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[182432_at]

Description H12D21.6 /REP_DB=WormBase Gene ID /WP=CE19919 /TR=Q9XTW8 /GB=CAB07430.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 21      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 55.7
(0.1)
A 		73.5
(1.4)
A 		16.1
(-3)
A 		93.6
(3)
A 		78
B 121.6
(3)
P 		10.5
(-3)
A 		28.2
(-2)
A 		92.5
(1.4)
A 		111
C 46.6
(-0.8)
A 		31.4
(-2.1)
A 		20.4
(-3)
A 		92
(3)
A 		72
avr 74.6
(1.5) 		38.5
(-1.6) 		21.6
(-3) 		92.7
(3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 131.6
(3)
A 		31
(-3)
A 		78.1
(-0.2)
P 		40.8
(-2.4)
A 		34.4
(-2.8)
A 		33.1
(-2.9)
A 		101
B 78.1
(1)
A 		64.2
(-0.3)
A 		100.5
(3)
P 		56.7
(-1)
A 		34.5
(-3)
A 		53
(-1.3)
A 		66
C 30.8
(-2.6)
A 		71.4
(2.7)
A 		54.9
(0.6)
A 		73.6
(3)
A 		27.5
(-3)
A 		50.3
(-0)
A 		46
avr 80.2
(3) 		55.5
(-0.1) 		77.8
(2.7) 		57
(0.1) 		32.1
(-3) 		45.5
(-1.3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 20.3
(3)
A 		16
(1.4)
A 		4.2
(-3)
A 		13.7
(0.5)
A 		16
B 38.8
(3)
P 		2.6
(-3)
A 		6.5
(-2.4)
A 		19.7
(-0.2)
A 		36
C 15.4
(2.9)
A 		9
(-1.6)
A 		7.1
(-3)
A 		15.5
(3)
A 		8
avr 24.8
(3) 		9.2
(-2) 		5.9
(-3) 		16.3
(0.3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 24.3
(3)
A 		11
(-1.9)
A 		17
(0.3)
P 		10.4
(-2.1)
A 		10.4
(-2.1)
A 		8.1
(-3)
A 		16
B 27.2
(3)
A 		21.4
(0.4)
A 		24.4
(1.7)
P 		15
(-2.5)
A 		14
(-3)
A 		19.8
(-0.4)
A 		13
C 11.4
(-1.8)
A 		21.8
(3)
A 		14
(-0.6)
A 		14.8
(-0.3)
A 		8.9
(-3)
A 		16.8
(0.7)
A 		13
avr 21
(3) 		18.1
(1.2) 		18.5
(1.5) 		13.4
(-1.6) 		11.1
(-3) 		14.9
(-0.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182432_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14347
Sequence Type Exemplar sequence
Representative Public ID H12D21.6 NCBI
Target Description H12D21.6 /REP_DB=WormBase Gene ID /WP=CE19919 /TR=Q9XTW8 /GB=CAB07430.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074272(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074272 NCBI Caenorhabditis elegans H12D21.6 (H12D21.6) mRNA, complete cds. 11/11 None
SNAP00000026965 ENSEMBL cdna:SNAP chromosome:CEL140:V:14899929:14900420:-1 11/11 None
GENEFINDER00000026972 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14899929:14900523:-1 10/11 None
H12D21.6 ENSEMBL cdna:known chromosome:CEL140:V:14899929:14900420:-1 gene:H12D21.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026963 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14899928-14900420(-) 93.5 100.0
Public Domain and Genome References
Gene Title
Ensembl H12D21.6
Entrez Gene 179988 Entrez gene
SwissProt Q9XTW8 EMBL-EBI
WormBase CE19919 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07430 Hypothetical protein H12D21.6 [Caenorhabditis elegans] ref|NP_506673.1| H12D21.6 [Caenorhabditis elegans] 1.0E-29
blast CAB07430 Hypothetical protein H12D21.6 [Caenorhabditis elegans] ref|NP_506673.1| H12D21.6 [Caenorhabditis elegans] 6.0E-26
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026972 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:182432_AT
gctctccactaaattagcaggtcttattttccttattgcactttcaacagctgctcccca
gaagaaaactaatgtgaccagacctcctccatctgagaaaactgtcgaaaaagaacagat
tgtcccacaaaactcagagaagttagaaaaaacggaaaaatcagaaaggacaagacagga
aagacaatctggatttggtggacaaagtcagcaatctagtggattcggaggaagcagctc
aaatggaatgggaagttctaatagtcagggaggatttgggaaccaagaaagtcagtcttc
cggcatgggatcaaataaccaaagacagtcaggattcggtggacagatgggatcttctgg
atcttctggatcttctggatcctctagtcaatcaggttttggcggacaaatgggttcatc
cggtcaatcagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTCCACTAAATTAGCAGGTCTT 369 297 15 Antisense
GGTCTTATTTTCCTTATTGCACTTT 488 503 34 Antisense
TATTGCACTTTCAACAGCTGCTCCC 626 665 48 Antisense
GCTGCTCCCCAGAAGAAAACTAATG 99 297 64 Antisense
AAAACTAATGTGACCAGACCTCCTC 83 131 79 Antisense
AGACCTCCTCCATCTGAGAAAACTG 7 71 94 Antisense
GAACAGATTGTCCCACAAAACTCAG 458 351 127 Antisense
GAAAGTCAGTCTTCCGGCATGGGAT 121 361 301 Antisense
GATTCGGTGGACAGATGGGATCTTC 173 433 347 Antisense
TCTTCTGGATCCTCTAGTCAATCAG 648 617 385 Antisense
AAATGGGTTCATCCGGTCAATCAGG 442 119 422 Antisense
Sequence Source Affymetrix Proprietary Database