER00000037548 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16204040:16206697:1 11/11 None
H25K10.1 ENSEMBL cdna:known chromosome:CEL140:IV:16204040:16205667:1 gene:H25K10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16204045-16205673(+) 99.53 100.0
Public Domain and Genome References
Gene Title
Ensembl H25K10.1
Entrez Gene 186777 Entrez gene
SwissProt Q9U3D3 EMBL-EBI
WormBase CE19933 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63230 Hypothetical protein H25K10.1 [Caenorhabditis elegans] ref|NP_502920.1| H25K10.1 [Caenorhabditis elegans] 0.0
blast CAB54350 Hypothetical protein Y105C5B.3 [Caenorhabditis elegans] ref|NP_502892.2| Y105C5B.3 [Caenorhabditis elegans] 1.0E-140
blast CAB54350 Hypothetical protein Y105C5B.3 [Caenorhabditis elegans] ref|NP_502892.2| Y105C5B.3 [Caenorhabditis elegans] 1.0E-139
blast CAE57394 Hypothetical protein CBG00344 [Caenorhabditis briggsae] 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 7.0E-21
Sequence
Target Sequence 
>C. ELEGANS:182498_AT
gggtgattgtaatgctccatagaccatggtactgctcaaatgagacaccggaaggatgta
atgatggttgggatacccttccaagacaaggactcgggaagctatttcctggattagagg
atttattgaatgagtatacagttgatatggtattatatggacacaggcatacttacgaga
gaatgtggccaatttataataagaatccatataaatcagagaatcctggacatatcaaaa
atgcacctgcaccagtttatattttgactggatcagcgggatgccactcccatgaagacc
catctgatcacataatgcaggatttttctgtgaaagctctcggagaatacgggtacactt
accttaccgtacataattcaacacatttgttcacggattttgtggatacattcagtggaa
atataacttcattagactctttcgttttggagaagaactcaacgcttacttgtattttcc
tgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTGATTGTAATGCTCCATAGACC 16 497 482 Antisense
GACCATGGTACTGCTCAAATGAGAC 699 383 503 Antisense
GATGGTTGGGATACCCTTCCAAGAC 125 407 544 Antisense
GGACTCGGGAAGCTATTTCCTGGAT 631 521 571 Antisense
AAAAATGCACCTGCACCAGTTTATA 456 129 718 Antisense
TGACTGGATCAGCGGGATGCCACTC 685 569 746 Antisense
AGCTCTCGGAGAATACGGGTACACT 482 63 816 Antisense
TACACTTACCTTACCGTACATAATT 596 637 835 Antisense
CAACACATTTGTTCACGGATTTTGT 670 187 860 Antisense
AACTTCATTAGACTCTTTCGTTTTG 254 139 906 Antisense
CAACGCTTACTTGTATTTTCCTGTT 523 187 941 Antisense
Sequence Source Affymetrix Proprietary Database