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[182489_at]

Description F53B3.2 /REP_DB=WormBase Gene ID /WP=CE04650 /TR=Q20710 /GB=AAA81405.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 13      4grp_total > 57      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 7.9
(-3)
A 		212.8
(3)
A 		148.6
(1.1)
A 		17.2
(-2.7)
A 		205
B 19.2
(-3)
A 		194.5
(3)
A 		171.6
(2.2)
A 		32.8
(-2.5)
A 		175
C 19.1
(-3)
A 		126.9
(3)
A 		127.8
(3)
A 		26.4
(-2.6)
A 		109
avr 15.4
(-3) 		178.1
(3) 		149.3
(1.9) 		25.5
(-2.6) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 113.8
(3)
A 		51.7
(-1.4)
A 		93.2
(1.6)
A 		96.1
(1.8)
A 		53
(-1.3)
A 		28.5
(-3)
A 		85
B 65.9
(-1.4)
A 		99.9
(3)
A 		58.2
(-2.4)
A 		80.8
(0.5)
A 		72.2
(-0.6)
A 		53.8
(-3)
A 		46
C 47
(-2.9)
A 		63.8
(-0.9)
A 		67.6
(-0.5)
A 		46.1
(-3)
A 		69.5
(-0.2)
A 		96.9
(3)
A 		51
avr 75.6
(3) 		71.8
(1.6) 		73
(2) 		74.3
(2.5) 		64.9
(-1) 		59.7
(-3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 2.9
(-2.9)
A 		46.2
(3)
A 		38.9
(2)
A 		2.5
(-3)
A 		44
B 6.1
(-3)
A 		48
(3)
A 		39.8
(1.8)
A 		7
(-2.9)
A 		42
C 6.3
(-2.7)
A 		36.5
(1.8)
A 		44.5
(3)
A 		4.4
(-3)
A 		40
avr 5.1
(-2.9) 		43.6
(3) 		41.1
(2.6) 		4.6
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 21
(1.8)
A 		18.4
(0.9)
A 		20.3
(1.6)
A 		24.4
(3)
A 		16
(0.1)
A 		6.9
(-3)
A 		18
B 22.9
(-0.2)
A 		33.2
(3)
A 		14.1
(-3)
A 		21.4
(-0.7)
A 		29.3
(1.8)
A 		20.1
(-1.1)
A 		19
C 17.4
(-0.9)
A 		19.5
(-0.4)
A 		17.2
(-0.9)
A 		9.3
(-3)
A 		22.5
(0.4)
A 		32.4
(3)
A 		23
avr 20.4
(-0) 		23.7
(3) 		17.2
(-3) 		18.4
(-1.9) 		22.6
(2) 		19.8
(-0.6) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182489_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18243
Sequence Type Exemplar sequence
Representative Public ID F53B3.2 NCBI
Target Description F53B3.2 /REP_DB=WormBase Gene ID /WP=CE04650 /TR=Q20710 /GB=AAA81405.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076094(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076094 NCBI Caenorhabditis elegans F53B3.2 (F53B3.2) mRNA, complete cds. 11/11 None
GENEFINDER00000011076 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2858089:2862424:-1 11/11 None
SNAP00000011070 ENSEMBL cdna:SNAP chromosome:CEL140:X:2858159:2862436:-1 11/11 None
F53B3.2.2 ENSEMBL cdna:known chromosome:CEL140:X:2857872:2862444:-1 gene:F53B3.2 11/11 None
F53B3.2.1 ENSEMBL cdna:known chromosome:CEL140:X:2858028:2862465:-1 gene:F53B3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2858158-2862424(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53B3.2
Entrez Gene 186152 Entrez gene
SwissProt Q20710 EMBL-EBI
WormBase CE35721 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8171 O-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81405 Hypothetical protein F53B3.2 [Caenorhabditis elegans] ref|NP_508495.2| F53B3.2 [Caenorhabditis elegans] 0.0
blast CAE58823 Hypothetical protein CBG02033 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 3.1E-8
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 1.3E-5
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 3.1E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508495.2 1 7-26
F53B3.2.2 1 7-26
F53B3.2.1 1 7-26
GENEFINDER00000011076 1 7-26
SNAP00000011070 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:182489_AT
agatttgctcagggacacccaaaccgcttcatcactcccacaacacatttcttggcacag
ttcagtgaccccggaaattgtgcaactagttcaaactttaatgtcactttatagaccttc
aagatgtttagtaattggagtattcactggtcttggattactcggtgtcgccgaaagagt
tgattctcgaggaattgttgtcgcacttgagcatccggcctatgctgttttctgggataa
aattgggcagaagcacgcaagaaaactcacggctacgcaaatgtccagaattcaaatgag
aacatctgaaagcatcgaaaaatcattgcctcgacttgcaacaaatgaacccaacacttt
cgattttgtgtttctggatgattttaagagggaaaactatttggatgattatgaacacgc
aattcgactgattcgaagtggtggtcttttaatcattaaccaggcactcaatggtggtgg
agtgatgtcatcgattgatcacatgaccaacgatgacagggtcattcgaaacatgaacat
acgcatcaaacaagacccccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATTTGCTCAGGGACACCCAAACC 567 65 1350 Antisense
TCACTCCCACAACACATTTCTTGGC 680 619 1381 Antisense
TTCTTGGCACAGTTCAGTGACCCCG 653 689 1398 Antisense
TGGTCTTGGATTACTCGGTGTCGCC 651 551 1497 Antisense
AATTGTTGTCGCACTTGAGCATCCG 368 179 1542 Antisense
GGCCTATGCTGTTTTCTGGGATAAA 11 545 1566 Antisense
GCACGCAAGAAAACTCACGGCTACG 278 315 1602 Antisense
CTCACGGCTACGCAAATGTCCAGAA 616 225 1615 Antisense
AATCATTGCCTCGACTTGCAACAAA 292 171 1670 Antisense
ATGAACCCAACACTTTCGATTTTGT 636 25 1694 Antisense
ACATACGCATCAAACAAGACCCCCG 582 103 1886 Antisense
Sequence Source Affymetrix Proprietary Database