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[182468_s_at]

Description K05F1.6 /REP_DB=WormBase Gene ID /WP=CE02810 /TR=Q21240 /GB=AAA68713.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 50      6grp_total > 15      4grp_total > 39      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 559
1 2 3 4 range
A 681
(3)
P 		256.2
(-2.1)
P 		182.3
(-3)
P 		331.1
(-1.2)
P 		499
B 598.5
(3)
P 		318.4
(-0.8)
P 		156.8
(-3)
P 		388.3
(0.1)
P 		442
C 716.1
(3)
P 		257.7
(-2.6)
P 		224.6
(-3)
P 		399.8
(-0.9)
P 		492
avr 665.2
(3) 		277.4
(-1.9) 		187.9
(-3) 		373.1
(-0.7) 		477

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 527
1 2 3 4 5 6 range
A 654.9
(3)
P 		605.9
(2.1)
P 		335.3
(-2.8)
P 		382.1
(-1.9)
P 		408.6
(-1.5)
P 		323.4
(-3)
P 		332
B 517.4
(-0.2)
P 		761.6
(3)
P 		343.8
(-2.5)
P 		305.7
(-3)
P 		466.2
(-0.9)
P 		364.1
(-2.2)
P 		456
C 833
(3)
P 		713.8
(1.4)
P 		386.4
(-3)
P 		463.8
(-1.9)
P 		394.4
(-2.9)
P 		383.2
(-3)
P 		450
avr 668.4
(2.5) 		693.8
(3) 		355.2
(-3) 		383.9
(-2.5) 		423.1
(-1.8) 		356.9
(-3) 		339
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 247.9
(3)
P 		55.6
(-2.8)
P 		47.7
(-3)
P 		48.5
(-3)
P 		200
B 190.9
(3)
P 		78.6
(-1.4)
P 		36.3
(-3)
P 		82.7
(-1.2)
P 		155
C 237
(3)
P 		74.1
(-2.8)
P 		78.3
(-2.6)
P 		67.4
(-3)
P 		170
avr 225.3
(3) 		69.4
(-2.5) 		54.1
(-3) 		66.2
(-2.6) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 120.7
(-1)
P 		215.5
(3)
P 		73
(-3)
P 		96.9
(-2)
P 		123.3
(-0.9)
P 		78.7
(-2.8)
P 		143
B 180
(0.4)
P 		253.5
(3)
P 		83.5
(-2.9)
P 		81
(-3)
P 		189.4
(0.8)
P 		135.8
(-1.1)
P 		173
C 308.8
(3)
P 		217.8
(0.5)
P 		98.3
(-2.9)
P 		93.3
(-3)
P 		127.6
(-2)
P 		128.3
(-2)
P 		216
avr 203.2
(1.9) 		228.9
(3) 		84.9
(-3) 		90.4
(-2.8) 		146.8
(-0.4) 		114.3
(-1.8) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182468_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4623
Sequence Type Exemplar sequence
Representative Public ID K05F1.6 NCBI
Target Description K05F1.6 /REP_DB=WormBase Gene ID /WP=CE02810 /TR=Q21240 /GB=AAA68713.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027062(10), NM_001027063(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027062 NCBI Caenorhabditis elegans K05F1.6a (K05F1.6) mRNA, complete cds. 10/11 None
NM_001027063 NCBI Caenorhabditis elegans K05F1.6b (K05F1.6) mRNA, complete cds. 10/11 None
SNAP00000031240 ENSEMBL cdna:SNAP chromosome:CEL140:II:5783686:5787271:1 10/11 None
GENEFINDER00000031255 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5783686:5787315:1 11/11 None
K05F1.6a ENSEMBL cdna:known chromosome:CEL140:II:5782913:5787620:1 gene:K05F1.6 10/11 None
K05F1.6b ENSEMBL cdna:known chromosome:CEL140:II:5784243:5787271:1 gene:K05F1.6 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5783682-5787312(+) 100.0 100.0
Public Domain and Genome References
Ensembl K05F1.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022233 K05F1.6a [Caenorhabditis elegans] 0.0
blast AAZ94294 Hypothetical protein K05F1.6a [Caenorhabditis elegans] 0.0
blast CAE59188 Hypothetical protein CBG02498 [Caenorhabditis briggsae] 0.0
blast AAA68713 Hypothetical protein K05F1.6b [Caenorhabditis elegans] ref|NP_001022234.1| K05F1.6b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 3.4E-10
Pfam IPR005828 EMBL-EBI General substrate transporter 3.4E-10
Pfam IPR005828 EMBL-EBI General substrate transporter 3.4E-10
Pfam IPR005828 EMBL-EBI General substrate transporter 3.4E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022233.1 11 359-378,385-404,409-431,444-466,471-490,568-590,605-627,634-656,660-682,694-716,721-743
NP_001022234.1 10 131-148,158-175,215-237,242-261,339-361,376-398,405-427,431-453,465-487,492-514
K05F1.6a 11 359-378,385-404,409-431,444-466,471-490,568-590,605-627,634-656,660-682,694-716,721-743
K05F1.6b 10 131-148,158-175,215-237,242-261,339-361,376-398,405-427,431-453,465-487,492-514
SNAP00000031240 11 260-279,286-305,310-332,345-367,372-391,469-491,506-528,535-557,561-583,595-617,622-644
GENEFINDER00000031255 11 272-291,298-317,322-344,357-379,384-403,481-503,518-540,547-569,573-595,607-629,634-656
Sequence
Target Sequence 
>C. ELEGANS:182468_S_AT
acttctggtttgttcatcattgttgcaatgctctcaatggtttctggccactacatgtta
gctctggcattcatgtattttggaaagatagctgtacaaggagcatttaatattctctat
atatttacaagtgaactatatccaactgttgttcgtaacactgcagttggtgtcacttca
atggtggctcgatttggatctggattatcgtcctacattgctctgttgtcaaacatttct
ctaccaatcgttccaatgattattttcgctgtgttctcattgtttgctggaatgttggtg
ttcgtattaccagaaactagtgagaagccactaccggaaacattagatgatgctatcaac
ttcctggaacctaccaaacaatttaaaaccattgagtcgacgatcgtcggctataacaag
aaggatgacggaacgggcaagcgcgagtcgtacaaattcaatagtacaagatattcaaga
tattcaaattccatcggacttcaaaatgcccgatttggtatccaccgacgagtaacattt
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTCTGGTTTGTTCATCATTGTTG 6 105 1672 Antisense
GCAATGCTCTCAATGGTTTCTGGCC 604 321 1696 Antisense
TTCTGGCCACTACATGTTAGCTCTG 120 693 1713 Antisense
GGTGTCACTTCAATGGTGGCTCGAT 586 499 1840 Antisense
GGATCTGGATTATCGTCCTACATTG 302 511 1867 Antisense
GTCCTACATTGCTCTGTTGTCAAAC 167 477 1881 Antisense
CAAACATTTCTCTACCAATCGTTCC 565 191 1901 Antisense
ATTTTCGCTGTGTTCTCATTGTTTG 258 15 1933 Antisense
TGATGCTATCAACTTCCTGGAACCT 273 565 2019 Antisense
ACGGAACGGGCAAGCGCGAGTCGTA 398 95 2099 Antisense
GGTATCCACCGACGAGTAACATTTC 464 505 2188 Antisense
Sequence Source Affymetrix Proprietary Database