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[182433_s_at]

Description C25D7.12 /REP_DB=WormBase Gene ID /WP=CE08397 /TR=O17603 /GB=CAB02938.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 35      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 7.1
(-2.9)
A 		75.3
(3)
A 		10.3
(-2.7)
A 		6.5
(-3)
A 		69
B 4.2
(-3)
A 		39.8
(3)
A 		22.1
(0)
A 		20.2
(-0.3)
A 		36
C 5.6
(-2.2)
A 		34.6
(2.9)
A 		1.1
(-3)
A 		35.4
(3)
A 		34
avr 5.6
(-3) 		49.9
(3) 		11.2
(-2.2) 		20.7
(-1) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 46
(-0.4)
A 		23.7
(-2.7)
A 		29.2
(-2.1)
A 		20.4
(-3)
A 		45.4
(-0.5)
A 		80
(3)
P 		60
B 2.7
(-2.9)
A 		5.1
(-2.6)
A 		1.5
(-3)
A 		18.8
(-1.2)
A 		48.3
(1.8)
A 		60.2
(3)
A 		59
C 28.7
(-1)
A 		36.1
(-0.4)
A 		5.8
(-3)
A 		14.1
(-2.3)
A 		42.4
(0.1)
A 		75.9
(3)
A 		70
avr 25.8
(-1.6) 		21.6
(-2.1) 		12.2
(-3) 		17.8
(-2.4) 		45.4
(0.3) 		72
(3) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.6
(-2.3)
A 		16.3
(3)
A 		2.7
(-2.3)
A 		0.9
(-3)
A 		15
B 1.3
(-3)
A 		9.8
(3)
A 		5.1
(-0.3)
A 		4.3
(-0.9)
A 		9
C 1.9
(-2.1)
A 		10
(3)
A 		0.4
(-3)
A 		6
(0.5)
A 		10
avr 1.9
(-3) 		12
(3) 		2.7
(-2.5) 		3.7
(-1.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 8.5
(-1.6)
A 		8.4
(-1.7)
A 		6.4
(-2.5)
A 		5.2
(-3)
A 		13.7
(0.6)
A 		19.5
(3)
P 		14
B 1
(-2.8)
A 		1.7
(-2.6)
A 		0.4
(-3)
A 		5
(-1.7)
A 		19.6
(2.2)
A 		22.4
(3)
A 		22
C 10.6
(-0.7)
A 		11
(-0.6)
A 		1.5
(-3)
A 		2.8
(-2.7)
A 		13.7
(0.1)
A 		25.4
(3)
A 		24
avr 6.7
(-1.8) 		7
(-1.7) 		2.8
(-3) 		4.3
(-2.5) 		15.7
(0.9) 		22.4
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182433_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13431
Sequence Type Exemplar sequence
Representative Public ID C25D7.12 NCBI
Target Description C25D7.12 /REP_DB=WormBase Gene ID /WP=CE08397 /TR=O17603 /GB=CAB02938.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074299(11), NM_074301(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074299 NCBI Caenorhabditis elegans C25D7.12 (C25D7.12) mRNA, complete cds. 11/11 None
NM_074301 NCBI Caenorhabditis elegans C25D7.2 (C25D7.2) mRNA, complete cds. 11/11 A 
SNAP00000019702 ENSEMBL cdna:SNAP chromosome:CEL140:V:15035168:15036444:1 10/11 None
SNAP00000019703 ENSEMBL cdna:SNAP chromosome:CEL140:V:15036966:15038006:1 11/11 None
SNAP00000019705 ENSEMBL cdna:SNAP chromosome:CEL140:V:15040381:15041421:-1 11/11 None
GENEFINDER00000019718 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15040381:15055800:-1 11/11 None
GENEFINDER00000019725 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15035425:15035937:1 11/11 None
GENEFINDER00000019726 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15036027:15038006:1 11/11 None
C25D7.12 ENSEMBL cdna:known chromosome:CEL140:V:15034756:15035937:1 gene:C25D7.12 11/11 None
C25D7.14 ENSEMBL cdna:pseudogene chromosome:CEL140:V:15037023:15038006:1 gene:C25D7.14 11/11 A 
C25D7.2 ENSEMBL cdna:known chromosome:CEL140:V:15040381:15041421:-1 gene:C25D7.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15034755-15035937(+) 100.0 100.0
chrV:15036986-15037982(+) 80.6 80.6
chrV:15040404-15041400(-) 80.6 80.6
Public Domain and Genome References
Gene Title

Ensembl C25D7.12
C25D7.14
C25D7.2
Entrez Gene 180005 Entrez gene
180008 Entrez gene
SwissProt O17603 EMBL-EBI
O17595 EMBL-EBI
WormBase CE08397 Wormbase
CE08388 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02774 Hypothetical protein C25D7.3 [Caenorhabditis elegans] ref|NP_506703.1| Sex determination and Dosage Compensation defect family member (sdc-3) [Caenorhabditis elegans] sp|P34706|SDC3_CAEEL Zinc finger protein sdc-3 0.0
blast AAB25348 Sdc-3=sex determination and dosage compensation control [Caenorhabditis elegans, Peptide, 2150 aa] gb|AAA28144.1| zinc finger protein 0.0
blast CAB02775 Hypothetical protein C25D7.4 [Caenorhabditis elegans] ref|NP_506704.1| C25D7.4 [Caenorhabditis elegans] 1.0E-143
blast CAB02938 Hypothetical protein C25D7.12 [Caenorhabditis elegans] emb|CAB02780.1| Hypothetical protein C25D7.12 [Caenorhabditis elegans] ref|NP_506700.1| C25D7.12 [Caenorhabditis elegans] 1.0E-116
blast CAB02773 Hypothetical protein C25D7.2 [Caenorhabditis elegans] ref|NP_506702.1| C25D7.2 [Caenorhabditis elegans] 2.0E-98
blast CAB02773 Hypothetical protein C25D7.2 [Caenorhabditis elegans] ref|NP_506702.1| C25D7.2 [Caenorhabditis elegans] 1.0E-97
blast AAB25348 Sdc-3=sex determination and dosage compensation control [Caenorhabditis elegans, Peptide, 2150 aa] gb|AAA28144.1| zinc finger protein 3.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 2.5E-27
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.2E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.5E-10
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 3.4E-7
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Sequence
Target Sequence 
>C. ELEGANS:182433_S_AT
cgaaggctctcaaattggttcccaatcagtcgaactacacattcccggagggacagggga
ctgcctcagtgaagacatccacccttcgtgcttcacccaacaagctgccattcgcaacaa
cgggtggtgttcagacagttagcatcgcgaacaacaccaagtcgagaaaggcgtttaagg
tcaaaacctctgacaatcttctgtaccgtgtcaacccagtctttggattcgttgagccgg
gagacaagctttcgatcgatgtgctccgtcacaatggtgttgagaagactgatcacatga
ttgtcttaacatccaatgcttcatcagaacaaaattgtgccaaaggagtcttcgagagtg
accaaccacgcgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAGGCTCTCAAATTGGTTCCCAA 10 251 605 Antisense
CCAATCAGTCGAACTACACATTCCC 197 275 626 Antisense
GACTGCCTCAGTGAAGACATCCACC 486 375 663 Antisense
CAACAAGCTGCCATTCGCAACAACG 1 191 702 Antisense
GGTGTTCAGACAGTTAGCATCGCGA 506 497 730 Antisense
TTAGCATCGCGAACAACACCAAGTC 25 683 743 Antisense
AAAACCTCTGACAATCTTCTGTACC 542 131 787 Antisense
TACCGTGTCAACCCAGTCTTTGGAT 29 647 808 Antisense
CGATGTGCTCCGTCACAATGGTGTT 155 249 861 Antisense
GTCTTAACATCCAATGCTTCATCAG 405 469 907 Antisense
CTTCGAGAGTGACCAACCACGCGAG 257 217 954 Antisense
Sequence Source Affymetrix Proprietary Database