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[182474_at]

Description Y59A8B.6 /REP_DB=WormBase Gene ID /WP=CE26839 /TR=Q9GRZ2 /GB=CAC14407.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 41      6grp_total > 06      4grp_total > 55      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 406
1 2 3 4 range
A 848.2
(3)
P 		810.1
(1.4)
P 		701
(-3)
P 		760.2
(-0.6)
P 		147
B 846.9
(3)
P 		758
(0.1)
P 		823.4
(2.2)
P 		664.7
(-3)
P 		182
C 914.2
(3)
P 		508.2
(-3)
P 		715.8
(0.1)
P 		737.3
(0.4)
P 		406
avr 869.8
(3) 		692.1
(-3) 		746.7
(-1.2) 		720.7
(-2) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 550
1 2 3 4 5 6 range
A 1040.6
(3)
P 		719.1
(-0.5)
P 		572.7
(-2.1)
P 		490.5
(-3)
P 		503.1
(-2.9)
P 		610.7
(-1.7)
P 		550
B 931.7
(3)
P 		733.8
(0.2)
P 		623.5
(-1.4)
P 		707.3
(-0.2)
P 		651.8
(-1)
P 		511
(-3)
P 		421
C 707.8
(2.7)
P 		718.2
(3)
P 		653
(1.1)
P 		688.9
(2.1)
P 		577
(-1.2)
P 		515.8
(-3)
P 		202
avr 893.4
(3) 		723.7
(0.1) 		616.4
(-1.8) 		628.9
(-1.6) 		577.3
(-2.5) 		545.8
(-3) 		348
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 308.8
(3)
P 		175.8
(-1)
P 		183.3
(-0.8)
P 		111.3
(-3)
P 		198
B 270.1
(3)
P 		187.2
(-0.9)
P 		190.8
(-0.7)
P 		141.6
(-3)
P 		129
C 302.6
(3)
P 		146.1
(-2.3)
P 		249.5
(1.2)
P 		124.3
(-3)
P 		178
avr 293.8
(3) 		169.7
(-1.4) 		207.9
(-0.1) 		125.7
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 191.8
(0.1)
P 		255.7
(3)
P 		124.7
(-3)
P 		124.4
(-3)
P 		151.8
(-1.7)
P 		148.7
(-1.9)
P 		131
B 324.2
(3)
P 		244.2
(0.2)
P 		151.4
(-3)
P 		187.3
(-1.8)
P 		264.8
(0.9)
P 		190.5
(-1.6)
P 		173
C 262.3
(3)
P 		219.1
(0.9)
P 		166.2
(-1.7)
P 		138.5
(-3)
P 		186.7
(-0.7)
P 		172.7
(-1.3)
P 		124
avr 259.4
(3) 		239.7
(1.9) 		147.4
(-3) 		150.1
(-2.9) 		201.1
(-0.1) 		170.6
(-1.8) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182474_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14780
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.6 NCBI
Target Description Y59A8B.6 /REP_DB=WormBase Gene ID /WP=CE26839 /TR=Q9GRZ2 /GB=CAC14407.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075124(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075124 NCBI Caenorhabditis elegans Y59A8B.6 (Y59A8B.6) mRNA, complete cds. 9/11 None
SNAP00000028701 ENSEMBL cdna:SNAP chromosome:CEL140:V:18035060:18054897:1 9/11 None
GENEFINDER00000028725 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18025061:18044799:1 9/11 None
Y59A8B.6.2 ENSEMBL cdna:known chromosome:CEL140:V:18023439:18044891:1 gene:Y59A8B.6 9/11 None
Y59A8B.6.1 ENSEMBL cdna:known chromosome:CEL140:V:18025053:18044928:1 gene:Y59A8B.6 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18025060-18044799(+) 95.52 100.0
Public Domain and Genome References
Gene Title
Ensembl Y59A8B.6
Entrez Gene 180180 Entrez gene
SwissProt Q9GRZ2 EMBL-EBI
WormBase CE28858 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13575_AT pre-mRNA splicing factor-related at
ATH1-121501:255382_AT pre-mRNA splicing factor-related at
CANINE_2:CFA.20738.1.S1_S_AT similar to U5 snRNP-associated 102 kDa protein cfa
CANINE_2:CFAAFFX.20397.1.S1_S_AT similar to U5 snRNP-associated 102 kDa protein cfa
DROSOPHILA_2:1628665_AT dm
DROSGENOME1:155017_AT dm
CHICKEN:GGAAFFX.26697.1.S1_AT similar to RIKEN cDNA 1190003A07 gga
CHICKEN:GGAAFFX.26697.2.S1_S_AT similar to RIKEN cDNA 1190003A07 gga
HG-U133A:208879_X_AT chromosome 20 open reading frame 14 hs
HG-U133A_2:208879_X_AT chromosome 20 open reading frame 14 hs
HG-U133_PLUS_2:208879_X_AT chromosome 20 open reading frame 14 hs
HG-FOCUS:208880_S_AT chromosome 20 open reading frame 14 hs
HG-U133A_2:208880_S_AT chromosome 20 open reading frame 14 hs
HG-U133A:208880_S_AT chromosome 20 open reading frame 14 hs
HG-U133_PLUS_2:208880_S_AT chromosome 20 open reading frame 14 hs
HU35KSUBA:AA210846_AT chromosome 20 open reading frame 14 hs
HU35KSUBA:RC_AA070841_AT chromosome 20 open reading frame 14 hs
HU35KSUBA:RC_AA425637_AT chromosome 20 open reading frame 14 hs
HU35KSUBC:RC_AA121962_S_AT chromosome 20 open reading frame 14 hs
U133_X3P:G4164165_3P_A_AT chromosome 20 open reading frame 14 hs
U133_X3P:HS.31334.0.S1_3P_A_AT chromosome 20 open reading frame 14 hs
HG-U95AV2:34345_AT chromosome 20 open reading frame 14 hs
HG-U95C:63714_F_AT chromosome 20 open reading frame 14 hs
HG-U95D:73609_I_AT Chromosome 20 open reading frame 14 hs
MOE430A:1426026_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430A_2:1426026_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430_2:1426026_AT RIKEN cDNA 2610031L17 gene mm
MOE430A:1454789_X_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430A_2:1454789_X_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430_2:1454789_X_AT RIKEN cDNA 2610031L17 gene mm
MU11KSUBA:AA437494_AT RIKEN cDNA 2610031L17 gene mm
MU11KSUBB:MSA.39147.0_S_AT RIKEN cDNA 2610031L17 gene mm
MG-U74AV2:101053_AT RIKEN cDNA 2610031L17 gene mm
MOE430A:1424036_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430A_2:1424036_AT RIKEN cDNA 2610031L17 gene mm
MOUSE430_2:1424036_AT RIKEN cDNA 2610031L17 gene mm
MU19KSUBB:TC33523_AT RIKEN cDNA 2610031L17 gene, mRNA (cDNA clone MGC:38351 IMAGE:5344332) mm
MU19KSUBB:TC33523_G_AT RIKEN cDNA 2610031L17 gene, mRNA (cDNA clone MGC:38351 IMAGE:5344332) mm
MU19KSUBC:TC41546_AT RIKEN cDNA 2610031L17 gene, mRNA (cDNA clone MGC:38351 IMAGE:5344332) mm
RG-U34C:RC_AA819400_AT similar to RIKEN cDNA 1190003A07 (predicted) rn
RAE230A:1389629_AT similar to RIKEN cDNA 1190003A07 (predicted) rn
RAT230_2:1389629_AT similar to RIKEN cDNA 1190003A07 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6396 RNA processing inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC14407 Hypothetical protein Y59A8B.6 [Caenorhabditis elegans] ref|NP_507525.1| Y59A8B.6 [Caenorhabditis elegans] 0.0
blast CAE61660 Hypothetical protein CBG05596 [Caenorhabditis briggsae] 0.0
blast XP_534481 PREDICTED: similar to U5 snRNP-associated 102 kDa protein [Canis familiaris] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010491 EMBL-EBI PRP1 splicing factor, N-terminal 4.6E-98
Pfam IPR004953 EMBL-EBI EB1 protein 2.8E-27
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-9
Pfam IPR010491 EMBL-EBI PRP1 splicing factor, N-terminal 5.1E-45
Pfam IPR010491 EMBL-EBI PRP1 splicing factor, N-terminal 3.0E-28
Sequence
Target Sequence 
>C. ELEGANS:182474_AT
aagtgtccgttttgagcagcgtgtcggatgtcctgaaatggcaaaagaacgaatgagtcg
agcacttcaagagtgtgaaggaagtgtaacaccttgtgaaccaaaatggacgattttgag
gctgatacgcaaaaactgtccgagttgtactctaattttgccaattttgacttgttatac
caagtctccccagaagtcgggggccgcgccggaggtgcggtccacggctggaaagctatg
ggctgaagcaatttggatggaaggaccacacggccgacgtgctaaatcgattgatgcatt
gaaaaagtgtgaacacaatccacacgtacttattgcggctgcaaggcttttctggagtga
gagaaagatcaaaaaggcacgtgaatggtttgttagagctgttaatctggatccggataa
tggagacgcattcgccaattttttggctttcgagcagattcacggaaaagaggaggaccg
caagtcggtattcaaaaaatgtgtgacatcggagcctcgttacggtgatctatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTGTCCGTTTTGAGCAGCGTGTC 481 159 2601 Antisense
GCAGCGTGTCGGATGTCCTGAAATG 59 315 2616 Antisense
CTTGTTATACCAAGTCTCCCCAGAA 604 215 2771 Antisense
GTCCACGGCTGGAAAGCTATGGGCT 355 475 2820 Antisense
ACCACACGGCCGACGTGCTAAATCG 36 85 2865 Antisense
ATCCACACGTACTTATTGCGGCTGC 245 41 2918 Antisense
TATTGCGGCTGCAAGGCTTTTCTGG 529 663 2931 Antisense
ATGGAGACGCATTCGCCAATTTTTT 685 51 3020 Antisense
TTGGCTTTCGAGCAGATTCACGGAA 539 707 3043 Antisense
AAATGTGTGACATCGGAGCCTCGTT 72 121 3097 Antisense
GGAGCCTCGTTACGGTGATCTATGG 448 521 3111 Antisense
Sequence Source Affymetrix Proprietary Database