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[182446_at]

Description F35B12.5 /REP_DB=WormBase Gene ID /WP=CE27758 /TR=Q20010 /GB=CAA98463.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 93      6grp_total > 77      4grp_total > 46      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 434.5
(3)
P 		344
(-0.1)
P 		259.6
(-3)
P 		300.8
(-1.6)
P 		175
B 415.4
(3)
P 		350
(0.1)
P 		296.1
(-2.4)
P 		282.3
(-3)
P 		133
C 383.1
(3)
P 		325.5
(-0.6)
P 		287.9
(-3)
P 		319.4
(-1)
P 		95
avr 411
(3) 		339.8
(-0.3) 		281.2
(-3) 		300.8
(-2.1) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 726
1 2 3 4 5 6 range
A 245.4
(-3)
P 		272.5
(-2.7)
P 		293
(-2.5)
P 		315.4
(-2.3)
P 		448.7
(-1.1)
P 		873.9
(3)
P 		629
B 282.9
(-2.2)
P 		246.7
(-2.6)
P 		267.2
(-2.4)
P 		213.1
(-3)
P 		443.1
(-0.5)
P 		766.9
(3)
P 		554
C 295.7
(-2.7)
P 		265.9
(-3)
P 		351.8
(-2.2)
P 		286.1
(-2.8)
P 		446
(-1.4)
P 		939.2
(3)
P 		673
avr 274.7
(-2.9) 		261.7
(-3) 		304
(-2.6) 		271.5
(-2.9) 		445.9
(-1.2) 		860
(3) 		598
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 158.2
(3)
P 		74.7
(-1.4)
P 		67.9
(-1.7)
P 		44
(-3)
P 		114
B 132.5
(3)
P 		86.4
(-0.8)
P 		68.6
(-2.3)
P 		60.2
(-3)
P 		72
C 126.8
(3)
P 		93.6
(0.3)
P 		100.3
(0.8)
P 		53.9
(-3)
P 		73
avr 139.2
(3) 		84.9
(-0.8) 		78.9
(-1.2) 		52.7
(-3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 45.2
(-3)
P 		96.9
(-1.1)
P 		63.8
(-2.3)
P 		80
(-1.8)
P 		135.4
(0.2)
P 		212.8
(3)
P 		168
B 98.4
(-1.9)
P 		82.1
(-2.3)
P 		64.9
(-2.8)
P 		56.4
(-3)
P 		180
(0.2)
P 		286
(3)
P 		230
C 109.6
(-1.8)
P 		81.1
(-2.4)
P 		89.5
(-2.3)
P 		57.5
(-3)
P 		144.3
(-1)
P 		314.4
(3)
P 		257
avr 84.4
(-2.4) 		86.7
(-2.4) 		72.7
(-2.8) 		64.6
(-3) 		153.2
(-0.4) 		271.1
(3) 		207
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182446_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14031
Sequence Type Exemplar sequence
Representative Public ID F35B12.5 NCBI
Target Description F35B12.5 /REP_DB=WormBase Gene ID /WP=CE27758 /TR=Q20010 /GB=CAA98463.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073543(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073543 NCBI Caenorhabditis elegans Spindle ASsembly abnormal family member (sas-5) (sas-5) mRNA, complete cds. 11/11 None
SNAP00000002153 ENSEMBL cdna:SNAP chromosome:CEL140:V:11613368:11615064:1 11/11 None
GENEFINDER00000002165 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11613368:11615064:1 11/11 None
F35B12.5 ENSEMBL cdna:known chromosome:CEL140:V:11613334:11615643:1 gene:F35B12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11613810-11615064(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sas-5
Ensembl F35B12.5
Entrez Gene 179592 Entrez gene
SwissProt Q20010 EMBL-EBI
WormBase CE27758 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98463 Hypothetical protein F35B12.5 [Caenorhabditis elegans] ref|NP_505944.1| Spindle ASsembly abnormal family member (sas-5) [Caenorhabditis elegans] sp|Q20010|SAS5_CAEEL Spindle assembly abnormal protein 0.0
blast Q60M48 Spindle assembly abnormal protein 5 emb|CAE75328.1| Hypothetical protein CBG23306 [Caenorhabditis briggsae] 1.0E-123
blast CAG28316 spindle assembly abnormal protein 5, SAS-5 [Ancylostoma ceylanicum] 1.0E-13
Sequence
Target Sequence 
>C. ELEGANS:182446_AT
gagagtcctcgtcaatcaagacagcagaagccagctagtaaaatcgcttcggcacgagaa
gttatcaaaagagacggtgttatacctccagaagcattaacaattattgagcaacgcctt
cgctcggacccaatgtttcgccaacaaattgataatgttctagcagacgctgaatgtgat
gctaacagagctgcatattctccaccaccaccaatgtctgaagttagatacggatctggt
gtgaatcctgctttgatgagagaaacacttacggtagaacgaagtattcgatatgacaac
ggactcgcttctattgattctagacagtggacaaatgagagacgtgataatcgtgctcct
gactcataccgtacctacgaaccggatcaaccatgtcactcactctatcaaaagggtcaa
agtattagctattatccgtcagaagcagctggaaaaacgacagctagaaacaatcgaact
ggatactacgtggaagattcatcagatcatgaagaggatgttgtagtcaataaacgggga
cagaactatcacgagcaagcggtacctgagactccagctgaacgagaacgccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGTCCTCGTCAATCAAGACAGC 494 393 256 Antisense
GAGACGGTGTTATACCTCCAGAAGC 483 391 326 Antisense
TTATTGAGCAACGCCTTCGCTCGGA 685 677 359 Antisense
GATCTGGTGTGAATCCTGCTTTGAT 560 415 488 Antisense
GATATGACAACGGACTCGCTTCTAT 29 425 545 Antisense
TGATAATCGTGCTCCTGACTCATAC 327 565 600 Antisense
TGACTCATACCGTACCTACGAACCG 381 569 615 Antisense
ACGAACCGGATCAACCATGTCACTC 702 91 632 Antisense
ACCATGTCACTCACTCTATCAAAAG 705 85 645 Antisense
ACGAGCAAGCGGTACCTGAGACTCC 575 93 806 Antisense
GACTCCAGCTGAACGAGAACGCCGT 599 373 825 Antisense
Sequence Source Affymetrix Proprietary Database