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[182497_at]

Description F28B3.1 /REP_DB=WormBase Gene ID /WP=CE09739 /TR=O01787 /GB=AAK21373.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 27      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 70      6grp_total > 27      4grp_total > 54      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 509.2
(3)
P 		393.3
(-0.1)
P 		361.5
(-1)
P 		285.6
(-3)
P 		224
B 523.4
(3)
P 		327.3
(-2.1)
P 		291.1
(-3)
P 		328.4
(-2)
P 		232
C 497.7
(3)
P 		467.5
(2.3)
P 		348.5
(-0.5)
P 		240.4
(-3)
P 		257
avr 510.1
(3) 		396
(-0) 		333.7
(-1.7) 		284.8
(-3) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 576
1 2 3 4 5 6 range
A 395.8
(1.7)
P 		471
(3)
P 		199.8
(-1.9)
P 		135.9
(-3)
P 		198.7
(-1.9)
P 		403.1
(1.8)
P 		335
B 317.5
(3)
P 		271.6
(0.4)
P 		263.5
(-0)
P 		229.3
(-1.9)
P 		210.1
(-3)
P 		264.8
(0.1)
P 		107
C 712.3
(3)
P 		274.7
(-1.7)
P 		342.1
(-0.9)
P 		149.7
(-3)
P 		292
(-1.5)
P 		261.7
(-1.8)
P 		563
avr 475.2
(3) 		339.1
(0.3) 		268.5
(-1.1) 		171.6
(-3) 		233.6
(-1.8) 		309.9
(-0.3) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 185.4
(3)
P 		85.4
(-1.2)
P 		94.5
(-0.8)
P 		41.8
(-3)
P 		144
B 166.9
(3)
P 		80.8
(-2.2)
P 		67.5
(-3)
P 		70
(-2.8)
P 		99
C 164.7
(3)
P 		134.4
(1.5)
P 		121.5
(0.9)
P 		40.5
(-3)
P 		124
avr 172.3
(3) 		100.2
(-0.6) 		94.5
(-0.8) 		50.8
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 73
(-1.3)
P 		167.5
(3)
P 		43.5
(-2.6)
P 		34.5
(-3)
P 		59.9
(-1.9)
P 		98.1
(-0.1)
P 		133
B 110.5
(3)
P 		90.4
(0.6)
P 		64
(-2.6)
P 		60.7
(-3)
P 		85.4
(-0)
P 		98.7
(1.6)
P 		50
C 264
(3)
P 		83.8
(-1.6)
P 		87.1
(-1.5)
P 		30.1
(-3)
P 		94.5
(-1.3)
P 		87.6
(-1.5)
P 		234
avr 149.2
(3) 		113.9
(1) 		64.9
(-1.7) 		41.8
(-3) 		79.9
(-0.9) 		94.8
(-0) 		107
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182497_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1060
Sequence Type Exemplar sequence
Representative Public ID F28B3.1 NCBI
Target Description F28B3.1 /REP_DB=WormBase Gene ID /WP=CE09739 /TR=O01787 /GB=AAK21373.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059082(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059082 NCBI Caenorhabditis elegans F28B3.1 (F28B3.1) mRNA, complete cds. 11/11 None
GENEFINDER00000020914 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4937771:4948942:1 11/11 None
SNAP00000020907 ENSEMBL cdna:SNAP chromosome:CEL140:I:4943691:4948942:1 11/11 None
F28B3.1 ENSEMBL cdna:known chromosome:CEL140:I:4943024:4949329:1 gene:F28B3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4943023-4948942(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28B3.1
Entrez Gene 172113 Entrez gene
SwissProt O01787 EMBL-EBI
WormBase CE36644 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9413.1.S1_S_AT similar to Protein KIAA0934 cfa
CANINE_2:CFAAFFX.9422.1.S1_S_AT similar to Protein KIAA0934 cfa
DROSOPHILA_2:1634461_AT DISCO Interacting Protein 2 dm
DROSGENOME1:152595_AT DISCO Interacting Protein 2 dm
CHICKEN:GGAAFFX.4144.1.S1_S_AT similar to Hypothetical protein KIAA0934 gga
CHICKEN:GGAAFFX.4144.3.S1_S_AT similar to Hypothetical protein KIAA0934 gga
HG-U95AV2:33407_AT KIAA0934 hs
HU35KSUBA:RC_AA453790_AT KIAA0934 hs
HU35KSUBC:RC_N27845_AT KIAA0934 hs
HU35KSUBD:RC_AA410299_AT KIAA0934 hs
U133_X3P:HS2.411370.1.S1_3P_S_AT KIAA0934 hs
U133_X3P:HS.227716.0.S2_3P_A_AT KIAA0934 hs
U133_X3P:HS.227716.0.S1_3P_AT KIAA0934 hs
HG-U133_PLUS_2:1565681_S_AT KIAA0934 hs
HG-U133A_2:212503_S_AT KIAA0934 hs
HG-U133_PLUS_2:212503_S_AT KIAA0934 hs
HG-U133A:212503_S_AT KIAA0934 hs
HG-U133A_2:212504_AT KIAA0934 hs
HG-U133_PLUS_2:212504_AT KIAA0934 hs
HG-U133A:212504_AT KIAA0934 hs
HG-U95AV2:33408_AT KIAA0934 hs
U133_X3P:HS2.397085.1.S1_3P_AT KIAA0934 hs
HG-U133_PLUS_2:1560763_AT KIAA0934 hs
HG-U95C:52310_AT KIAA0934 hs
HG-U133_PLUS_2:1565680_AT KIAA0934 hs
HG-U133_PLUS_2:238354_X_AT KIAA0934 hs
HG-U133B:238354_X_AT KIAA0934 hs
HG-U133_PLUS_2:238358_X_AT KIAA0934 hs
HG-U133B:238358_X_AT KIAA0934 hs
HG-U95C:63763_AT KIAA0934 hs
HG-U95D:76512_AT KIAA0934 hs
HG-U95E:89094_AT KIAA0934 hs
HG-U95D:90172_S_AT KIAA0934 hs
HG-U95D:90174_R_AT KIAA0934 hs
HU35KSUBC:RC_H85034_AT KIAA0934 hs
U133_X3P:HS.145492.0.A1_3P_AT KIAA0934 hs
U133_X3P:HS.145492.0.A1_3P_X_AT KIAA0934 hs
U133_X3P:HS.145492.0.S1_3P_AT KIAA0934 hs
MG-U74BV2:107787_AT RIKEN cDNA 2900024P20 gene mm
MOE430B:1429064_AT RIKEN cDNA 2900024P20 gene mm
MOUSE430_2:1429064_AT RIKEN cDNA 2900024P20 gene mm
MOE430B:1456122_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MOUSE430_2:1456122_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MG-U74CV2:136439_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MOUSE430_2:1457180_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MOE430B:1457180_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MOUSE430_2:1458309_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
MOE430B:1458309_AT RIKEN cDNA 2900024P20 gene, mRNA (cDNA clone IMAGE:30545711) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4869 cysteine protease inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21373 Hypothetical protein F28B3.1 [Caenorhabditis elegans] ref|NP_491483.2| F28B3.1 [Caenorhabditis elegans] 0.0
blast CAE73119 Hypothetical protein CBG20501 [Caenorhabditis briggsae] 0.0
blast XP_391894 PREDICTED: similar to ENSANGP00000025395 [Apis mellifera] 0.0
Protein Families
Method ID Description E-Value
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 8.0E-11
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 3.0E-57
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 1.0E-10
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 1.0E-58
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 8.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.1E-13
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.9E-6
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.7E-12
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.5E-4
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.5E-9
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.9E-6
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.7E-12
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.5E-4
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.1E-13
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.9E-6
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.7E-12
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.5E-4
Sequence
Target Sequence 
>C. ELEGANS:182497_AT
caatgcgcagattctcatctcggagaaatttgggtggcgagtattcacaacgcatcccca
ttaaatcgtttggcaagtgtcactgggttcggagacgaaggaacgatgaatacggatgtc
tataatgctcgtttgacgactggagacacaaagactcgatgggctcggacgggatacctg
ggtttccttcgacaaactcaatcgatcaccgagcatggagagcttcatgatgcagttttt
gttgttggagctctcaatgagagtttggttcttcgtggtatgagatatcatccatttgat
gtggagagtactgttagcaaagcgcatcgttttgttggaaattcagccgtcttcacatgg
aatcaccttgtcgttattgccgccgaatgtactggctccgagtcggatgctctcgatctc
gtaccagccatcacgtcagctgtccttgaggaacatcatctcatcgtcggagtcgttgtc
gtcgtggatcctggatctatccgacatgggccaggcggtgagaagctgcggagcactatt
cgctccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGCGCAGATTCTCATCTCGGAG 708 181 3601 Antisense
TGGCGAGTATTCACAACGCATCCCC 391 545 3635 Antisense
ACGCATCCCCATTAAATCGTTTGGC 316 91 3650 Antisense
TGGAAATTCAGCCGTCTTCACATGG 697 547 3936 Antisense
ATGGAATCACCTTGTCGTTATTGCC 290 53 3957 Antisense
TATTGCCGCCGAATGTACTGGCTCC 151 663 3975 Antisense
CGAGTCGGATGCTCTCGATCTCGTA 338 249 3999 Antisense
TCACGTCAGCTGTCCTTGAGGAACA 702 625 4031 Antisense
GGAACATCATCTCATCGTCGGAGTC 357 529 4050 Antisense
ATCTATCCGACATGGGCCAGGCGGT 655 29 4095 Antisense
AAGCTGCGGAGCACTATTCGCTCCC 322 149 4123 Antisense
Sequence Source Affymetrix Proprietary Database