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[182327_at]

Description W08F4.6 /REP_DB=WormBase Gene ID /WP=CE14722 /TR=O02106 /GB=AAC71171.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_high_8_8 > 54      6grp_highest_nonorm > 44      6grp_total > 71      4grp_total > 57      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5135
1 2 3 4 range
A 97
(-3)
A 		3457.9
(1.1)
P 		5074.4
(3)
P 		84.8
(-3)
A 		4990
B 99.8
(-3)
P 		4693.8
(2.4)
P 		5219.5
(3)
P 		120.3
(-3)
A 		5120
C 96.8
(-3)
A 		4316.9
(2.1)
P 		5038.9
(3)
P 		142.1
(-2.9)
P 		4942
avr 97.9
(-3) 		4156.2
(1.9) 		5110.9
(3) 		115.7
(-3) 		5013

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2069
1 2 3 4 5 6 range
A 1418.7
(-1)
P 		2256.8
(1.7)
P 		1819.9
(0.3)
P 		2683.4
(3)
P 		1769.5
(0.1)
P 		764.9
(-3)
P 		1919
B 1461.2
(-0.2)
P 		2010.3
(1.6)
P 		1590.7
(0.2)
P 		2431.4
(3)
P 		1912.1
(1.3)
P 		614.1
(-3)
P 		1817
C 1491.1
(-0.4)
P 		1885.2
(0.8)
P 		2178
(1.8)
P 		2560.3
(3)
P 		2105.1
(1.5)
P 		690.9
(-3)
P 		1869
avr 1457
(-0.5) 		2050.8
(1.4) 		1862.9
(0.8) 		2558.4
(3) 		1928.9
(1) 		690
(-3) 		1868
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1744
1 2 3 4 range
A 35.3
(-2.9)
A 		750.6
(0.4)
P 		1326.6
(3)
P 		12.4
(-3)
A 		1314
B 31.8
(-3)
P 		1159.3
(2.7)
P 		1209.5
(3)
P 		25.6
(-3)
A 		1184
C 32
(-3)
A 		1241.1
(1.2)
P 		1756.2
(3)
P 		24
(-3)
P 		1732
avr 33
(-2.9) 		1050.3
(1.4) 		1430.8
(3) 		20.7
(-3) 		1410

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 616
1 2 3 4 5 6 range
A 261.5
(-2.3)
P 		802.6
(3)
P 		396.3
(-1)
P 		680.6
(1.8)
P 		534
(0.4)
P 		186.2
(-3)
P 		616
B 508.4
(0.1)
P 		669.2
(1.8)
P 		386.3
(-1.3)
P 		644
(1.5)
P 		776.9
(3)
P 		229
(-3)
P 		548
C 552.7
(1.3)
P 		575.2
(1.6)
P 		554.3
(1.3)
P 		514.9
(0.8)
P 		681.2
(3)
P 		231.3
(-3)
P 		450
avr 440.9
(-0.1) 		682.3
(3) 		445.6
(-0) 		613.2
(2.1) 		664
(2.8) 		215.5
(-3) 		467
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182327_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5112
Sequence Type Exemplar sequence
Representative Public ID W08F4.6 NCBI
Target Description W08F4.6 /REP_DB=WormBase Gene ID /WP=CE14722 /TR=O02106 /GB=AAC71171.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061358(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061358 NCBI Caenorhabditis elegans W08F4.6 (W08F4.6) mRNA, complete cds. 10/11 None
SNAP00000029462 ENSEMBL cdna:SNAP chromosome:CEL140:II:569504:572105:-1 10/11 None
GENEFINDER00000029474 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:569504:572105:-1 10/11 None
W08F4.6.2 ENSEMBL cdna:known chromosome:CEL140:II:569306:572108:-1 gene:W08F4.6 10/11 None
W08F4.6.1 ENSEMBL cdna:known chromosome:CEL140:II:569306:572110:-1 gene:W08F4.6 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:569503-572105(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mlt-8
Ensembl W08F4.6
Entrez Gene 173448 Entrez gene
SwissProt O02106 EMBL-EBI
WormBase CE37162 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71171 Hypothetical protein W08F4.6 [Caenorhabditis elegans] ref|NP_493759.2| W08F4.6 [Caenorhabditis elegans] 1.0E-160
blast CAE62886 Hypothetical protein CBG07073 [Caenorhabditis briggsae] 1.0E-156
blast AAC71171 Hypothetical protein W08F4.6 [Caenorhabditis elegans] ref|NP_493759.2| W08F4.6 [Caenorhabditis elegans] 1.0E-127
blast CAE62886 Hypothetical protein CBG07073 [Caenorhabditis briggsae] 1.0E-123
Sequence
Target Sequence 
>C. ELEGANS:182327_AT
taagcgaagatctcccactggacaaagttgaaatggacctggaagtacgtcacgccgtga
ccaataagcaaggatccaagggatggcaggtgattccatgtcagggatggaatattattg
atggttgtgatggagtcggctcgtggaacgttgtttattggatcactgcacaaaatgtta
gtactaatttagtaaaaaatgctgaaatgctccaaatttacagccgctactgtgacatgc
tcgacaaatgtaatgaagccgtttcgggagcccacaaatacgtaaaagaccgtaaagcca
acgatttcctgaaacagaacaagttgtgcccaccaccaaaggggcactggactatgactt
tctctaaagttttcagctctgaagatcttccaaaatccttctttggaccacttcaatcga
atgaatactggctaacattctccttcactgacggaaaagacaagaagctgggatgtgccc
gtttgtgggtggatgtatgcaagtaccaccttcaggacaagtcccaaaagtgcctccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAGCGAAGATCTCCCACTGGACAA 532 635 281 Antisense
AATGGACCTGGAAGTACGTCACGCC 272 163 312 Antisense
GATGGTTGTGATGGAGTCGGCTCGT 533 407 400 Antisense
TACAGCCGCTACTGTGACATGCTCG 543 643 499 Antisense
GCCGTTTCGGGAGCCCACAAATACG 512 263 538 Antisense
GCACTGGACTATGACTTTCTCTAAA 599 315 624 Antisense
AATCCTTCTTTGGACCACTTCAATC 644 165 674 Antisense
TAACATTCTCCTTCACTGACGGAAA 562 639 713 Antisense
GAAGCTGGGATGTGCCCGTTTGTGG 387 343 744 Antisense
GTATGCAAGTACCACCTTCAGGACA 648 437 775 Antisense
AGGACAAGTCCCAAAAGTGCCTCCG 196 1 794 Antisense
Sequence Source Affymetrix Proprietary Database