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[182491_at]

Description F53F4.13 /REP_DB=WormBase Gene ID /WP=CE10978 /TR=Q20724 /GB=CAB01208.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 07      4grp_high_8_8 > 20      6grp_highest_nonorm > 07      6grp_total > 40      4grp_total > 27      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5087
1 2 3 4 range
A 9497.4
(-2)
P 		11221.1
(0)
P 		8671.5
(-3)
P 		13758.7
(3)
P 		5087
B 11153.9
(1)
P 		10405.7
(-3)
P 		10751.2
(-1.2)
P 		11530.1
(3)
P 		1124
C 10074.5
(-0.3)
P 		11485.7
(3)
P 		8955
(-3)
P 		11480.5
(3)
P 		2531
avr 10241.9
(-1.3) 		11037.5
(0.4) 		9459.2
(-3) 		12256.4
(3) 		2797

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8278
1 2 3 4 5 6 range
A 14069.5
(1.3)
P 		13926.6
(1.2)
P 		16412.6
(3)
P 		14122
(1.3)
P 		8620.4
(-2.6)
P 		8135.1
(-3)
P 		8278
B 13598.8
(0.6)
P 		15197.4
(2)
P 		16387.5
(3)
P 		12018.8
(-0.8)
P 		9968.7
(-2.5)
P 		9414.4
(-3)
P 		6973
C 13401.7
(0.8)
P 		15669.9
(3)
P 		12107.8
(-0.5)
P 		14465.2
(1.8)
P 		9534
(-3)
P 		10643.9
(-1.9)
P 		6136
avr 13690
(1.6) 		14931.3
(3) 		14969.3
(3) 		13535.3
(1.5) 		9374.4
(-3) 		9397.8
(-3) 		5595
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1621
1 2 3 4 range
A 3458
(3)
P 		2435.8
(-1.2)
P 		2266.9
(-1.9)
P 		2014
(-3)
P 		1444
B 3556.9
(3)
P 		2570
(-2.4)
P 		2491.3
(-2.8)
P 		2457.1
(-3)
P 		1100
C 3335
(3)
P 		3302.2
(2.9)
P 		3121.1
(2.1)
P 		1935.8
(-3)
P 		1399
avr 3450
(3) 		2769.3
(-0.1) 		2626.4
(-0.8) 		2135.6
(-3) 		1314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3078
1 2 3 4 5 6 range
A 2593.6
(-1.8)
P 		4953
(3)
P 		3574.3
(0.2)
P 		3581.7
(0.2)
P 		2601.3
(-1.7)
P 		1980.6
(-3)
P 		2972
B 4731.7
(2)
P 		5058.6
(3)
P 		3979.4
(-0.5)
P 		3183.4
(-3)
P 		4050.2
(-0.2)
P 		3510.6
(-2)
P 		1875
C 4967.4
(3)
P 		4781.4
(2.5)
P 		3081.6
(-2.5)
P 		2909
(-3)
P 		3085.1
(-2.5)
P 		3563.1
(-1.1)
P 		2058
avr 4097.6
(0.4) 		4931
(3) 		3545.1
(-1.3) 		3224.7
(-2.4) 		3245.5
(-2.3) 		3018.1
(-3) 		1913
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182491_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13971
Sequence Type Exemplar sequence
Representative Public ID F53F4.13 NCBI
Target Description F53F4.13 /REP_DB=WormBase Gene ID /WP=CE10978 /TR=Q20724 /GB=CAB01208.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073970(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073970 NCBI Caenorhabditis elegans F53F4.13 (F53F4.13) mRNA, complete cds. 11/11 None
SNAP00000012439 ENSEMBL cdna:SNAP chromosome:CEL140:V:13614009:13614456:-1 11/11 None
GENEFINDER00000012454 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13614009:13620315:-1 9/11 None
F53F4.13 ENSEMBL cdna:known chromosome:CEL140:V:13613928:13614480:-1 gene:F53F4.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13614008-13614456(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F4.13
Entrez Gene 179846 Entrez gene
SwissProt Q20724 EMBL-EBI
WormBase CE10978 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01206 Hypothetical protein F53F4.8 [Caenorhabditis elegans] ref|NP_506373.1| F53F4.8 [Caenorhabditis elegans] 0.0
blast CAE66278 Hypothetical protein CBG11523 [Caenorhabditis briggsae] 0.0
blast CAE59001 Hypothetical protein CBG02276 [Caenorhabditis briggsae] 0.0
blast CAB01208 Hypothetical protein F53F4.13 [Caenorhabditis elegans] ref|NP_506371.1| F53F4.13 [Caenorhabditis elegans] 2.0E-47
blast CAE66280 Hypothetical protein CBG11525 [Caenorhabditis briggsae] 3.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR000519 EMBL-EBI P-type trefoil domain 1.4E-6
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506371.1 1 4-21
F53F4.13 1 4-21
SNAP00000012439 1 4-21
GENEFINDER00000012454 2 1133-1155,1176-1195
Sequence
Target Sequence 
>C. ELEGANS:182491_AT
gaactctatctggacatctctttttgtagcaattctcgctgtttccgttattgccctcaa
cgagaatgaaaacaattatctgcaagagttgctcgatgctggaatttcccaggaaaccgc
taacaagcttgtagacatcacagccagccacaacaacgatggagaaatttctgagaaatc
aggaaaaactattttccaagaaatcatttctgagactgatgcagctatcaaacaagcacc
agctaatgatcagcaagcctacaaggccttcgttgaaagcaaggcagctgaattcggtca
acc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTCTATCTGGACATCTCTTTTT 507 349 15 Antisense
TTGTAGCAATTCTCGCTGTTTCCGT 597 707 38 Antisense
TTTCCGTTATTGCCCTCAACGAGAA 46 671 56 Antisense
ATCTGCAAGAGTTGCTCGATGCTGG 35 35 92 Antisense
TGCTCGATGCTGGAATTTCCCAGGA 632 581 104 Antisense
TTTCCCAGGAAACCGCTAACAAGCT 705 663 119 Antisense
GCTTGTAGACATCACAGCCAGCCAC 423 305 141 Antisense
GATGCAGCTATCAAACAAGCACCAG 395 413 232 Antisense
GCACCAGCTAATGATCAGCAAGCCT 275 315 250 Antisense
AGCCTACAAGGCCTTCGTTGAAAGC 148 75 270 Antisense
GCAAGGCAGCTGAATTCGGTCAACC 137 321 293 Antisense
Sequence Source Affymetrix Proprietary Database