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[182353_at]

Description C54D1.3 /REP_DB=WormBase Gene ID /WP=CE06979 /TR=Q18820 /GB=AAC48153.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 58      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 91      6grp_total > 47      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 318
1 2 3 4 range
A 280
(1.1)
P 		189.9
(-1)
P 		108.3
(-3)
P 		357.6
(3)
P 		249
B 285.1
(0.3)
P 		192.7
(-1.6)
P 		126.4
(-3)
P 		412.2
(3)
P 		286
C 300.2
(1.6)
P 		93.8
(-3)
P 		130.5
(-2.2)
P 		364.7
(3)
P 		271
avr 288.4
(0.9) 		158.8
(-2.1) 		121.7
(-3) 		378.2
(3) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 247
1 2 3 4 5 6 range
A 263.4
(0.4)
P 		342.9
(2.4)
P 		122
(-3)
P 		178.2
(-1.6)
P 		178.2
(-1.6)
P 		368.9
(3)
P 		247
B 222.8
(-1.4)
P 		244.1
(-0.4)
P 		229.6
(-1.1)
P 		186.8
(-3)
P 		200.3
(-2.4)
P 		321.4
(3)
P 		135
C 267
(0.7)
P 		276.9
(1)
P 		185
(-2.4)
P 		167.4
(-3)
P 		195.3
(-2)
P 		330.4
(3)
P 		163
avr 251.1
(-0.3) 		288
(1.1) 		178.9
(-2.9) 		177.5
(-3) 		191.3
(-2.5) 		340.2
(3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 101.9
(3)
P 		41.2
(-1.9)
P 		28.3
(-3)
P 		52.3
(-1)
P 		74
B 90.9
(3)
P 		47.6
(-1.2)
P 		29.3
(-3)
P 		87.8
(2.7)
P 		62
C 99.4
(3)
P 		27
(-3)
P 		45.5
(-1.5)
P 		61.5
(-0.1)
P 		72
avr 97.4
(3) 		38.6
(-2.6) 		34.4
(-3) 		67.2
(0.1) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 48.5
(-1.6)
P 		122
(3)
P 		26.6
(-3)
P 		45.2
(-1.8)
P 		53.8
(-1.3)
P 		89.8
(1)
P 		95
B 77.5
(-0.6)
P 		81.3
(-0.3)
P 		55.8
(-2.5)
P 		49.5
(-3)
P 		81.4
(-0.3)
P 		119.9
(3)
P 		70
C 98.9
(2.1)
P 		84.5
(1)
P 		47.1
(-2)
P 		33.7
(-3)
P 		63.2
(-0.7)
P 		110.6
(3)
P 		77
avr 75
(0) 		95.9
(2) 		43.2
(-3) 		42.8
(-3) 		66.1
(-0.8) 		106.8
(3) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182353_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16953
Sequence Type Exemplar sequence
Representative Public ID C54D1.3 NCBI
Target Description C54D1.3 /REP_DB=WormBase Gene ID /WP=CE06979 /TR=Q18820 /GB=AAC48153.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076800(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076800 NCBI Caenorhabditis elegans Insulin receptor SubsTrate homolog family member (ist-1) (ist-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000023891 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7131052:7139218:1 11/11 None
SNAP00000023883 ENSEMBL cdna:SNAP chromosome:CEL140:X:7135300:7139218:1 11/11 None
C54D1.3 ENSEMBL cdna:known chromosome:CEL140:X:7132155:7139598:1 gene:C54D1.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7132153-7139217(+) 96.53 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ist-1
Ensembl C54D1.3
Entrez Gene 180978 Entrez gene
SwissProt Q18820 EMBL-EBI
WormBase CE37098 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48153 Insulin receptor substrate homolog protein 1 [Caenorhabditis elegans] ref|NP_509201.3| Insulin receptor SubsTrate homolog family member (ist-1) [Caenorhabditis elegans] 0.0
blast AAL40865 insulin receptor substrate-like protein [Caenorhabditis elegans] 0.0
blast CAE65846 Hypothetical protein CBG10979 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:182353_AT
gaattgctgattgtacgcccagttcaagtttttctgggaagaaagatcacaaaaatgatg
gttcttctgattctgcatacatggacaaagaagggttgaagtacttggcagatatcaaaa
aacggaaaaaagagcaaggtgccatagacacaacaaattcctcctcctcctcgttaagca
caatagtttcgattgaagataacaaagtgaccagaagaatcacggaaaccgttgaaacaa
aagttgtgactgcggatctagcgaaagcacttggtgcaattgacccgaatagaagatctt
cagcatgcattgagataaatcgaaaaccatcgatttgtacagcaataatggaagaaagag
aaggagaagcatctgagaccgattctaggggagaaccatccgacaggaagacgaccgcgg
cccctgtaactcggaaacctgcatcgactggcagcagcatgagcccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTGCTGATTGTACGCCCAGTTC 25 325 3002 Antisense
TGTACGCCCAGTTCAAGTTTTTCTG 671 559 3013 Antisense
GGTTCTTCTGATTCTGCATACATGG 328 507 3061 Antisense
CATAGACACAACAAATTCCTCCTCC 340 213 3144 Antisense
CCTCCTCGTTAAGCACAATAGTTTC 194 267 3167 Antisense
AAGTTGTGACTGCGGATCTAGCGAA 68 157 3242 Antisense
AGCACTTGGTGCAATTGACCCGAAT 279 77 3267 Antisense
GAAGCATCTGAGACCGATTCTAGGG 607 341 3367 Antisense
ATCCGACAGGAAGACGACCGCGGCC 599 37 3399 Antisense
GTAACTCGGAAACCTGCATCGACTG 601 461 3427 Antisense
TCGACTGGCAGCAGCATGAGCCCGT 383 597 3445 Antisense
Sequence Source Affymetrix Proprietary Database