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[182416_at]

Description F53F4.12 /REP_DB=WormBase Gene ID /WP=CE23766 /TR=Q20727 /GB=CAB01211.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 91      6grp_total > 78      4grp_total > 14      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 205.2
(-1)
P 		179.1
(-3)
P 		196.5
(-1.6)
P 		255.9
(3)
P 		77
B 172.8
(-1.2)
P 		207.2
(3)
P 		158.5
(-3)
P 		200.5
(2.2)
P 		49
C 276.7
(3)
P 		204.1
(-3)
P 		226.1
(-1.2)
P 		233.9
(-0.5)
P 		73
avr 218.2
(1) 		196.8
(-2.5) 		193.7
(-3) 		230.1
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 262.2
(0)
P 		235.1
(-1.8)
P 		281.1
(1.3)
P 		217.3
(-3)
P 		262.1
(0)
P 		305.9
(3)
P 		89
B 213.2
(-2.6)
P 		263.2
(0.4)
P 		252.6
(-0.2)
P 		207.1
(-3)
P 		253.7
(-0.2)
P 		306
(3)
P 		99
C 201.4
(-2.3)
P 		207.8
(-2)
P 		181.2
(-3)
P 		208.1
(-2)
P 		224.5
(-1.4)
P 		346.5
(3)
P 		165
avr 225.6
(-2.2) 		235.4
(-1.6) 		238.3
(-1.5) 		210.8
(-3) 		246.8
(-1) 		319.5
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 74.7
(3)
P 		38.9
(-2.8)
P 		51.4
(-0.8)
P 		37.5
(-3)
P 		37
B 55.1
(3)
P 		51.2
(1.7)
P 		36.7
(-3)
P 		42.7
(-1)
P 		18
C 91.6
(3)
P 		58.7
(-0.8)
P 		78.8
(1.5)
P 		39.4
(-3)
P 		52
avr 73.8
(3) 		49.6
(-1.3) 		55.6
(-0.2) 		39.9
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 48.3
(-3)
P 		83.6
(3)
P 		61.2
(-0.8)
P 		55.1
(-1.8)
P 		79.1
(2.2)
P 		74.5
(1.5)
P 		35
B 74.2
(-1)
P 		87.6
(0.3)
P 		61.3
(-2.3)
P 		54.8
(-3)
P 		103.1
(1.9)
P 		114.1
(3)
P 		59
C 74.7
(-0.3)
P 		63.4
(-1.3)
P 		46.1
(-2.7)
P 		41.8
(-3)
P 		72.6
(-0.5)
P 		116
(3)
P 		74
avr 65.7
(-1.2) 		78.2
(0.3) 		56.2
(-2.3) 		50.6
(-3) 		84.9
(1) 		101.5
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182416_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12593
Sequence Type Exemplar sequence
Representative Public ID F53F4.12 NCBI
Target Description F53F4.12 /REP_DB=WormBase Gene ID /WP=CE23766 /TR=Q20727 /GB=CAB01211.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073976(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073976 NCBI Caenorhabditis elegans F53F4.12 (F53F4.12) mRNA, complete cds. 10/11 None
SNAP00000012448 ENSEMBL cdna:SNAP chromosome:CEL140:V:13628639:13629806:1 10/11 None
F53F4.12 ENSEMBL cdna:known chromosome:CEL140:V:13628639:13630002:1 gene:F53F4.12 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13628638-13629806(+) 99.9 99.9
Public Domain and Genome References
Gene Title
Ensembl F53F4.12
Entrez Gene 179851 Entrez gene
SwissProt Q20727 EMBL-EBI
WormBase CE36385 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01211 Hypothetical protein F53F4.12 [Caenorhabditis elegans] ref|NP_506377.2| F53F4.12 [Caenorhabditis elegans] 1.0E-170
blast CAE66273 Hypothetical protein CBG11517 [Caenorhabditis briggsae] 9.0E-92
Sequence
Target Sequence 
>C. ELEGANS:182416_AT
aaagaagctgcatccacagcaccgatcccattgaacgggaaagaaaaacgagaggtacaa
cggacactcggtgcgaaatgtctaccgcttttacatggtgttaataagcgaagaatgtgt
aaaaagagaaaagaagctacgccagctcaatttcgagacaacgaagaaggaatattggaa
catggagaaggttcttcttcttcagttcaacatcagcaggtcaaagatgacaggtcatcg
aacaaaataaatgaaaaaactggggaagttcaggaagattttcaagaaacacccagaaaa
cgtcggaaagcagcgagcaagtttccaccattttcatctccaacgcaacagcctcttttg
gtacgaccattgccgcccatgtattacgtccgcacgggcatggcttcgcaagttcttcca
caccagcatcatcatcatgattctcatcatcattcaatagattattatgaggatgtagag
attgaagaagaagctagagaacacgtattcgacgatgaagagctcatttcgggtgaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGAAGCTGCATCCACAGCACCGA 662 123 418 Antisense
CACAGCACCGATCCCATTGAACGGG 710 195 432 Antisense
AGAGGTACAACGGACACTCGGTGCG 247 53 468 Antisense
GTGCGAAATGTCTACCGCTTTTACA 132 479 488 Antisense
AGCTACGCCAGCTCAATTTCGAGAC 588 79 552 Antisense
CTTCTTCTTCAGTTCAACATCAGCA 525 221 611 Antisense
GAAAGCAGCGAGCAAGTTTCCACCA 491 359 723 Antisense
CTCCAACGCAACAGCCTCTTTTGGT 557 233 755 Antisense
CCTCTTTTGGTACGACCATTGCCGC 425 267 769 Antisense
GCCCATGTATTACGTCCGCACGGGC 505 287 792 Antisense
TGAAGAGCTCATTTCGGGTGACCAG 81 575 933 Antisense
Sequence Source Affymetrix Proprietary Database