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[182398_s_at]

Description H12D21.8 /REP_DB=WormBase Gene ID /WP=CE19921 /TR=Q9XTX1 /GB=CAB07426.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      6grp_highest_nonorm > 90      6grp_total > 26      4grp_total > 22      6grp_highest_sub > 01_02      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 219.4
(2.4)
P 		114.1
(-1.1)
P 		57.8
(-3)
A 		238.1
(3)
A 		180
B 212.2
(3)
P 		120.6
(-2)
A 		101.7
(-3)
A 		151.9
(-0.3)
P 		111
C 177.7
(0.8)
P 		117
(-1.7)
P 		86.8
(-3)
M 		229.8
(3)
P 		143
avr 203.1
(2.8) 		117.2
(-1.3) 		82.1
(-3) 		206.6
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 267.5
(3)
P 		260.8
(2.8)
P 		73.7
(-3)
P 		118.5
(-1.6)
P 		99.3
(-2.2)
P 		134.1
(-1.1)
P 		194
B 231.6
(3)
P 		178.7
(0.7)
P 		146.7
(-0.8)
P 		96.2
(-3)
P 		104.8
(-2.6)
P 		141.7
(-1)
P 		135
C 244
(3)
P 		237.8
(2.8)
P 		138.1
(-0.7)
P 		122.2
(-1.3)
P 		73.8
(-3)
P 		110.6
(-1.7)
P 		170
avr 247.7
(3) 		225.8
(2.2) 		119.5
(-2) 		112.3
(-2.2) 		92.6
(-3) 		128.8
(-1.6) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 79.9
(3)
P 		24.8
(-2.1)
P 		15.1
(-3)
A 		34.8
(-1.2)
A 		65
B 67.7
(3)
P 		29.8
(-2.2)
A 		23.6
(-3)
A 		32.4
(-1.8)
P 		44
C 58.8
(3)
P 		33.6
(-2.3)
P 		30.2
(-3)
M 		38.8
(-1.2)
P 		29
avr 68.8
(3) 		29.4
(-2.2) 		23
(-3) 		35.3
(-1.4) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 49.3
(-0.4)
P 		92.8
(3)
P 		16.1
(-3)
P 		30
(-1.9)
P 		30
(-1.9)
P 		32.6
(-1.7)
P 		77
B 80.6
(3)
P 		59.5
(0.7)
P 		35.6
(-1.9)
P 		25.5
(-3)
P 		42.6
(-1.1)
P 		52.8
(-0)
P 		55
C 90.4
(3)
P 		72.6
(1.4)
P 		35.1
(-2)
P 		24.6
(-2.9)
P 		23.9
(-3)
P 		37
(-1.8)
P 		67
avr 73.4
(2.8) 		75
(3) 		28.9
(-2.7) 		26.7
(-3) 		32.2
(-2.3) 		40.8
(-1.2) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182398_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14565
Sequence Type Exemplar sequence
Representative Public ID H12D21.8 NCBI
Target Description H12D21.8 /REP_DB=WormBase Gene ID /WP=CE19921 /TR=Q9XTX1 /GB=CAB07426.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074274(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074274 NCBI Caenorhabditis elegans H12D21.8 (H12D21.8) mRNA, complete cds. 11/11 A 
SNAP00000026969 ENSEMBL cdna:SNAP chromosome:CEL140:V:14906498:14909089:1 11/11 None
GENEFINDER00000026980 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14905672:14909089:1 11/11 None
F25G6.1 ENSEMBL cdna:known chromosome:CEL140:V:8578730:8582528:1 gene:F25G6.1 10/11 None
H12D21.8 ENSEMBL cdna:known chromosome:CEL140:V:14905672:14909192:1 gene:H12D21.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001562 5/11 Cross Hyb Matching Probes None
GENEFINDER00000001566 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Public Domain and Genome References
Gene Title
Ensembl F25G6.1
H12D21.8
Entrez Gene 259665 Entrez gene
SwissProt Q9XTX1 EMBL-EBI
WormBase CE19921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07426 Hypothetical protein H12D21.8 [Caenorhabditis elegans] ref|NP_506675.1| H12D21.8 [Caenorhabditis elegans] 0.0
blast AAK84524 Hypothetical protein F25G6.1 [Caenorhabditis elegans] ref|NP_505211.3| F25G6.1 [Caenorhabditis elegans] 0.0
blast CAC14422 Hypothetical protein Y71A12B.7 [Caenorhabditis elegans] ref|NP_493432.1| Y71A12B.7 [Caenorhabditis elegans] 0.0
blast T33287 hypothetical protein H20H17.1 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:182398_S_AT
gattcagtgaaacagcctctcatcgatccgaaaagtggtcacaaattatctgatagaatc
ggatgtggccaatcatcaaataatcgtaaaaaaaaagttgctgaattatcaacccttacg
gtctacaagaacaataccaactccgatgacgacacaggaaccgcaaatagtagagaaaat
ctagatcaatcacttccggatgcatcgatgagtgacaatttttcgcctatcttctcgtct
acgatgatcaatggaagcagacgatcaagcagatcacgtaacagcaccaaagtcatggat
ccatcaccatctgatacaaacgagtctgacgaaaccagtggaaattgtgatgtgttcgtt
aatcgcacacgtcttctgtttgacgatgatggggacgattcaacggtcactctcttcaac
aacacagagaaaactcttcaattcaaatgtagaattgattctgatgagtactctgtgaag
ccgactcgcggaaatattaagccaaatatggataagacgatgagagttcgagtaggtgat
actgctcctggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCAGTGAAACAGCCTCTCATCG 314 431 1864 Antisense
ACAGCCTCTCATCGATCCGAAAAGT 396 109 1875 Antisense
GAATTATCAACCCTTACGGTCTACA 552 317 1966 Antisense
GATCAATCACTTCCGGATGCATCGA 646 421 2047 Antisense
TGAGTGACAATTTTTCGCCTATCTT 431 567 2072 Antisense
GCCTATCTTCTCGTCTACGATGATC 178 283 2088 Antisense
GCAGATCACGTAACAGCACCAAAGT 517 311 2132 Antisense
AGTCATGGATCCATCACCATCTGAT 115 61 2154 Antisense
TAATCGCACACGTCTTCTGTTTGAC 479 635 2223 Antisense
TGGGGACGATTCAACGGTCACTCTC 210 553 2253 Antisense
TCGAGTAGGTGATACTGCTCCTGGC 387 601 2391 Antisense
Sequence Source Affymetrix Proprietary Database