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[182384_at]

Description T10D4.4 /REP_DB=WormBase Gene ID /WP=CE20044 /TR=Q9TZF3 /GB=AAC68717.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 24      6grp_cgc > 55      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 3.8
(-3)
A 		13.8
(-1.6)
A 		25.5
(-0.1)
A 		48.1
(3)
A 		44
B 47.1
(3)
A 		14.6
(-3)
A 		41.8
(2)
A 		29.9
(-0.2)
A 		33
C 8.4
(-2.7)
A 		37.9
(3)
A 		11.2
(-2.2)
A 		6.8
(-3)
A 		31
avr 19.8
(-3) 		22.1
(-1.4) 		26.2
(1.5) 		28.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 20.6
(-1.6)
A 		27.5
(-0.8)
A 		60.9
(3)
A 		8.4
(-3)
A 		9.2
(-2.9)
A 		10.2
(-2.8)
A 		53
B 1.8
(-3)
A 		39.4
(3)
A 		37.3
(2.7)
A 		33.4
(2)
A 		4.1
(-2.6)
A 		29.7
(1.5)
A 		38
C 9.1
(-2.8)
A 		10.9
(-2.2)
A 		8.3
(-3)
A 		8.5
(-2.9)
A 		28.6
(3)
A 		24
(1.6)
A 		20
avr 10.5
(-3) 		25.9
(0.7) 		35.5
(3) 		16.8
(-1.5) 		14
(-2.2) 		21.3
(-0.4) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.4
(-3)
A 		3
(-1.3)
A 		6.7
(2.7)
A 		7
(3)
A 		6
B 15
(3)
A 		3.6
(-3)
A 		9.7
(0.2)
A 		6.4
(-1.5)
A 		11
C 2.8
(-2)
A 		10.9
(3)
A 		3.9
(-1.3)
A 		1.1
(-3)
A 		10
avr 6.4
(1.8) 		5.8
(0) 		6.8
(3) 		4.8
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 3.8
(-2.1)
A 		9.8
(1.1)
A 		13.3
(3)
A 		2.1
(-3)
A 		2.8
(-2.6)
A 		2.5
(-2.8)
A 		11
B 0.6
(-3)
A 		13.1
(3)
A 		9.1
(1.1)
A 		8.9
(1)
A 		1.6
(-2.5)
A 		11.1
(2)
A 		13
C 3.4
(-1.7)
A 		3.3
(-1.7)
A 		2.1
(-2.7)
A 		1.7
(-3)
A 		9.3
(3)
A 		8
(2)
A 		8
avr 2.6
(-3) 		8.7
(3) 		8.2
(2.5) 		4.2
(-1.4) 		4.6
(-1) 		7.2
(1.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182384_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5963
Sequence Type Exemplar sequence
Representative Public ID T10D4.4 NCBI
Target Description T10D4.4 /REP_DB=WormBase Gene ID /WP=CE20044 /TR=Q9TZF3 /GB=AAC68717.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062053(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062053 NCBI Caenorhabditis elegans INSulin related family member (ins-31) (ins-31) mRNA, complete cds. 11/11 None
SNAP00000032455 ENSEMBL cdna:SNAP chromosome:CEL140:II:3123501:3127785:1 11/11 None
GENEFINDER00000032473 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3126627:3127785:1 11/11 None
T10D4.4 ENSEMBL cdna:known chromosome:CEL140:II:3127036:3127785:1 gene:T10D4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3127034-3127784(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-31
Ensembl T10D4.4
Entrez Gene 173660 Entrez gene
SwissProt Q9TZF3 EMBL-EBI
WormBase CE20044 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68713 Serpentine receptor, class h protein 71 [Caenorhabditis elegans] ref|NP_494453.1| Serpentine Receptor, class H family member (srh-71) [Caenorhabditis elegans] 1.0E-145
blast AAC68717 Insulin related protein 31 [Caenorhabditis elegans] ref|NP_494454.1| INSulin related family member (ins-31) [Caenorhabditis elegans] 1.0E-113
blast AAC68717 Insulin related protein 31 [Caenorhabditis elegans] ref|NP_494454.1| INSulin related family member (ins-31) [Caenorhabditis elegans] 1.0E-112
blast CAD56599 Hypothetical protein T21B4.14 [Caenorhabditis elegans] ref|NP_496670.2| Serpentine Receptor, class H family member (srh-73) [Caenorhabditis elegans] 4.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.1E-39
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.2E-38
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.1E-39
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.2E-38
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.3E-121
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.1E-39
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 4.2E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000032455 6 32-54,66-88,110-132,153-172,220-242,296-313
Sequence
Target Sequence 
>C. ELEGANS:182384_AT
gtccaccactttgaccattcaatgtttgccagaccggagaaaacgtgtggaggactactc
attcgtcgtgtcgatagaatttgcccgaatctaaattatacatataaaattgagtgggaa
cttatggacaactgttgcgaagtggtttgcgaggaccagtggattaaggaaaccttttgc
agagcgcccaggttcaactttttcggaccttcattcaaagcccttgaaagatcgtgtgga
ccaaaactgttcacaagggttaaaactgtgtgcggtgaagacatcaatgttgataataaa
gtcaagatttcggatcactgctgcacaccagagggaggatgcacagacgactggatcaag
gagaacgtctgcaaacagaccagattcaactttttccgacaatttctcgattcccctcaa
agatcatgtggaccccagttgttcaaaagagtgaatactttgtgtaatgaaaatatcaat
gttgaaaataatgtaagcgtgtcgaaaagctgttgcgaatcagcggcaggatgcacggat
gattgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCACCACTTTGACCATTCAATGT 417 477 64 Antisense
GACCATTCAATGTTTGCCAGACCGG 283 383 76 Antisense
TGGAGGACTACTCATTCGTCGTGTC 390 547 111 Antisense
TCGTCGTGTCGATAGAATTTGCCCG 20 601 126 Antisense
TAAGGAAACCTTTTGCAGAGCGCCC 139 637 228 Antisense
TCAACTTTTTCGGACCTTCATTCAA 48 629 257 Antisense
TCACTGCTGCACACCAGAGGGAGGA 239 625 378 Antisense
GACCAGATTCAACTTTTTCCGACAA 35 383 441 Antisense
TCTCGATTCCCCTCAAAGATCATGT 508 611 468 Antisense
GATCATGTGGACCCCAGTTGTTCAA 458 419 485 Antisense
GCGGCAGGATGCACGGATGATTGGA 665 293 586 Antisense
Sequence Source Affymetrix Proprietary Database