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[182423_s_at]

Description W02A11.2 /REP_DB=WormBase Gene ID /WP=CE24025 /TR=Q9U354 /GB=CAB04889.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 20      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 42      6grp_total > 40      4grp_total > 08      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 426
1 2 3 4 range
A 707.5
(-3)
P
722.8
(-2.1)
P
780.6
(1.5)
P
805
(3)
P
98
B 638.9
(-2.3)
P
612
(-3)
P
649.8
(-2)
P
828.5
(3)
P
217
C 587.8
(-3)
P
646.3
(-2.2)
P
693.1
(-1.5)
P
1013.5
(3)
P
426
avr 644.7
(-3)
660.4
(-2.6)
707.8
(-1.4)
882.3
(3)
238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 645
1 2 3 4 5 6 range
A 913.1
(0.1)
P
688.9
(-2)
P
1229.1
(3)
P
890.5
(-0.2)
P
635
(-2.5)
P
584.4
(-3)
P
645
B 1095.5
(1.6)
P
1060.9
(0.9)
P
1167.7
(3)
P
864.1
(-2.9)
P
883.4
(-2.5)
P
856.7
(-3)
P
311
C 739.4
(-2.1)
P
1060.5
(3)
P
729.7
(-2.2)
P
1047.2
(2.8)
P
682.1
(-3)
P
874.2
(0)
P
378
avr 916
(0.5)
936.8
(1)
1042.2
(3)
933.9
(0.9)
733.5
(-3)
771.8
(-2.3)
309
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 257.6
(3)
P
156.9
(-1.3)
P
204.1
(0.7)
P
117.8
(-3)
P
140
B 203.7
(3)
P
151.1
(-2.9)
P
150.6
(-3)
P
176.6
(-0.1)
P
53
C 194.6
(-1)
P
185.8
(-1.7)
P
241.6
(3)
P
170.9
(-3)
P
71
avr 218.6
(3)
164.6
(-2.1)
198.8
(1.1)
155.1
(-3)
64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 168.3
(-1.8)
P
245
(1.9)
P
267.7
(3)
P
225.9
(1)
P
191.6
(-0.6)
P
142.3
(-3)
P
125
B 381.2
(3)
P
353.1
(1.9)
P
283.6
(-0.8)
P
228.9
(-3)
P
358.9
(2.1)
P
319.5
(0.6)
P
152
C 274.1
(0.8)
P
323.6
(3)
P
185.7
(-3)
P
210.6
(-1.9)
P
220.7
(-1.5)
P
292.6
(1.7)
P
138
avr 274.5
(0.7)
307.2
(3)
245.7
(-1.3)
221.8
(-3)
257.1
(-0.5)
251.5
(-0.9)
85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182423_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.732
Sequence Type Exemplar sequence
Representative Public ID W02A11.2 NCBI
Target Description W02A11.2 /REP_DB=WormBase Gene ID /WP=CE24025 /TR=Q9U354 /GB=CAB04889.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060829(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_060829 NCBI Caenorhabditis elegans W02A11.2 (W02A11.2) mRNA, complete cds. 11/11 None
SNAP00000035444 ENSEMBL cdna:SNAP chromosome:CEL140:I:12739252:12744708:-1 11/11 None
GENEFINDER00000035456 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12741540:12744708:-1 11/11 None
W02A11.2 ENSEMBL cdna:known chromosome:CEL140:I:12741422:12744731:-1 gene:W02A11.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12741470-12744639(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02A11.2
Entrez Gene 173143 Entrez gene
SwissProt Q9U354 EMBL-EBI
WormBase CE24025 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603003_AT similar to Serine/threonine-protein kinase WNK4 (Protein kinase with no lysine 4) (Protein kinase, lysine-deficient 4) (Liver regeneration-related protein LRRG120) cfa
CANINE_2:CFAAFFX.22786.1.S1_AT similar to Serine/threonine-protein kinase WNK4 (Protein kinase with no lysine 4) (Protein kinase, lysine-deficient 4) (Liver regeneration-related protein LRRG120) cfa
CANINE_2:CFAAFFX.22786.1.S1_S_AT similar to Serine/threonine-protein kinase WNK4 (Protein kinase with no lysine 4) (Protein kinase, lysine-deficient 4) (Liver regeneration-related protein LRRG120) cfa
DROSOPHILA_2:1639625_AT dm
DROSGENOME1:146824_AT dm
CHICKEN:GGA.8289.1.S1_A_AT vacuolar protein sorting 25 (yeast) gga
HU35KSUBA:RC_AA451672_S_AT vacuolar protein sorting 25 (yeast) hs
U133_X3P:HS.108824.0.A1_3P_S_AT vacuolar protein sorting 25 (yeast) hs
HG-U133_PLUS_2:224608_S_AT vacuolar protein sorting 25 (yeast) hs
HG-U133B:224608_S_AT vacuolar protein sorting 25 (yeast) hs
HG-U95B:56405_AT vacuolar protein sorting 25 (yeast) hs
MOUSE430A_2:1428621_A_AT vacuolar protein sorting 25 (yeast) mm
MOE430A:1428621_A_AT vacuolar protein sorting 25 (yeast) mm
MOUSE430_2:1428621_A_AT vacuolar protein sorting 25 (yeast) mm
MU11KSUBA:AA616327_F_AT vacuolar protein sorting 25 (yeast) mm
MU11KSUBA:AA110660_G_AT vacuolar protein sorting 25 (yeast) mm
MU11KSUBA:AA110660_AT vacuolar protein sorting 25 (yeast) mm
MU11KSUBA:AA529827_F_AT vacuolar protein sorting 25 (yeast) mm
MG-U74AV2:95480_AT vacuolar protein sorting 25 (yeast) mm
MOE430A:1421051_S_AT vacuolar protein sorting 25 (yeast) mm
MOUSE430A_2:1421051_S_AT vacuolar protein sorting 25 (yeast) mm
MOUSE430_2:1421051_S_AT vacuolar protein sorting 25 (yeast) mm
MOE430A:1421050_AT vacuolar protein sorting 25 (yeast) mm
MOUSE430A_2:1421050_AT vacuolar protein sorting 25 (yeast) mm
MOUSE430_2:1421050_AT vacuolar protein sorting 25 (yeast) mm
MU19KSUBB:TC33334_AT Vacuolar protein sorting 25 (yeast), mRNA (cDNA clone MGC:35627 IMAGE:4240500) mm
Protein Similarities
Method ID Description E-Value
blast CAB04894 Hypothetical protein W02A11.1 [Caenorhabditis elegans] emb|CAB04711.1| Hypothetical protein W02A11.1 [Caenorhabditis elegans] ref|NP_493229.1| W02A11.1 [Caenorhabditis elegans] 1.0E-108
blast CAB04889 Hypothetical protein W02A11.2 [Caenorhabditis elegans] ref|NP_493230.1| W02A11.2 [Caenorhabditis elegans] 1.0E-104
blast CAE67991 Hypothetical protein CBG13601 [Caenorhabditis briggsae] 1.0E-101
blast CAE67992 Hypothetical protein CBG13602 [Caenorhabditis briggsae] 1.0E-100
blast CAB04889 Hypothetical protein W02A11.2 [Caenorhabditis elegans] ref|NP_493230.1| W02A11.2 [Caenorhabditis elegans] 9.0E-99
blast XP_787383 PREDICTED: similar to DNA segment Chr 11 Wayne State University 68 expressed (21.5 kD) (1N326) [Strongylocentrotus purpuratus] 3.0E-50
blast CAB04889 Hypothetical protein W02A11.2 [Caenorhabditis elegans] ref|NP_493230.1| W02A11.2 [Caenorhabditis elegans] 1.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR008570 EMBL-EBI Eukaryotic protein of unknown function DUF852 2.3E-112
Pfam IPR008570 EMBL-EBI Eukaryotic protein of unknown function DUF852 5.1E-103
Pfam IPR008570 EMBL-EBI Eukaryotic protein of unknown function DUF852 3.3E-30
Pfam IPR008570 EMBL-EBI Eukaryotic protein of unknown function DUF852 1.3E-57
Sequence
Target Sequence
>C. ELEGANS:182423_S_AT
gcttcagcattcaaatggccttggcaatacgattttccgccattttttaccattcaaaag
tctttgaacacaaaagacaagcaacttgaagcctgggctcgtttggtaattgattatgct
caacacaataagatttattcgttggatattgctgaagcaactaccagtgaattattcaat
aatcaaaagcttaatcgccgcctctcaaccgacggtgtcaacactgttctacagtatttg
gagcaaaaaaaactaatcgaattcacggacaacggccgtacacgtttccacattttctgg
cgtcgacccgatgtttgggccaatatgatctaccaatgggcggttgagaatgcatttatc
aatacaccactcactttatacgagattacccacggagatgatactaca


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GCTTCAGCATTCAAATGGCCTTGGC 587 305 34 Antisense
GGCCTTGGCAATACGATTTTCCGCC 360 541 50 Antisense
GACAAGCAACTTGAAGCCTGGGCTC 488 363 109 Antisense
GAAGCCTGGGCTCGTTTGGTAATTG 281 345 121 Antisense
GCCTCTCAACCGACGGTGTCAACAC 693 279 233 Antisense
GACGGTGTCAACACTGTTCTACAGT 357 377 244 Antisense
TTCACGGACAACGGCCGTACACGTT 598 637 295 Antisense
CGTCGACCCGATGTTTGGGCCAATA 593 239 334 Antisense
GGGCCAATATGATCTACCAATGGGC 506 491 350 Antisense
TCAATACACCACTCACTTTATACGA 477 633 392 Antisense
GATTACCCACGGAGATGATACTACA 529 429 417 Antisense
Sequence Source Affymetrix Proprietary Database