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[182440_s_at]

Description F35C8.7 /REP_DB=WormBase Gene ID /WP=CE04499 /TR=Q20026 /GB=AAA81709.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 69      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 81      6grp_total > 89      4grp_total > 46      6grp_high1_sub > Cu      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 295.7
(3)
P 		173
(-1.8)
P 		170.9
(-1.9)
P 		142.8
(-3)
P 		153
B 318.2
(3)
P 		226.5
(-1.3)
P 		189.5
(-3)
P 		206.3
(-2.2)
P 		129
C 268.8
(3)
P 		226.4
(0.1)
P 		180.8
(-3)
P 		213.8
(-0.8)
P 		88
avr 294.2
(3) 		208.6
(-1.5) 		180.4
(-3) 		187.6
(-2.6) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 155.9
(-3)
P 		326.6
(2.9)
P 		186.2
(-1.9)
P 		251.2
(0.3)
P 		321.7
(2.8)
P 		328.6
(3)
P 		173
B 244.6
(-0.3)
P 		305.6
(2.2)
P 		191.5
(-2.5)
P 		180.7
(-3)
P 		321.5
(2.9)
P 		324.4
(3)
P 		144
C 349.5
(1.1)
P 		333.8
(0.7)
P 		236.9
(-2)
P 		198.8
(-3)
P 		420.6
(3)
P 		299.9
(-0.3)
P 		222
avr 250
(-1.2) 		322
(1.7) 		204.9
(-3) 		210.2
(-2.8) 		354.6
(3) 		317.6
(1.5) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 107.7
(3)
P 		37.6
(-1.8)
P 		44.7
(-1.4)
P 		20.9
(-3)
P 		87
B 101.5
(3)
P 		56
(-1.7)
P 		43.9
(-3)
P 		44
(-3)
P 		58
C 89
(3)
P 		65.1
(0.3)
P 		63
(0.1)
P 		36
(-3)
P 		53
avr 99.4
(3) 		52.9
(-1.2) 		50.5
(-1.5) 		33.6
(-3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 28.7
(-3)
P 		116.1
(3)
P 		40.6
(-2.2)
P 		63.7
(-0.6)
P 		97.1
(1.7)
P 		80
(0.5)
P 		87
B 85.1
(-0.2)
P 		101.7
(0.9)
P 		46.5
(-3)
P 		47.9
(-2.9)
P 		130.6
(3)
P 		121
(2.3)
P 		84
C 129.6
(2.6)
P 		101.8
(0.9)
P 		60.3
(-1.7)
P 		40
(-3)
P 		136.1
(3)
P 		100.4
(0.8)
P 		96
avr 81.1
(-0.3) 		106.5
(1.8) 		49.1
(-3) 		50.5
(-2.9) 		121.3
(3) 		100.5
(1.3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182440_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16384
Sequence Type Exemplar sequence
Representative Public ID F35C8.7 NCBI
Target Description F35C8.7 /REP_DB=WormBase Gene ID /WP=CE04499 /TR=Q20026 /GB=AAA81709.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171686(11), NM_171685(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171686 NCBI Caenorhabditis elegans F35C8.7b (F35C8.7) mRNA, complete cds. 11/11 None
NM_171685 NCBI Caenorhabditis elegans F35C8.7a (F35C8.7) mRNA, complete cds. 11/11 None
GENEFINDER00000043846 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5355862:5363757:-1 11/11 None
SNAP00000043828 ENSEMBL cdna:SNAP chromosome:CEL140:X:5356022:5359899:-1 9/11 None
F35C8.7b ENSEMBL cdna:known chromosome:CEL140:X:5355603:5364287:-1 gene:F35C8.7 11/11 None
F35C8.7a.1 ENSEMBL cdna:known chromosome:CEL140:X:5355862:5363757:-1 gene:F35C8.7 11/11 None
F35C8.7a.2 ENSEMBL cdna:known chromosome:CEL140:X:5355862:5359912:-1 gene:F35C8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5355862-5359900(-) 100.0 100.0
Public Domain and Genome References
Ensembl F35C8.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM51502 Hypothetical protein F35C8.7b [Caenorhabditis elegans] ref|NP_741790.2| F35C8.7b [Caenorhabditis elegans] 0.0
blast AAA81709 Hypothetical protein F35C8.7a [Caenorhabditis elegans] ref|NP_741789.2| F35C8.7a [Caenorhabditis elegans] 0.0
blast CAE68742 Hypothetical protein CBG14673 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007603 EMBL-EBI Protein of unknown function DUF580 1.0E-126
Pfam IPR007603 EMBL-EBI Protein of unknown function DUF580 1.0E-126
Pfam IPR007603 EMBL-EBI Protein of unknown function DUF580 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741790.2 10 52-74,269-291,298-320,349-371,397-419,474-496,523-545,560-582,621-643,658-680
NP_741789.2 10 93-115,310-332,339-361,390-412,438-460,515-537,564-586,601-623,662-684,699-721
F35C8.7b 10 52-74,269-291,298-320,349-371,397-419,474-496,523-545,560-582,621-643,658-680
F35C8.7a.1 10 93-115,310-332,339-361,390-412,438-460,515-537,564-586,601-623,662-684,699-721
F35C8.7a.2 10 93-115,310-332,339-361,390-412,438-460,515-537,564-586,601-623,662-684,699-721
SNAP00000043828 10 93-115,310-332,339-361,390-412,438-460,515-537,564-586,601-623,662-684,699-721
Sequence
Target Sequence 
>C. ELEGANS:182440_S_AT
gatcatccgtgtacttcttgaatacattgaccacaagcttggcaagtcgcagaacaaagc
tgtcaagtggttcttgatgtgcttgaagtgttgtttctggtgtctggaagtgttcttcaa
attcctcactaaaaatgcctatattatgattgcaatttacggcaagaacttcttctcatc
agcaaaagattcattcttactgatcactagaaacattgtgagaaccgtcgtagttcacaa
ggtcgctggaatccttttattccttggaaagtccatgatcacacttggaatgggcattct
cagtttctactacttttccggacgatgggttgttgaaggtgttccgaaagtcgatctcta
ctactattttgtgccaattgtcattgtggtcattggcagttacttcatggccgacctgtt
ctttgatgtctacgaaatggctgtggatacaacgttcatttgcttcctggaagacagcga
acaaaacgatggaagccttgagcgaccgttcttcatgtccgaaaaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCATCCGTGTACTTCTTGAATAC 162 419 1752 Antisense
TGTTCTTCAAATTCCTCACTAAAAA 212 563 1862 Antisense
CGTCGTAGTTCACAAGGTCGCTGGA 225 245 1977 Antisense
AATGGGCATTCTCAGTTTCTACTAC 448 163 2040 Antisense
TTTCTACTACTTTTCCGGACGATGG 584 671 2055 Antisense
GGTGTTCCGAAAGTCGATCTCTACT 108 501 2089 Antisense
GATCTCTACTACTATTTTGTGCCAA 538 419 2104 Antisense
TTGGCAGTTACTTCATGGCCGACCT 27 707 2144 Antisense
CCGACCTGTTCTTTGATGTCTACGA 81 265 2162 Antisense
ACGATGGAAGCCTTGAGCGACCGTT 523 93 2237 Antisense
GACCGTTCTTCATGTCCGAAAAACT 666 199 2255 Antisense
Sequence Source Affymetrix Proprietary Database