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[182486_at]

Description E04F6.8 /REP_DB=WormBase Gene ID /WP=CE01220 /TR=Q19063 /GB=AAA68363.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 56      6grp_total > 71      4grp_total > 03      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4656
1 2 3 4 range
A 1482.1
(-1.7)
P 		644.2
(-3)
P 		760.3
(-2.8)
P 		4529.3
(3)
P 		3885
B 1297.9
(-1.9)
P 		547.8
(-3)
P 		723.9
(-2.7)
P 		4646.4
(3)
P 		4099
C 1311.3
(-2)
P 		538.6
(-3)
P 		745.1
(-2.7)
P 		5194.4
(3)
P 		4656
avr 1363.8
(-1.9) 		576.9
(-3) 		743.1
(-2.8) 		4790
(3) 		4213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1431
1 2 3 4 5 6 range
A 1355.2
(-3)
P 		1643.7
(-1.8)
P 		2786.2
(3)
P 		2614.2
(2.3)
P 		1677.4
(-1.6)
P 		1512.7
(-2.3)
P 		1431
B 2302.4
(0.7)
P 		2143.8
(-0.5)
P 		2188.9
(-0.2)
P 		1993.7
(-1.7)
P 		2609.5
(3)
P 		1819.9
(-3)
P 		790
C 2230.5
(3)
P 		1776.2
(-0.5)
P 		1451.3
(-3)
P 		2016.5
(1.3)
P 		2233.3
(3)
P 		1864.7
(0.2)
P 		782
avr 1962.7
(-0.1) 		1854.6
(-1.5) 		2142.1
(2.2) 		2208.1
(3) 		2173.4
(2.6) 		1732.4
(-3) 		476
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 855
1 2 3 4 range
A 539.6
(1.6)
P 		139.8
(-3)
P 		198.8
(-2.3)
P 		663
(3)
P 		523
B 413.9
(-1)
P 		135.3
(-3)
P 		167.7
(-2.8)
P 		990.1
(3)
P 		855
C 434.1
(-0.7)
P 		154.9
(-3)
P 		259.7
(-2.1)
P 		875.8
(3)
P 		721
avr 462.5
(-0.3) 		143.3
(-3) 		208.7
(-2.4) 		843
(3) 		700

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 810
1 2 3 4 5 6 range
A 249.8
(-3)
P 		584.6
(1.9)
P 		606.8
(2.2)
P 		663
(3)
P 		506.2
(0.7)
P 		368.3
(-1.3)
P 		413
B 801.1
(0.1)
P 		713.6
(-0.9)
P 		531.5
(-3)
P 		528.1
(-3)
P 		1060.2
(3)
P 		678.6
(-1.3)
P 		532
C 826.8
(3)
P 		542
(-0.7)
P 		369.4
(-3)
P 		405.5
(-2.5)
P 		722.7
(1.6)
P 		624.2
(0.3)
P 		457
avr 625.9
(-0.2) 		613.4
(-0.4) 		502.6
(-3) 		532.2
(-2.3) 		763
(3) 		557
(-1.7) 		260
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182486_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5365
Sequence Type Exemplar sequence
Representative Public ID E04F6.8 NCBI
Target Description E04F6.8 /REP_DB=WormBase Gene ID /WP=CE01220 /TR=Q19063 /GB=AAA68363.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063101(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063101 NCBI Caenorhabditis elegans E04F6.8 (E04F6.8) mRNA, complete cds. 11/11 None
SNAP00000032899 ENSEMBL cdna:SNAP chromosome:CEL140:II:7210729:7211310:-1 11/11 None
GENEFINDER00000032911 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7210729:7211310:-1 11/11 None
E04F6.8.1 ENSEMBL cdna:known chromosome:CEL140:II:7210690:7211311:-1 gene:E04F6.8 11/11 None
E04F6.8.2 ENSEMBL cdna:known chromosome:CEL140:II:7210717:7211313:-1 gene:E04F6.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7210724-7211306(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04F6.8
Entrez Gene 174184 Entrez gene
SwissProt Q19063 EMBL-EBI
WormBase CE01220 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68363 Hypothetical protein E04F6.8 [Caenorhabditis elegans] ref|NP_495502.1| E04F6.8 [Caenorhabditis elegans] 3.0E-66
blast CAE67571 Hypothetical protein CBG13099 [Caenorhabditis briggsae] 2.0E-43
Sequence
Target Sequence 
>C. ELEGANS:182486_AT
gtttttgatttcttcgtttatactgattgcctttgttcttcagtttggagaaggatcaat
tgcagttcaagaagttaaggatggcgaaaaagtgcaaattgaacttttcaaaggagccaa
ggcaatccagagatccgttgacgctggcgaacagattttccatttcgaaggagaaaacaa
aggagtgtttgtggatgctaatggaaaagctattgactcgtcaaattatgaagagaataa
cggacatttggtcatcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTTGATTTCTTCGTTTATACTG 359 449 21 Antisense
GTTTATACTGATTGCCTTTGTTCTT 640 447 36 Antisense
ATTGCCTTTGTTCTTCAGTTTGGAG 130 7 46 Antisense
GAGAAGGATCAATTGCAGTTCAAGA 336 391 68 Antisense
GAACTTTTCAAAGGAGCCAAGGCAA 648 349 121 Antisense
GGCAATCCAGAGATCCGTTGACGCT 46 535 141 Antisense
GATCCGTTGACGCTGGCGAACAGAT 478 415 152 Antisense
GGCGAACAGATTTTCCATTTCGAAG 46 541 166 Antisense
GGAGTGTTTGTGGATGCTAATGGAA 452 515 202 Antisense
AATGGAAAAGCTATTGACTCGTCAA 264 161 220 Antisense
GAATAACGGACATTTGGTCATCAAA 109 327 255 Antisense
Sequence Source Affymetrix Proprietary Database