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[182376_s_at]

Description F53F4.11 /REP_DB=WormBase Gene ID /WP=CE20849 /TR=Q20720 /GB=CAB01204.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 56      6grp_total > 28      4grp_total > 04      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5747
1 2 3 4 range
A 3438.2
(-1.9)
P 		2594
(-3)
P 		3218.6
(-2.2)
P 		7293.7
(3)
P 		4700
B 4103.3
(-1)
P 		2857.2
(-2.3)
P 		2222
(-3)
P 		7969
(3)
P 		5747
C 3495.2
(-2.1)
P 		2944
(-3)
P 		3149.5
(-2.7)
P 		6657
(3)
P 		3713
avr 3678.9
(-1.8) 		2798.4
(-3) 		2863.4
(-2.9) 		7306.6
(3) 		4508

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1434
1 2 3 4 5 6 range
A 3077.8
(3)
P 		2168
(-1)
P 		1755.2
(-2.8)
P 		1701.8
(-3)
P 		2064
(-1.4)
P 		2511.2
(0.5)
P 		1376
B 2820.5
(3)
P 		2006
(-1.2)
P 		1910.7
(-1.6)
P 		1644
(-3)
P 		1756
(-2.4)
P 		1991.6
(-1.2)
P 		1177
C 2288.1
(3)
P 		1807
(-3)
P 		1829.1
(-2.7)
P 		2217.8
(2.1)
P 		1879.3
(-2.1)
P 		2048.6
(0)
P 		481
avr 2728.8
(3) 		1993.7
(-1.9) 		1831.7
(-3) 		1854.5
(-2.8) 		1899.8
(-2.5) 		2183.8
(-0.6) 		897
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1183
1 2 3 4 range
A 1251.8
(3)
P 		563.1
(-3)
P 		841.4
(-0.6)
P 		1067.7
(1.4)
P 		689
B 1308.5
(1)
P 		705.7
(-2)
P 		514.9
(-3)
P 		1698.2
(3)
P 		1183
C 1157
(3)
P 		846.4
(-3)
P 		1097.7
(1.9)
P 		1122.5
(2.3)
P 		311
avr 1239.1
(2.4) 		705.1
(-3) 		818
(-1.9) 		1296.1
(3) 		591

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 599
1 2 3 4 5 6 range
A 567.4
(-0.1)
P 		771
(3)
P 		382.2
(-3)
P 		431.6
(-2.2)
P 		622.8
(0.7)
P 		611.4
(0.5)
P 		389
B 981.4
(3)
P 		667.7
(-0.4)
P 		464
(-2.7)
P 		435.4
(-3)
P 		713.4
(0.1)
P 		742.7
(0.4)
P 		546
C 848.1
(3)
P 		551.4
(-1.4)
P 		465.5
(-2.7)
P 		446
(-3)
P 		608.1
(-0.6)
P 		685.8
(0.6)
P 		402
avr 799
(3) 		663.4
(0.8) 		437.2
(-3) 		437.7
(-3) 		648.1
(0.5) 		680
(1) 		362
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182376_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14639
Sequence Type Exemplar sequence
Representative Public ID F53F4.11 NCBI
Target Description F53F4.11 /REP_DB=WormBase Gene ID /WP=CE20849 /TR=Q20720 /GB=CAB01204.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073974(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073974 NCBI Caenorhabditis elegans F53F4.11 (F53F4.11) mRNA, complete cds. 11/11 None
SNAP00000012446 ENSEMBL cdna:SNAP chromosome:CEL140:V:13624898:13627113:-1 11/11 None
GENEFINDER00000012452 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13624898:13627113:-1 11/11 None
F53F4.11.1 ENSEMBL cdna:known chromosome:CEL140:V:13624568:13627113:-1 gene:F53F4.11 11/11 A 
F53F4.11.2 ENSEMBL cdna:known chromosome:CEL140:V:13624568:13627117:-1 gene:F53F4.11 11/11 A 
F53F4.11.3 ENSEMBL cdna:known chromosome:CEL140:V:13624697:13627116:-1 gene:F53F4.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13624897-13627113(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F4.11
Entrez Gene 179849 Entrez gene
SwissProt Q20720 EMBL-EBI
WormBase CE20849 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631636_AT dm
DROSGENOME1:142984_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01204 Hypothetical protein F53F4.11 [Caenorhabditis elegans] ref|NP_506375.1| F53F4.11 [Caenorhabditis elegans] 0.0
blast CAE66275 Hypothetical protein CBG11519 [Caenorhabditis briggsae] 1.0E-170
Sequence
Target Sequence 
>C. ELEGANS:182376_S_AT
gtgtcaatgttggtcacttgggtcaaagttctgctgatcttaaagaaaatatcgatacag
ttctcgagaagattgcttctaaatgtccaggaggctttgccaatattcgcagcatttact
tgggtgcaagcgatggtaaaccatcacttccaatttatgttgacaatggttcggcaacgg
aaattactcttccaactggatctgtacgtgataacaatccactcaaggaaacttcagaca
agtgctccacactcccagatggactcaaaattgctgttcgtaagaacgctcgcatccgtg
ttctcaaggaagataccgatcagtctgtactgttcccaactgtgcatgatgagcattcgg
atcgtgatcgcctgaagccaaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTCAATGTTGGTCACTTGGGTCA 569 477 1103 Antisense
GATTGCTTCTAAATGTCCAGGAGGC 78 435 1173 Antisense
GGAGGCTTTGCCAATATTCGCAGCA 520 515 1192 Antisense
ATTCGCAGCATTTACTTGGGTGCAA 393 7 1207 Antisense
AACTTCAGACAAGTGCTCCACACTC 348 139 1332 Antisense
TCCACACTCCCAGATGGACTCAAAA 657 587 1348 Antisense
GCTGTTCGTAAGAACGCTCGCATCC 629 295 1375 Antisense
TACCGATCAGTCTGTACTGTTCCCA 606 647 1416 Antisense
ACTGTTCCCAACTGTGCATGATGAG 629 95 1431 Antisense
GATGAGCATTCGGATCGTGATCGCC 353 411 1450 Antisense
GATCGTGATCGCCTGAAGCCAAAGA 499 417 1462 Antisense
Sequence Source Affymetrix Proprietary Database