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[182487_s_at]

Description EGAP2.3 /REP_DB=WormBase Gene ID /WP=CE04325 /TR=Q19076 /GB=AAA83593.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 12      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 21      6grp_total > 24      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1355
1 2 3 4 range
A 1968.3
(3)
P 		1501.2
(0.8)
P 		1487.3
(0.7)
P 		688
(-3)
P 		1280
B 1927.9
(3)
P 		1637
(1.7)
P 		1697.5
(1.9)
P 		622.5
(-3)
P 		1305
C 1977.3
(3)
P 		1161.6
(-0.6)
P 		1845
(2.4)
P 		622.9
(-3)
P 		1354
avr 1957.8
(3) 		1433.3
(0.6) 		1676.6
(1.7) 		644.5
(-3) 		1313

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1308
1 2 3 4 5 6 range
A 1381.4
(2.3)
P 		1500.6
(3)
P 		1296
(1.8)
P 		955.3
(-0.3)
P 		610.5
(-2.4)
P 		516.7
(-3)
P 		984
B 1751.9
(2.7)
P 		1790.2
(2.8)
P 		1824.8
(3)
P 		839
(-1.6)
P 		715.8
(-2.2)
P 		542
(-3)
P 		1283
C 1485.9
(1.9)
P 		1686.6
(3)
P 		1228.5
(0.5)
P 		810
(-1.9)
P 		714.6
(-2.4)
P 		606.6
(-3)
P 		1080
avr 1539.7
(2.4) 		1659.1
(3) 		1449.8
(1.9) 		868.1
(-1.3) 		680.3
(-2.3) 		555.1
(-3) 		1104
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 616
1 2 3 4 range
A 716.7
(3)
P 		325.9
(-0.8)
P 		388.8
(-0.2)
P 		100.7
(-3)
P 		616
B 614.8
(3)
P 		404.3
(0.4)
P 		393.4
(0.2)
P 		132.7
(-3)
P 		482
C 654.5
(3)
P 		334
(-0.5)
P 		643
(2.9)
P 		105
(-3)
P 		550
avr 662
(3) 		354.7
(-0.4) 		475.1
(1) 		112.8
(-3) 		549

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 484
1 2 3 4 5 6 range
A 254.6
(-1.1)
P 		533.7
(3)
P 		282.2
(-0.7)
P 		242.3
(-1.3)
P 		184.2
(-2.1)
P 		125.8
(-3)
P 		408
B 609.6
(3)
P 		595.9
(2.8)
P 		443.1
(0.5)
P 		222.2
(-2.7)
P 		290.8
(-1.7)
P 		202.1
(-3)
P 		408
C 550.8
(3)
P 		514.6
(2.4)
P 		312.7
(-0.7)
P 		162.9
(-3)
P 		231.2
(-1.9)
P 		203.1
(-2.4)
P 		388
avr 471.7
(1.8) 		548.1
(3) 		346
(-0.3) 		209.1
(-2.5) 		235.4
(-2.1) 		177
(-3) 		371
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182487_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4173
Sequence Type Exemplar sequence
Representative Public ID EGAP2.3 NCBI
Target Description EGAP2.3 /REP_DB=WormBase Gene ID /WP=CE04325 /TR=Q19076 /GB=AAA83593.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062582 NCBI Caenorhabditis elegans intestinal acid PHOsphatase family member (pho-1) (pho-1) mRNA, complete cds. 11/11 A 
SNAP00000035698 ENSEMBL cdna:SNAP chromosome:CEL140:II:5233279:5235808:-1 11/11 None
GENEFINDER00000035700 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5233279:5235808:-1 11/11 None
EGAP2.3.1 ENSEMBL cdna:known chromosome:CEL140:II:5233134:5235822:-1 gene:EGAP2.3 11/11 None
EGAP2.3.2 ENSEMBL cdna:known chromosome:CEL140:II:5233134:5235902:-1 gene:EGAP2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5233276-5235806(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pho-1
Ensembl EGAP2.3
Entrez Gene 173896 Entrez gene
SwissProt Q19076 EMBL-EBI
WormBase CE04325 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3993 acid phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83593 Intestinal acid phosphatase protein 1 [Caenorhabditis elegans] ref|NP_494983.1| intestinal acid PHOsphatase family member (pho-1) [Caenorhabditis elegans] 0.0
blast CAE59070 Hypothetical protein CBG02356 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 3.9E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494983.1 1 426-448
EGAP2.3.1 1 426-448
EGAP2.3.2 1 426-448
SNAP00000035698 1 429-451
GENEFINDER00000035700 1 426-448
Sequence
Target Sequence 
>C. ELEGANS:182487_S_AT
ctgttccaaacattgtcaacggacatgatgtaggagtgttaactcgaaaggtcagaggtg
gaccaattttgaatgatatggttatgcatataaatctcaaattgatgtgccagggtcaga
caactccaaattgcacatggattaataatttgaagaattacatttactctgctcacgaca
ccacaatctacgccttcttctccgcacttctaattgaagaatacgctgtaaaaccgtctg
gtggatatccattgtattctgctgcagtccttttagagctttacattgatagtgtcgaca
agaaaccatacttcaaaatggtatatcacgagcaagacggaagtggattcaaagatgtga
ctatgggaattcaaggatgtcctcaaaatagttcatactgtgatttagatattctcagaa
actttgccaatactatcaagccagatcaaccaatcgatcagtggtgcttaaccgatttga
acaagagttccagctttgctacagtctcaatgctctttattgcagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTTCCAAACATTGTCAACGGACA 376 239 797 Antisense
GGAGTGTTAACTCGAAAGGTCAGAG 483 515 829 Antisense
GTCAGAGGTGGACCAATTTTGAATG 563 463 847 Antisense
AATCTCAAATTGATGTGCCAGGGTC 398 169 889 Antisense
GATGTGCCAGGGTCAGACAACTCCA 39 407 900 Antisense
GTCAGACAACTCCAAATTGCACATG 696 463 911 Antisense
TTCTTCTCCGCACTTCTAATTGAAG 79 691 991 Antisense
GAATACGCTGTAAAACCGTCTGGTG 2 327 1015 Antisense
AAAACCGTCTGGTGGATATCCATTG 384 131 1026 Antisense
TATCCATTGTATTCTGCTGCAGTCC 68 659 1042 Antisense
GTCTCAATGCTCTTTATTGCAGCAA 96 469 1300 Antisense
Sequence Source Affymetrix Proprietary Database