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[182484_at]

Description F35C11.1 /REP_DB=WormBase Gene ID /WP=CE02208 /TR=Q20017 /GB=CAA90240.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 51      6grp_total > 24      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 749
1 2 3 4 range
A 840.6
(3)
P 		392.3
(-1.6)
P 		258.4
(-3)
P 		627.5
(0.8)
P 		582
B 978.7
(3)
P 		429.3
(-1.7)
P 		280.6
(-3)
P 		714.1
(0.7)
P 		698
C 967
(3)
P 		365.4
(-1.9)
P 		229.3
(-3)
P 		712.3
(0.9)
P 		738
avr 928.8
(3) 		395.7
(-1.8) 		256.1
(-3) 		684.6
(0.8) 		673

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 486
1 2 3 4 5 6 range
A 726.4
(3)
P 		600.3
(0.8)
P 		692.8
(2.4)
P 		492.7
(-1.1)
P 		393.2
(-2.8)
P 		382.7
(-3)
P 		344
B 663.8
(1.1)
P 		787.1
(3)
P 		641.3
(0.7)
P 		557.7
(-0.6)
P 		431.2
(-2.6)
P 		406.8
(-3)
P 		380
C 601.5
(0.4)
P 		811.4
(3)
P 		459.1
(-1.4)
P 		575.1
(0.1)
P 		325.5
(-3)
P 		481.6
(-1.1)
P 		486
avr 663.9
(1.8) 		732.9
(3) 		597.7
(0.7) 		541.8
(-0.3) 		383.3
(-3) 		423.7
(-2.3) 		350
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 306
(3)
P 		85.2
(-2.6)
P 		67.5
(-3)
P 		91.9
(-2.4)
P 		239
B 312.1
(3)
P 		106
(-2)
P 		65
(-3)
P 		152.2
(-0.9)
P 		247
C 320.1
(3)
P 		105.1
(-2.4)
P 		79.9
(-3)
P 		120.1
(-2)
P 		240
avr 312.7
(3) 		98.8
(-2.3) 		70.8
(-3) 		121.4
(-1.7) 		242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 133.9
(-1)
P 		213.5
(3)
P 		150.9
(-0.1)
P 		125
(-1.4)
P 		118.7
(-1.7)
P 		93.2
(-3)
P 		120
B 231
(1.4)
P 		262
(3)
P 		155.7
(-2.6)
P 		147.7
(-3)
P 		175.2
(-1.6)
P 		151.7
(-2.8)
P 		114
C 222.9
(2)
P 		247.6
(3)
P 		116.9
(-2.5)
P 		115.7
(-2.6)
P 		105.3
(-3)
P 		161.2
(-0.6)
P 		142
avr 195.9
(0.6) 		241
(3) 		141.2
(-2.4) 		129.5
(-3) 		133.1
(-2.8) 		135.4
(-2.7) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182484_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2874
Sequence Type Exemplar sequence
Representative Public ID F35C11.1 NCBI
Target Description F35C11.1 /REP_DB=WormBase Gene ID /WP=CE02208 /TR=Q20017 /GB=CAA90240.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063334(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063334 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-5) (nlp-5) mRNA, complete cds. 11/11 None
SNAP00000018021 ENSEMBL cdna:SNAP chromosome:CEL140:II:8242460:8243613:1 11/11 None
GENEFINDER00000018029 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8242460:8243613:1 11/11 None
F35C11.1 ENSEMBL cdna:known chromosome:CEL140:II:8242430:8243687:1 gene:F35C11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8242456-8243610(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-5
Ensembl F35C11.1
Entrez Gene 185270 Entrez gene
SwissProt Q20017 EMBL-EBI
WormBase CE02208 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90240 Hypothetical protein F35C11.1 [Caenorhabditis elegans] ref|NP_495735.1| Neuropeptide-Like Protein family member (nlp-5) [Caenorhabditis elegans] 5.0E-62
blast CAE57665 Hypothetical protein CBG00657 [Caenorhabditis briggsae] 3.0E-42
Sequence
Target Sequence 
>C. ELEGANS:182484_AT
ggaattgccaatattgcagcatcttttgtggtttcctcggtcagattccaatcagctccg
atgcgcgcgttgatagaaattaaccgagaacttgcaaaacgatcagtctcacaactgaat
cagtacgccggttttgacactcttggaggaatgggtctcggaaagcgaagtgaaccagat
caagcaggagagaaaagagcattgagcacttttgattcacttggaggaatgggactcggt
aaacgatcatcatcatcttcgagagtttttgtgtatgacaagcgtgccctccagcacttt
agctcattgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATTGCCAATATTGCAGCATCTT 628 533 40 Antisense
TTGCAGCATCTTTTGTGGTTTCCTC 594 703 53 Antisense
TGGTTTCCTCGGTCAGATTCCAATC 505 551 68 Antisense
CTCCGATGCGCGCGTTGATAGAAAT 139 235 95 Antisense
GAACTTGCAAAACGATCAGTCTCAC 216 351 127 Antisense
GAATCAGTACGCCGGTTTTGACACT 532 327 156 Antisense
GACACTCTTGGAGGAATGGGTCTCG 260 365 175 Antisense
GAGCATTGAGCACTTTTGATTCACT 398 389 236 Antisense
GGGACTCGGTAAACGATCATCATCA 28 495 270 Antisense
TTTGTGTATGACAAGCGTGCCCTCC 350 667 307 Antisense
CCCTCCAGCACTTTAGCTCATTGGA 1 261 326 Antisense
Sequence Source Affymetrix Proprietary Database