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[182312_at]

Description C05D2.5 /REP_DB=WormBase Gene ID /WP=CE26863 /TR=O45134 /GB=AAC02722.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 40      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 56.4
(-2.3)
A 		103.5
(1.8)
A 		117.6
(3)
A 		47.9
(-3)
A 		70
B 37.6
(-3)
A 		40
(-2.8)
A 		99.8
(3)
A 		63.8
(-0.5)
A 		62
C 37
(-3)
A 		83.9
(-0.2)
A 		137.7
(3)
A 		120.4
(2)
A 		101
avr 43.7
(-3) 		75.8
(-0.4) 		118.4
(3) 		77.4
(-0.3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 432
1 2 3 4 5 6 range
A 24.4
(-3)
A 		25.6
(-3)
A 		24.1
(-3)
A 		131.8
(-1.4)
A 		296
(0.9)
A 		439.7
(3)
P 		416
B 65.2
(-2.1)
A 		8.2
(-3)
A 		28
(-2.7)
A 		143.1
(-0.9)
A 		272.3
(1.1)
P 		393.1
(3)
P 		385
C 87.6
(-1.7)
A 		24.2
(-3)
A 		124.6
(-1)
A 		250.8
(1.6)
A 		318.2
(3)
A 		241.9
(1.4)
P 		294
avr 59.1
(-2.3) 		19.3
(-3) 		58.9
(-2.3) 		175.2
(-0.2) 		295.5
(1.9) 		358.2
(3) 		339
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 20.5
(0.4)
A 		22.5
(0.9)
A 		30.7
(3)
A 		7
(-3)
A 		24
B 12
(-2)
A 		9.9
(-3)
A 		23.1
(3)
A 		13.6
(-1.3)
A 		13
C 12.2
(-3)
A 		24.1
(-1)
A 		48
(3)
A 		20.3
(-1.6)
A 		36
avr 14.9
(-2.6) 		18.8
(-1.5) 		33.9
(3) 		13.6
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 4.5
(-3)
A 		9.1
(-2.7)
A 		5.2
(-3)
A 		33.4
(-1.3)
A 		89.3
(2)
A 		107.1
(3)
P 		103
B 22.7
(-2.2)
A 		2.7
(-3)
A 		6.8
(-2.8)
A 		37.9
(-1.5)
A 		110.6
(1.5)
P 		146.6
(3)
P 		144
C 32.5
(-1.4)
A 		7.4
(-3)
A 		31.7
(-1.5)
A 		50.4
(-0.3)
A 		103
(3)
A 		81
(1.6)
P 		96
avr 19.9
(-2.2) 		6.4
(-3) 		14.6
(-2.5) 		40.6
(-1) 		101
(2.4) 		111.6
(3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5993
Sequence Type Exemplar sequence
Representative Public ID C05D2.5 NCBI
Target Description C05D2.5 /REP_DB=WormBase Gene ID /WP=CE26863 /TR=O45134 /GB=AAC02722.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065806(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065806 NCBI Caenorhabditis elegans Germline-enhanced AT-HooK protein family member (gak-1) (gak-1) mRNA, complete cds. 11/11 None
SNAP00000007734 ENSEMBL cdna:SNAP chromosome:CEL140:III:5604413:5607301:1 11/11 None
GENEFINDER00000007744 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5604413:5607301:1 11/11 None
C05D2.5 ENSEMBL cdna:known chromosome:CEL140:III:5604412:5607499:1 gene:C05D2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5603667-5607300(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gak-1
Ensembl C05D2.5
Entrez Gene 175776 Entrez gene
SwissProt O45134 EMBL-EBI
WormBase CE26863 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC02722 Germline-enhanced at-hook protein protein 1 [Caenorhabditis elegans] ref|NP_498207.1| Germline-enhanced AT-HooK protein family member (gak-1) [Caenorhabditis elegans] 0.0
blast CAE64296 Hypothetical protein CBG08971 [Caenorhabditis briggsae] 1.0E-144
Sequence
Target Sequence 
>C. ELEGANS:182312_AT
agaccatgcgagctccacatgtactcactcagttctactcagttaatcaggcatcgatgg
ctcgtaatagggaaggttctgatggtccttcttcctcagcagcaagaccggttggaagac
cacccactacgcaaccagtagaaactgcagtggagaaaaagaagaatgatgaagatgaga
aaagacatcatccactcacaaacttcacaacagcatccacgtcttcccaaaatctgcaag
atcaaccaccacccaagaataccgttctccgctggtcaaatatcacaactccgaggattc
ttcgtccaccaactctcaaaactccctactcgcttcccaggactgcagtgattccggtga
tgaagaagaagcagcccgaaatagagcaagttcagccgaagaaggacacgacggcaagcc
ttgttcgcaaaagaggccgcccgagaaaggagaagcctctggaggtgcagtctcctcgca
agggactatatcttcgcttcaaatcgtgcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACCATGCGAGCTCCACATGTACT 219 71 1727 Antisense
GTACTCACTCAGTTCTACTCAGTTA 88 459 1747 Antisense
GAAGGTTCTGATGGTCCTTCTTCCT 49 335 1798 Antisense
GAAAAGACATCATCCACTCACAAAC 209 355 1905 Antisense
ACAAACTTCACAACAGCATCCACGT 587 111 1924 Antisense
CGTTCTCCGCTGGTCAAATATCACA 27 249 1989 Antisense
TCACAACTCCGAGGATTCTTCGTCC 662 627 2009 Antisense
CTTCCCAGGACTGCAGTGATTCCGG 609 219 2059 Antisense
AGGACACGACGGCAAGCCTTGTTCG 226 55 2129 Antisense
GAAGCCTCTGGAGGTGCAGTCTCCT 308 345 2178 Antisense
ATATCTTCGCTTCAAATCGTGCACA 137 21 2214 Antisense
Sequence Source Affymetrix Proprietary Database